Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit

Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit is a colorimetric assay kit for measuring GAPDH activity in cell culture or tissue samples using a coupled enzymatic absorbance readout.

Colorimetric detection: Generates a 450 nm signal directly proportional to GAPDH activity.
Coupled enzyme chemistry: Measures GAPDH-catalyzed activity through formation of a colorimetric product.
Broad sample compatibility: Suitable for cell culture and tissue samples.
Microplate compatible: Read absorbance at 450 nm using 96- or 384-well clear-bottom plates.
Applications: Suitable for GAPDH activity measurements in glycolysis and cellular metabolism research.

The NADH dose response was measured using the Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit on a 96-well clear bottom microplate. The assay was incubated for 10 minutes and measured at 450 nm using a ClarioStar microplate reader (BMG).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11321	100 Tests	Price

Overview

The Amplite® Colorimetric GAPDH Activity Assay Kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalyzed by GAPDH. This reaction results in the formation of a colorimetric product with an absorbance at 450nm, which is directly proportional to the enzymatic activity of GAPDH present in cell culture or tissue samples. One unit (U) is the amount of enzyme that catalyzes the reaction of 1 µmol of substrate per minute. GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is a multifunctional protein that serves both as a glycolytic enzyme and as a uracil DNA glycosylase. In glycolysis, it catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphate glycerate. GAPDH is also involved in many cellular processes such as apoptosis, membrane trafficking, glucose and iron metabolism, and nuclear translocation. The expression of GAPDH in cells is constitutive, making it a housekeeping gene. Dysregulation of GAPDH activity has been associated with carcinogenesis, abnormal cell growth, and late-onset Alzheimer's disease. Quantification of GAPDH expression levels in different experimental conditions or disease states can provide insights into metabolic changes associated with cellular processes such as proliferation, differentiation, and response to stress.

References

View all 50 references: Citation Explorer

Triplet state spectroscopy reveals involvement of the buried tryptophan residue 310 in Glyceraldehyde-3-phosphate dehydrogenase (GAPD) in the interaction with acrylamide.

Authors:

Mukherjee, Priyanka and Mukhopadhyay, Titas Kumar and Mukherjee, Manini and Roy, Pritam and Ghosh, Rina and Sardar, Pinki Saha and Ghosh, Sanjib

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2024): 123622

Structure-based functional analysis of a novel NADPH-producing glyceraldehyde-3-phosphate dehydrogenase from Corynebacterium glutamicum.

Authors:

Son, Hyeoncheol Francis and Park, Woojin and Kim, Sangwoo and Kim, Il-Kwon and Kim, Kyung-Jin

Journal:

International journal of biological macromolecules (2024): 128103

Structure of glyceraldehyde-3-phosphate dehydrogenase from Paracoccidioides lutzii in complex with an aldonic sugar acid.

Authors:

Hernández-Prieto, Jonathan Heiler and Martini, Viviane Paula and Iulek, Jorge

Journal:

Biochimie (2024): 20-33

Glyceraldehyde 3-Phosphate Dehydrogenase on the Surface of Candida albicans and Nakaseomyces glabratus Cells-A Moonlighting Protein That Binds Human Vitronectin and Plasminogen and Can Adsorb to Pathogenic Fungal Cells via Major Adhesins Als3 and Epa6.

Authors:

Bednarek, Aneta and Satala, Dorota and Zawrotniak, Marcin and Nobbs, Angela H and Rapala-Kozik, Maria and Kozik, Andrzej

Journal:

International journal of molecular sciences (2024)

Role of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in autophagy activation following subarachnoid hemorrhage.

Authors:

Huo, Junfeng and Dong, Wei and Xu, Jiake and Ma, Lu and You, Chao

Journal:

Experimental neurology (2024): 114577

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	450 nm
Recommended plate	Clear bottom

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Page updated on September 28, 2026

Component A: GAPDH Probe	1 mL
Component B: GAPDH Assay Buffer	4 mL
Component C: GAPDH Substrate	1 Bottle
Component D: NADH Standard	1 Vial
Component E: GAPDH Positive Control	1 Vial