Amplite® Colorimetric Glucose Quantitation Kit

Amplite® Colorimetric Glucose Quantitation Kit is a colorimetric assay kit for quantifying glucose through a glucose oxidase-coupled hydrogen peroxide readout in a microplate format.

Colorimetric detection: Uses Amplite® Red to measure hydrogen peroxide generated by glucose oxidase-mediated reactions.
High sensitivity: Detects D-glucose concentrations as low as 3 µM after 30 minutes.
Enzyme-coupled chemistry: Converts glucose into a proportional hydrogen peroxide signal for quantitative measurement.
Low-background readout: Measure absorbance at approximately 570 nm in a clear-bottom plate.
Applications: Suitable for glucose quantitation across metabolic and other biological research workflows.

Glucose dose response was measured with Amplite® Colorimetric Glucose Quantitation Kit (Cat #40004) on a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices) with path check on. As low as 3 uM glucose was detected with 30 minutes incubation (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
40004	500 Tests	Price

Overview

Glucose, a monosaccharide, is the most important carbohydrate in biology. It is a source of energy and metabolic intermediate for cell growth. As one of the main products of photosynthesis, glucose starts cellular respiration in both prokaryotes and eukaryotes. Glucose level is a key diagnostic parameter for many metabolic disorders, e.g., diabetes. This Amplite® Colorimetric Glucose Quantitation Kit provides a quick and sensitive method for the measurement of glucose. It uses glucose oxidase-based enzyme coupled reactions to detect glucose through the production of hydrogen peroxide, which is monitored by our Amplite® Red peroxidase substrate. Amplite® Red peroxidase substrate can be read by an absorbance microplate reader at ~570 nm. The assay is robust, and can be readily adapted for a wide variety of applications that require the measurement of glucose. The assay has very low background since it is run in the red visible range that significantly reduces the interference from biological samples. With the Amplite® Colorimetric Glucose Quantitation Kit, we can detect as little as 3 µM D-glucose.

Citations

View all 11 citations: Citation Explorer

Female Wistar Kyoto More Immobile rats with genetic stress hyper-reactivity show enhanced contextual fear memory without deficit in extinction of fear

Authors:

Harter, Aspen M and Nemesh, Mariya and Ji, Michelle T and Lee, Luca and Yamazaki, Anna and Kim, Chris and Redei, Eva E

Journal:

European Journal of Neuroscience (2024)

RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53

Authors:

Jacquier, Valentin and Gitenay, Delphine and Fritsch, Samuel and Bonnet, Sandrine and Gy{\H{o}}rffy, Bal{\'a}zs and Jalaguier, St{\'e}phan and Linares, Laetitia K and Cavaill{\`e}s, Vincent and Teyssier, Catherine

Journal:

Cellular and Molecular Life Sciences (2022): 1--17

Aspects {\'e}volutifs et environnementaux de la plasticit{\'e} ph{\'e}notypique chez deux Moronid{\'e}s, le bar Europ{\'e}en (Dicentrarchus labrax) et le bar ray{\'e} (Morone saxatilis)

Authors:

Gourtay, Clémence

MicroRNAs regulate gene plasticity during cold shock in zebrafish larvae

Authors:

Hung, I-Chen and Hsiao, Yu-Chuan and Sun, H Sunny and Chen, Tsung-Ming and Lee, Shyh-Jye

Journal:

BMC genomics (2016): 922

Early nutritional programming in fish: tailoring the metabolic use of dietary carbohydrates

Authors:

Rocha, Filipa Soares

References

View all 21 references: Citation Explorer

Insulin and glucose mediate opposite intracellular ionized magnesium variations in human lymphocytes

Authors:

Delva P, Degan M, Trettene M, Lechi A.

Journal:

J Endocrinol (2006): 711

Recombinant human glucose-6-phosphate dehydrogenase. Evidence for a rapid-equilibrium random-order mechanism

Authors:

Wang XT, Au SW, Lam VM, Engel PC.

Journal:

Eur J Biochem (2002): 3417

Fluorescent microplate cell assay to measure uptake and metabolism of glucose in normal human lung fibroblasts

Authors:

Leira F, Louzao MC, Vieites JM, Botana LM, Vieytes MR.

Journal:

Toxicol In Vitro (2002): 267

Glucose-induced alterations of intracellular ionized magnesium in human lymphocytes

Authors:

Delva P, Degan M, Pastori C, Faccini G, Lechi A.

Journal:

Life Sci (2002): 2119

Plasma glycohydrolase levels in patients with type 1 diabetes at onset and in subjects undergoing an intravenous glucose tolerance test

Authors:

Goi G, Bairati C, Burlina A, Massaccesi L, Monciotti C, Segalini G, Testa R, Lombardo A.

Journal:

Metabolism (2000): 1352

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570 nm
Recommended plate	White or Black wall/Clear bottom

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Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Horseradish Peroxidase (HRP)	1 vial (10 units)
Component D: Glucose Oxidase	1 vial (100 units)
Component E: DMSO	1 vial (200 µL)
Component F: Glucose	1 vial (144 mg)