Name: Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
Brand: AAT Bioquest
SKU: 13838
Price: 415 USD
Availability: InStock

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit is a colorimetric assay kit for quantifying G3P through an enzyme-coupled hydrogen peroxide readout in a microplate format.

Colorimetric detection: Uses Amplite® Red to measure hydrogen peroxide generated by G3P enzyme-coupling reactions.
High sensitivity: Detects G3P concentrations as low as 12.5 µM in a 100 µL assay volume.
Mix-and-read workflow: Optimized for streamlined measurement without a separation step.
HTS compatible: Read absorbance at approximately 575 nm in 96- or 384-well clear-bottom plates.
Applications: Suitable for G3P quantitation in glycolysis, energy metabolism, and lipid metabolism research.

Glycerol 3-phosphate dose response was obtained with Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit in a 96-well clear bottom /black wall plate using a SpectraMax absorbance microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13838	200 Tests	Price

Overview

Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway. Animals, fungi, and plants use G3P to produce ATP. It is used to regenerate NAD+ in brain and skeletal muscle cells. G3P has been linked to lipid imbalance diseases such as obesity. Amplite® G3P Assay Kit provides one of the most sensitive methods for quantifying G3P. The kit uses Amplite® Red substrate to quantify the concentration of G3P, which is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. The kit is an optimized "mix and read" format that is compatible with HTS applications. It detects as little as 12.5 µM G3P in 100 µL assay volume as shown in Figure 1. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read at ~576 +/- 5 nm with an absorbance microplate reader.

Citations

View all 4 citations: Citation Explorer

A redox-sensitive phosphatase regulates glycolysis as a metabolic switch in the bacterial inner membrane

Authors:

Zheng, Lei and Niu, Wei and Xie, Xianfa and Vu, Trung and Du, Guangwei

Journal:

Science Advances (2026): eaea8724

The regulatory mechanism of the yeast osmoresponse under different glucose concentrations

Authors:

Shen, Wenting and Gao, Ziqing and Chen, Kaiyue and Zhao, Alusi and Ouyang, Qi and Luo, Chunxiong

Journal:

iScience (2022): 105809

EOS789, pan-phosphate transporter inhibitor, ameliorates the progression of kidney injury in anti-GBM-induced glomerulonephritis rats

Authors:

Tsuboi, Yoshinori and Ichida, Yasuhiro and Murai, Atsuko and Maeda, Akira and Iida, Manami and Kato, Atsuhiko and Ohtomo, Shuichi and Horiba, Naoshi

Journal:

Pharmacology Research \& Perspectives (2022): e00973

Glucose enhances catecholamine-stimulated lipolysis via increased glycerol-3-phosphate synthesis in 3T3-L1 adipocytes and rat adipose tissue

Authors:

Takeuchi, Nodoka and Higashida, Kazuhiko and Li, Xi and Nakai, Naoya

Journal:

Molecular Biology Reports (2021): 6269--6276

References

View all 24 references: Citation Explorer

Interaction of fosfomycin with the glycerol 3-phosphate transporter of Escherichia coli

Authors:

Santoro A, Cappello AR, Madeo M, Martello E, Iacopetta D, Dolce V.

Journal:

Biochim Biophys Acta (2011): 1323

CCAAT/enhancer binding protein-beta negatively regulates the expression of glycerol-3-phosphate dehydrogenase 1 in pig PK-15 cells

Authors:

Gao Y, Pan Y.

Journal:

J Appl Genet (2011): 451

Rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis

Authors:

Frohlich KM, Roberts RA, Housley NA, Audia JP.

Journal:

J Bacteriol (2010): 4281

Effects of salinity changes on the growth of Dunaliella salina and its isozyme activities of glycerol-3-phosphate dehydrogenase

Authors:

Chen H, Jiang JG, Wu GH.

Journal:

J Agric Food Chem (2009): 6178

Design and synthesis of small molecule glycerol 3-phosphate acyltransferase inhibitors

Authors:

Wydysh EA, Medghalchi SM, Vadlamudi A, Townsend CA.

Journal:

J Med Chem (2009): 3317

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B: Enzyme Mix	2 bottles (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Glycerol 3-Phosphate (G3P) Standard	1 vial (lyophilized powder)
Component E: DMSO	1 vial (100 µL)