Name: Amplite® Colorimetric Glucose and Sucrose Assay Kit
Brand: AAT Bioquest
SKU: 40009
Price: 516 USD
Availability: InStock

Amplite® Colorimetric Glucose and Sucrose Assay Kit

Amplite® Colorimetric Glucose and Sucrose Assay Kit is a colorimetric assay kit for quantifying glucose and sucrose in biological, food, and cell culture samples using enzyme-coupled absorbance detection.

Colorimetric detection: Measures glucose through a glucose oxidase-based colorimetric product.
Dual-analyte quantitation: Measures free glucose directly and determines sucrose after invertase conversion.
Broad sample compatibility: Suitable for serum, plasma, body fluids, food, tissue extracts, and growth medium.
Microplate compatible: Read absorbance at 575 nm using a clear-bottom plate.
Applications: Suitable for metabolic profiling, glycolysis studies, and glucose/sucrose measurements in food samples.

Sucrose dose response was measured with Amplite® Colorimetric Glucose and Sucrose Assay Kit on a 96-well clear bottom microplate after 30 minutes of incubation using a ClarioStar microplate reader (BMG) at Abs=575 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
40009	100 Tests	Price

Overview

Glucose, a key molecule in cellular energy metabolism, primarily functions during cellular respiration. It undergoes glycolytic breakdown for immediate ATP production or is converted into glycogen for energy storage. Sucrose, a disaccharide, is broken down into glucose and fructose by the enzyme invertase. The Amplite® Colorimetric Glucose and Sucrose Assay Kit offers a convenient method for measuring glucose and sucrose levels in various biological samples, such as serum, plasma, body fluids, food, tissue extracts, and growth medium. This kit is useful for assessing aerobic glycolysis, pyruvate kinase activity, and metabolic profiles in different biological matrices. It is also employed for determining protein and sugar concentrations in food samples, as well as monitoring plasma insulin and glucose levels in murine models. To measure glucose levels, glucose oxidase specifically oxidizes free glucose, producing a colorimetric product (OD570nm) that is proportional to the glucose present. To measure sucrose levels, invertase is added to the reaction to convert sucrose into free glucose and fructose, allowing for the measurement of the total glucose level. The sucrose level is then calculated by subtracting the free glucose from the total glucose.

References

View all 50 references: Citation Explorer

Knockout of the sugar transporter OsSTP15 enhances grain yield by improving tiller number due to increased sugar content in the shoot base of rice (Oryza sativa L.).

Authors:

Li, Mingjuan and Li, Hongye and Zhu, Qidong and Liu, Dong and Li, Zhen and Chen, Haifei and Luo, Jinsong and Gong, Pan and Ismail, Abdelbagi M and Zhang, Zhenhua

Journal:

The New phytologist (2024): 1250-1265

Exploratory study on the metabolic similarity of denitrifying carbon sources.

Authors:

Zhang, Jinsen and Xia, Zhiheng and Wei, Qi and Luo, Fangzhou and Jiang, Zhao and Ao, Ziding and Chen, Huiling and Niu, Xiaoxu and Liu, Guo-Hua and Qi, Lu and Wang, Hongchen

Journal:

Environmental science and pollution research international (2024): 19961-19973

Adjusting function of camphor on primary metabolism in Cinnamomum camphora stressed by high temperature.

Authors:

Cai, Yuyan and Xu, Haozhe and Xu, Chenyi and Zuo, Zhaojiang

Journal:

Plant science : an international journal of experimental plant biology (2024): 111956

Response surface methodology use in construction of polianiline-coated carbon paste electrode-based biosensor: Modification and characterization.

Authors:

Yağız, Esra and Ozyilmaz, Gul and Ozyilmaz, Ali Tuncay

Journal:

Biotechnology and applied biochemistry (2024): 147-161

The Identification of a Strain for the Biological Purification of Soy Molasses to Produce Functional Soy Oligosaccharides and Optimize Purification Conditions.

Authors:

Yang, Ran and Ma, Jinghao and Wang, Zechen and Du, Yihua and Tian, Shubin and Fan, Guangsen and Liu, Xiaoyan and Teng, Chao

Journal:

Foods (Basel, Switzerland) (2024)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 nm
Recommended plate	Clear bottom

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Page updated on October 26, 2026

Component A: Amplite® Red	1 Vial
Component B: Sucrose Assay Buffer	25 mL
Component C: Invertase	1 Vial
Component D: Glucose Enzyme Mix	1 Bottle
Component E: Sucrose Standard	1 Vial
Component F: DMSO	1 Vial