Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
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Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
UNSPSC | 12352200 |
Overview | SDSProtocol |
Platform
Absorbance microplate reader
Absorbance | 555 ± 5 nm |
Recommended plate | Clear bottom |
Components
Example protocol
AT A GLANCE
Protocol summary
- Prepare Hypochlorite working solution (50 µL)
- Add Hypochlorite standards or test samples (50 µL)
- Incubate at room temperature for 3 - 5 min
- Monitor absorbance increase at OD of 555 ± 5 nm
Important notes
Thaw one vial of each kit component at room temperature before starting the experiment.
PREPARATION OF STOCK SOLUTION
1. Oxirite™ Hypochlorite Sensor stock solution (20X):
Add 500 µL of DMSO (Component D) into the vial of Oxirite™ Hypochlorite Sensor (Component A) to make 20X Oxirite™ Hypochlorite Sensor stock solution.
PREPARATION OF STANDARD SOLUTION
For convenience, use the Serial Dilution Planner: https://www.aatbio.com/tools/serial-dilution/13845
Add 100 μL of Hypochlorite Standard (Component C) into 400 μL of Assay Buffer (Component B) to get 1% Hypochlorite standard solution (H7). Take 1% Hypochlorite standard solution (H7) and perform 1:3 serial dilutions in Assay Buffer (Component B) get serially diluted Hypochlorite standards (H6 - H1).
PREPARATION OF WORKING SOLUTION
Add 250 µL of 20X Oxirite™ Hypochlorite Sensor stock solution into 5 mL of Assay Buffer (Component B), and mix well to make Hypochlorite working solution. Note: This Hypochlorite working solution is enough for one 96-well plate. It is not stable, use it promptly. Protect from light.
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. Layout of Hypochlorite standards and test samples in a 96-well clear bottom microplate. H = hypochlorite standard (H1 - H7, 0.001% to 1%), BL = blank control, TS = test sample.
BL | BL | TS | TS |
H1 | H1 | ... | ... |
H2 | H2 | ... | ... |
H3 | H3 | ||
H4 | H4 | ||
H5 | H5 | ||
H6 | H6 | ||
H7 | H7 |
Table 2. Reagent composition for each well.
Well | Volume | Reagent |
H1-H7 | 50 µL | Serial Dilution (0.001% to 1%) |
BL | 50 µL | Assay Buffer (Component B) |
TS | 50 µL | Test Sample |
- Prepare Hypochlorite standards (H), blank controls (BL), and test samples (TS) in a 96-well clear bottom microplate as provided in Table 1 and Table 2. For a 384-well plate, use 25 µL of reagent per well instead of 50 µL.
- Add 50 µL of Hypochlorite working solution to each well of Hypochlorite standards, blank control, and test samples to make the total Hypochlorite assay volume of 100 µL/well. For a 384-well plate, add 25 µL of working solution into each well instead, for a total volume of 50 µL/well.
- Incubate the reaction at room temperature for 3 - 5 minutes, protected from light.
- Monitor the absorbance increase with an absorbance plate reader at OD = 555 ± 5 nm.
Product Family
Name | Excitation (nm) | Emission (nm) |
Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit | 555 | 576 |
Images
Citations
Authors: Zhang, Xiaoge and Cheng, Lili and Lu, Yao and Tang, Junjie and Lv, Qijun and Chen, Xiaomei and Chen, You and Liu, Jie
Journal: Nano-Micro Letters (2022): 1--21
Authors: Wang, Hao and Tampio, Alex JF and Xu, Yikang and Nicholas, Brian D and Ren, Dacheng
Journal: ACS Biomaterials Science & Engineering (2019)
References
Authors: Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.
Journal: J Endod (2014): 51
Authors: Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.
Journal: J Toxicol Sci (2014): 71
Authors: Yin B, Deng J, Peng X, Long Q, Zhao J, Lu Q, Chen Q, Li H, Tang H, Zhang Y, Yao S.
Journal: Analyst (2013): 6551
Authors: Thorn RM, Robinson GM, Reynolds DM.
Journal: Antimicrob Agents Chemother (2013): 2216
Authors: Setlow B, Yu J, Li YQ, Setlow P.
Journal: Lett Appl Microbiol (2013): 259
Authors: Zhang J, Yang X.
Journal: Analyst (2013): 434
Authors: Takimoto K, Taharaguchi M, Sakai K, Takagi H, Tohya Y, Yamada YK.
Journal: Exp Anim (2013): 237
Authors: Perez-Cruz F, Cortes C, Atala E, Bohle P, Valenzuela F, Olea-Azar C, Speisky H, Aspee A, Lissi E, Lopez-Alarcon C, Bridi R.
Journal: Molecules (2013): 1638
Authors: Kanno A, Kawakami T, Takahashi Y, Onodera S.
Journal: J Toxicol Sci (2012): 389
Authors: Zhang J, Wang X, Yang X.
Journal: Analyst (2012): 2806
Application notes
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