Amplite® Colorimetric Glucose-6-Phosphate Assay Kit

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells. G6P may also be converted to glycogen or starch for storage in the liver and muscles. G6P is utilized by glucose-6-phosphate dehydrogenase (G6PD) to generate the reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. AAT Bioquest's Amplite® Colorimetric Glucose-6-Phosphate Assay Kit provides a simple, sensitive and rapid method for detecting G6P in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the coupled enzyme assay, the G6P concentration is proportionally related to NADPH that is specifically monitored by a chromogenic NADPH sensor. The absorption signal can be read by an absorption microplate reader at an absorbance ratio of A575 nm to A605 nm. With the Amplite® G6P Assay Kit, we were able to detect as little as 1 µM G6P in a 100 µL reaction volume.

G6P dose response was measured with Amplite® Colorimetric G6P Assay Kit in a 96-well white clear bottom plate using a SpectraMax Plus (Molecular Devices) microplate reader.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13805	200 Tests	Price

Citations

View all 2 citations: Citation Explorer

Reprogramming of glycometabolism caused by low-level lead in vascular smooth muscle cells

Authors:

Xu, Li-Hui and Duan, Huan-ren and Zhang, Yu and Zhang, Tong-jia and Liu, Ya-shu and Zhou, Jin-sa and Hu, Wen-xiu and Zhang, Huan and Shi, Wan-ying and Sun, Su-ju

Enzyme Activities of Five White-Rot Fungi in the Presence of Nanocellulose

Authors:

Reyes, Carolina and Poulin, Alexandre and Nystr{\"o}m, Gustav and Schwarze, Francis WMR and Ribera, Javier

Journal:

Journal of Fungi (2021): 222

References

View all 84 references: Citation Explorer

The microsomal enzyme 17beta-hydroxysteroid dehydrogenase 3 faces the cytoplasm and uses NADPH generated by glucose-6-phosphate dehydrogenase

Authors:

Legeza B, Balazs Z, Nashev LG, Odermatt A.

Journal:

Endocrinology (2013): 205

Molecular characterization of glucose-6-phosphate dehydrogenase deficient variants in Baghdad city - Iraq

Authors:

Al-Musawi BM, Al-Allawi N, Abdul-Majeed BA, Eissa AA, Jubrael JM, Hamamy H.

Journal:

BMC Blood Disord (2012): 4

High prevalence of hemoglobin disorders and glucose-6-phosphate dehydrogenase (G6PD) deficiency in the Republic of Guinea (West Africa)

Authors:

Millimono TS, Loua KM, Rath SL, Relvas L, Bento C, Diakite M, Jarvis M, Daries N, Ribeiro LM, Manco L, Kaeda JS.

Journal:

Hemoglobin (2012): 25

Effects of laparoscopic Roux-en-Y gastric bypass on glucose-6 phosphate dehydrogenase activity in obese type 2 diabetics

Authors:

Schneider AM, Rawat D, Weinstein LS, Gupte SA, Richards WO.

Journal:

Surg Endosc (2012): 823

A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency

Authors:

Shah SS, Diakite SA, Traore K, Diakite M, Kwiatkowski DP, Rockett KA, Wellems TE, Fairhurst RM.

Journal:

Sci Rep (2012): 299

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

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Page updated on September 25, 2025

Component A: Enzyme Probe	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NADP	1 vial
Component D: Glucose-6-Phosphate Standard	1 vial (3.04 mg)