Name: Amplite® Colorimetric Glucose Oxidase Assay Kit
Brand: AAT Bioquest
SKU: 11299
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Glucose Oxidase Assay Kit

Amplite® Colorimetric Glucose Oxidase Assay Kit is a colorimetric assay kit for measuring glucose oxidase activity in solution through a hydrogen peroxide-coupled absorbance readout.

Colorimetric detection: Uses Amplite® Red to detect hydrogen peroxide generated by glucose oxidase activity.
High sensitivity: Detects glucose oxidase down to 12.5 mU/mL.
Enzyme-coupled chemistry: Links beta-D-glucose oxidation to an HRP-dependent colorimetric signal.
Microplate compatible: Read absorbance at 570 nm in 96- or 384-well clear-bottom plates.
Applications: Suitable for glucose oxidase activity measurements and related biosensor or food research.

Glucose oxidase dose response was measured with Amplite® Colorimetric Glucose Oxidase Assay Kit (Cat#11299) on a 96-well clear bottom plate using a SpectraMax reader (Molecular Devices) with path check on.

Protocol

SDS

Catalog	Size	Price	Quantity
11299	500 Tests	Price

Overview

The glucose oxidase is a dimeric protein that catalyzes the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. It is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. Furthermore, Glucose oxidase is commonly used in biosensors to detect glucose. The Amplite® Glucose Oxidase Assay Kit provides a quick and sensitive method for the measurement of glucose oxidase in solution. It can be performed in a convenient 96-well or 384-well microtiter plate format and readily adapted to automation without a separation step. The kit uses our Amplite® Red substrate which can be monitored using an absorbance microplate reader at 570 nm.

Citations

View all 2 citations: Citation Explorer

A reassessment of the carnivorous status of salmonids: Hepatic glucokinase is expressed in wild fish in Kerguelen Islands

Authors:

Mar, undefined and el, Lucie and Gaudin, Philippe and Guéraud, Fran&ccaron;ois and Glise, Stéphane and Herman, Alex and re , undefined and Plagnes-Juan, Elisabeth and Véron, Vincent and Panserat, Stéphane and Labonne, Jacques

Journal:

Science of The Total Environment (2018): 276--285

Overcoming 5-Fu resistance in human non-small cell lung cancer cells by the combination of 5-Fu and cisplatin through the inhibition of glucose metabolism

Authors:

Zhao, Jun-gang and Ren, Kai-ming and Tang, Jun

Journal:

Tumor Biology (2014): 12305--12315

References

View all 29 references: Citation Explorer

Factors affecting accuracy and time requirements of a glucose oxidase-peroxidase assay for determination of glucose

Authors:

Hall MB, Keuler NS.

Journal:

J AOAC Int (2009): 50

Conformation and activity dependent interaction of glucose oxidase with CdTe quantum dots: towards developing a nanoparticle based enzymatic assay

Authors:

Priyam A, Chatterjee A, Bhattacharya SC, Saha A.

Journal:

Photochem Photobiol Sci (2009): 362

Using an indicator displacement assay to monitor glucose oxidase activity in blood serum

Authors:

Zhang T, Anslyn EV.

Journal:

Org Lett (2007): 1627

Glucose oxidase assisted homogeneous electrochemical receptor binding assay for drug screening

Authors:

Funabashi H, Tanaka Y, Imamura Y, Mie M, Manabe T, Tanaka H, Takahashi T, H and a H, Aizawa M, Kobatake E.

Journal:

Biosens Bioelectron (2006): 1675

1,4-Benzoquinone-based electrophoretic assay for glucose oxidase

Authors:

Urban PL, Goodall DM, Bergstrom ET, Bruce NC.

Journal:

Anal Biochem (2006): 35

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570 nm
Recommended plate	Clear bottom

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Page updated on October 4, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Horseradish Peroxidase (HRP)	1 vial
Component D: Glucose Oxidase	1 vial (100 units)
Component E: DMSO	1 vial (200 µL)
Component F: Glucose	1 vial