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Amplite® Colorimetric Glycerol Assay Kit
Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue. When fasting, triglycerides stored in these lipid droplets can be hydrolyzed to generate free glycerol and fatty acids. The amount of free glycerol released to the bloodstream is proportional to the triglyceride/fatty acid cycling rate, which is important in the metabolic regulation and heat production. Amplite® Colorimetric Glycerol Assay Kit offers a sensitive assay for measuring glycerol levels in biological samples. This assay is based on an enzyme coupled reaction of glycerol, in which the product hydrogen peroxide can be detected using our Amplite® Red HRP substrate in the HRP-coupled reactions. The signal can be measured with an absorbance microplate reader using OD 575 nm. With this Colorimetric Glycerol Assay Kit, we were able to detect as low as 0.15 µg/mL (~1.6 µM) glycerol in a 100 µL reaction volume.
Glycerol dose response was measured with Amplite® Colorimetric Glycerol Assay Kit on a black wall/clear bottom 96-well plate using a SpectraMax reader.
Glycerol dose response was measured with Amplite® Colorimetric Glycerol Assay Kit on a black wall/clear bottom 96-well plate using a SpectraMax reader.
CatalogSize
Price
Quantity
13832200 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Absorbance microplate reader
Absorbance575 nm
Recommended plateClear bottom
Instrument specification(s)Path check
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Page updated on October 13, 2025