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Amplite® Colorimetric Glucose Quantitation Kit
Glucose, a monosaccharide, is the most important carbohydrate in biology. It is a source of energy and metabolic intermediate for cell growth. As one of the main products of photosynthesis, glucose starts cellular respiration in both prokaryotes and eukaryotes. Glucose level is a key diagnostic parameter for many metabolic disorders, e.g., diabetes. This Amplite® Colorimetric Glucose Quantitation Kit provides a quick and sensitive method for the measurement of glucose. It uses glucose oxidase-based enzyme coupled reactions to detect glucose through the production of hydrogen peroxide, which is monitored by our Amplite® Red peroxidase substrate. Amplite® Red peroxidase substrate can be read by an absorbance microplate reader at ~570 nm. The assay is robust, and can be readily adapted for a wide variety of applications that require the measurement of glucose. The assay has very low background since it is run in the red visible range that significantly reduces the interference from biological samples. With the Amplite® Colorimetric Glucose Quantitation Kit, we can detect as little as 3 µM D-glucose.
Glucose dose response was measured with Amplite® Colorimetric Glucose Quantitation Kit (Cat #40004) on a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices) with path check on. As low as 3 uM glucose was detected with 30 minutes incubation (n=3).
Glucose dose response was measured with Amplite® Colorimetric Glucose Quantitation Kit (Cat #40004) on a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices) with path check on. As low as 3 uM glucose was detected with 30 minutes incubation (n=3).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
40004500 Tests
Price
 
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance570 nm
Recommended plateWhite or Black wall/Clear bottom
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Page updated on September 25, 2025