Name: Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Brand: AAT Bioquest
SKU: 13845
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Colorimetric detection: Uses Oxirite™ Hypochlorite Sensor to generate a strong color signal upon reaction with hypochlorite.
High sensitivity: Detects sodium hypochlorite concentrations as low as 0.5 µM after 3-5 minutes.
High specificity: Selectively detects hypochlorite and hypochlorous acid.
Microplate compatible: Read absorbance at 555 ± 5 nm using a clear-bottom microplate.
Applications: Suitable for hypochlorite measurements in biological, toxicological, and environmental research.

Protocol

SDS

Catalog	Size	Price	Quantity
13845	200 Tests	Price

Overview

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance. Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit offers a sensitive absorption-based assay for measuring hypochlorite (hypochlorous acid) with high specificity. Upon selective reaction with hypochlorite (hypochlorous) the colorless Oxirite™ Hypochlorite Sensor generates a strong color product. The color signal can be measured by a absorption microplate reader at ~555 nm. With this Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit, trace amount of hypochlorite can be detected.

Citations

View all 4 citations: Citation Explorer

Two-component system HprS/HprR enhances kidney colonization of uropathogenic Escherichia coli CFT073 by increasing flagellar expression

Authors:

Li, Xueping and Pang, Yu and Liu, Zixiang and Mu, Jianwei and Wang, Sheng and Chen, Ziwei and Wang, Zhiyun and Wang, Tao

Journal:

Cell Reports (2026)

Myeloperoxidase and its derivative hypochlorous acid combined clinical indicators predict new-onset atrial fibrillation in sepsis: a case-control study

Authors:

Dai, Hui and Ye, Jiawei and Wang, Shangyuan and Li, Xingyao and Li, Wenjie

Journal:

BMC Cardiovascular Disorders (2024): 1--12

A MXene-Based Bionic Cascaded-Enzyme Nanoreactor for Tumor Phototherapy/Enzyme Dynamic Therapy and Hypoxia-Activated Chemotherapy

Authors:

Zhang, Xiaoge and Cheng, Lili and Lu, Yao and Tang, Junjie and Lv, Qijun and Chen, Xiaomei and Chen, You and Liu, Jie

Journal:

Nano-Micro Letters (2022): 1--21

Non-invasive Control of Bacterial Biofilms by Wireless Electrostimulation

Authors:

Wang, Hao and Tampio, Alex JF and Xu, Yikang and Nicholas, Brian D and Ren, Dacheng

Journal:

ACS Biomaterials Science & Engineering (2019)

References

View all 60 references: Citation Explorer

Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation

Authors:

Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.

Journal:

J Endod (2014): 51

Effect of hypochlorite oxidation on cholinesterase-inhibition assay of acetonitrile extracts from fruits and vegetables for monitoring traces of organophosphate pesticides

Authors:

Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.

Journal:

J Toxicol Sci (2014): 71

Green synthesis of carbon dots with down- and up-conversion fluorescent properties for sensitive detection of hypochlorite with a dual-readout assay

Authors:

Yin B, Deng J, Peng X, Long Q, Zhao J, Lu Q, Chen Q, Li H, Tang H, Zhang Y, Yao S.

Journal:

Analyst (2013): 6551

Comparative antimicrobial activities of aerosolized sodium hypochlorite, chlorine dioxide, and electrochemically activated solutions evaluated using a novel standardized assay

Authors:

Thorn RM, Robinson GM, Reynolds DM.

Journal:

Antimicrob Agents Chemother (2013): 2216

Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite

Authors:

Setlow B, Yu J, Li YQ, Setlow P.

Journal:

Lett Appl Microbiol (2013): 259

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	555 ± 5 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: Oxirite™ Hypochlorite Sensor (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Hypochlorite Standard	1 vial (300 µL)
Component D: DMSO	1 vial (600 µL)