The Amplite® Colorimetric Glycogen Assay Kit provides a streamlined assay for quantifying glycogen using a sensitive and reliable two-step enzymatic process. In the first step, glycogen is enzymatically hydrolyzed into glucose. In the second step, the released glucose undergoes an oxidation reaction, producing hydrogen peroxide, which then reacts with the proprietary Amplite® Red dye to generate a red-colored product. The intensity of this color is directly proportional to the glycogen concentration and can be easily measured at 575 nm using a standard absorbance microplate reader. This kit is compatible with a wide range of biological matrices, including cell lysates, tissue extracts, and serum samples. The protocol is easy to follow, and it produces a stable colorimetric signal, enabling flexible endpoint detection for high-throughput screening or routine glycogen analysis in metabolic research, diabetes studies, and other related applications.