Amplite® Colorimetric Glucose-6-Phosphate Assay Kit

Amplite® Colorimetric Glucose-6-Phosphate Assay Kit is a colorimetric assay kit for quantifying glucose-6-phosphate (G6P) in biological samples through an enzyme-coupled NADPH readout.

Colorimetric detection: Uses a chromogenic NADPH sensor to generate a signal proportional to G6P concentration.
High sensitivity: Detects G6P concentrations as low as 1 µM in a 100 µL reaction volume.
Enzyme-coupled chemistry: Converts G6P into a proportional NADPH signal for quantitative measurement.
Ratiometric readout: Measure absorbance at 575/605 nm using a clear-bottom plate.
Applications: Suitable for G6P quantitation in serum, plasma, urine, and cell culture samples.

G6P dose response was measured with Amplite® Colorimetric G6P Assay Kit in a 96-well white clear bottom plate using a SpectraMax Plus (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13805	200 Tests	Price

Overview

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells. G6P may also be converted to glycogen or starch for storage in the liver and muscles. G6P is utilized by glucose-6-phosphate dehydrogenase (G6PD) to generate the reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. AAT Bioquest's Amplite® Colorimetric Glucose-6-Phosphate Assay Kit provides a simple, sensitive and rapid method for detecting G6P in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the coupled enzyme assay, the G6P concentration is proportionally related to NADPH that is specifically monitored by a chromogenic NADPH sensor. The absorption signal can be read by an absorption microplate reader at an absorbance ratio of A575 nm to A605 nm. With the Amplite® G6P Assay Kit, we were able to detect as little as 1 µM G6P in a 100 µL reaction volume.

Citations

View all 3 citations: Citation Explorer

The coordinated actions of metformin in the intestine and liver improves hyperglycemia

Authors:

He, Ling and An, Hongying and Ramirez, Karina and Radovick, Sally and Wondisford, Fredric E

Journal:

Cell Reports (2026)

Reprogramming of glycometabolism caused by low-level lead in vascular smooth muscle cells

Authors:

Xu, Li-Hui and Duan, Huan-ren and Zhang, Yu and Zhang, Tong-jia and Liu, Ya-shu and Zhou, Jin-sa and Hu, Wen-xiu and Zhang, Huan and Shi, Wan-ying and Sun, Su-ju

Enzyme Activities of Five White-Rot Fungi in the Presence of Nanocellulose

Authors:

Reyes, Carolina and Poulin, Alexandre and Nystr{\"o}m, Gustav and Schwarze, Francis WMR and Ribera, Javier

Journal:

Journal of Fungi (2021): 222

References

View all 84 references: Citation Explorer

The microsomal enzyme 17beta-hydroxysteroid dehydrogenase 3 faces the cytoplasm and uses NADPH generated by glucose-6-phosphate dehydrogenase

Authors:

Legeza B, Balazs Z, Nashev LG, Odermatt A.

Journal:

Endocrinology (2013): 205

Molecular characterization of glucose-6-phosphate dehydrogenase deficient variants in Baghdad city - Iraq

Authors:

Al-Musawi BM, Al-Allawi N, Abdul-Majeed BA, Eissa AA, Jubrael JM, Hamamy H.

Journal:

BMC Blood Disord (2012): 4

High prevalence of hemoglobin disorders and glucose-6-phosphate dehydrogenase (G6PD) deficiency in the Republic of Guinea (West Africa)

Authors:

Millimono TS, Loua KM, Rath SL, Relvas L, Bento C, Diakite M, Jarvis M, Daries N, Ribeiro LM, Manco L, Kaeda JS.

Journal:

Hemoglobin (2012): 25

Effects of laparoscopic Roux-en-Y gastric bypass on glucose-6 phosphate dehydrogenase activity in obese type 2 diabetics

Authors:

Schneider AM, Rawat D, Weinstein LS, Gupte SA, Richards WO.

Journal:

Surg Endosc (2012): 823

A novel cytofluorometric assay for the detection and quantification of glucose-6-phosphate dehydrogenase deficiency

Authors:

Shah SS, Diakite SA, Traore K, Diakite M, Kwiatkowski DP, Rockett KA, Wellems TE, Fairhurst RM.

Journal:

Sci Rep (2012): 299

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

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Page updated on October 6, 2026

Component A: Enzyme Probe	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NADP	1 vial
Component D: Glucose-6-Phosphate Standard	1 vial (3.04 mg)