Amplite® Colorimetric Glycerol Assay Kit

Amplite® Colorimetric Glycerol Assay Kit is a colorimetric assay kit for quantifying glycerol in biological samples through an enzyme-coupled hydrogen peroxide readout.

Colorimetric detection: Uses Amplite® Red to detect hydrogen peroxide generated by glycerol enzyme-coupling reactions.
High sensitivity: Detects glycerol as low as 0.15 µg/mL (~1.6 µM) in a 100 µL reaction.
Enzyme-coupled chemistry: Converts glycerol into a proportional HRP-dependent colorimetric signal.
Microplate compatible: Read absorbance at 575 nm using a clear-bottom microplate.
Applications: Suitable for glycerol measurements in metabolic and lipid metabolism research.

Glycerol dose response was measured with Amplite® Colorimetric Glycerol Assay Kit on a black wall/clear bottom 96-well plate using a SpectraMax reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13832	200 Tests	Price

Overview

Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production. Amplite® Colorimetric Glycerol Assay Kit offers a sensitive assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using our Amplite® Red HRP substrate in the HRP-coupled reactions. The signal can be measured with an absorbance microplate reader using OD 575 nm. With this Colorimetric Glycerol Assay Kit, we were able to detect as low as 0.15 µg/mL (~1.6 µM) glycerol in a 100 µL reaction volume.

References

View all 47 references: Citation Explorer

Rapid monitoring of glycerol in fermentation growth media: Facilitating crude glycerol bioprocess development

Authors:

Abad S, Perez X, Planas A, Turon X.

Journal:

Talanta (2014): 210

Synthesis of substituted 1,3-diesters of glycerol using wittig chemistry

Authors:

Lowe HI, Toyang NJ, Watson CT, Bryant J.

Journal:

Nat Prod Commun (2014): 687

The glycoglycerolipid 1,2-dipalmitoyl-3-(N-palmitoyl-6'-amino-6'-deoxy-alpha-d-glucosyl)-sn-glycerol is no inhibitor of the human Myt1 kinase

Authors:

Rohe A, Gollner C, Erdmann F, Sippl W, Schmidt M.

Journal:

J Enzyme Inhib Med Chem (2014): 514

Functional characterization of Yersinia pestis aerobic glycerol metabolism

Authors:

Willias SP, Chauhan S, Motin VL.

Journal:

Microb Pathog (2014): 33

Quantitative analysis of glycerol levels in human urine by liquid chromatography-tandem mass spectrometry

Authors:

Dong Y, Ma Y, Yan K, Shen L, Wang X, Xu Y, He G, Wu Y, Lu J, Yang Z, Feng F.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2014): 30

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 nm
Recommended plate	Clear bottom
Instrument specification(s)	Path check

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Page updated on October 5, 2026

Component A: Amplite® Red HRP substrate (light sensitive)	1 vial
Component B: Enzyme Mix	2 bottles (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Glycerol Standard	80 µL/vial
Component E: DMSO	1 vial (100 µL)