Amplite® Colorimetric Glycerol Assay Kit

Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production. Amplite® Colorimetric Glycerol Assay Kit offers a sensitive assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using our Amplite® Red HRP substrate in the HRP-coupled reactions. The signal can be measured with an absorbance microplate reader using OD 575 nm. With this Colorimetric Glycerol Assay Kit, we were able to detect as low as 0.15 µg/mL (~1.6 µM) glycerol in a 100 µL reaction volume.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13832	200 Tests	Price

References

View all 47 references: Citation Explorer

Rapid monitoring of glycerol in fermentation growth media: Facilitating crude glycerol bioprocess development

Authors:

Abad S, Perez X, Planas A, Turon X.

Journal:

Talanta (2014): 210

Synthesis of substituted 1,3-diesters of glycerol using wittig chemistry

Authors:

Lowe HI, Toyang NJ, Watson CT, Bryant J.

Journal:

Nat Prod Commun (2014): 687

The glycoglycerolipid 1,2-dipalmitoyl-3-(N-palmitoyl-6'-amino-6'-deoxy-alpha-d-glucosyl)-sn-glycerol is no inhibitor of the human Myt1 kinase

Authors:

Rohe A, Gollner C, Erdmann F, Sippl W, Schmidt M.

Journal:

J Enzyme Inhib Med Chem (2014): 514

Functional characterization of Yersinia pestis aerobic glycerol metabolism

Authors:

Willias SP, Chauhan S, Motin VL.

Journal:

Microb Pathog (2014): 33

Quantitative analysis of glycerol levels in human urine by liquid chromatography-tandem mass spectrometry

Authors:

Dong Y, Ma Y, Yan K, Shen L, Wang X, Xu Y, He G, Wu Y, Lu J, Yang Z, Feng F.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2014): 30

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	575 nm
Recommended plate	Clear bottom
Instrument specification(s)	Path check

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 26, 2025

Component A: Amplite® Red HRP substrate (light sensitive)	1 vial
Component B: Enzyme Mix	2 bottles (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Glycerol Standard	80 µL/vial
Component E: DMSO	1 vial (100 µL)