Name: Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Brand: AAT Bioquest
SKU: 13845
Price: 348 USD
Availability: InStock

Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance. Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit offers a sensitive absorption-based assay for measuring hypochlorite (hypochlorous acid) with high specificity. Upon selective reaction with hypochlorite (hypochlorous) the colorless Oxirite™ Hypochlorite Sensor generates a strong color product. The color signal can be measured by a absorption microplate reader at ~580 nm. With this Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit, trace amount of hypochlorite can be detected.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13845	200 Tests	Price

Citations

View all 3 citations: Citation Explorer

Myeloperoxidase and its derivative hypochlorous acid combined clinical indicators predict new-onset atrial fibrillation in sepsis: a case-control study

Authors:

Dai, Hui and Ye, Jiawei and Wang, Shangyuan and Li, Xingyao and Li, Wenjie

Journal:

BMC Cardiovascular Disorders (2024): 1--12

A MXene-Based Bionic Cascaded-Enzyme Nanoreactor for Tumor Phototherapy/Enzyme Dynamic Therapy and Hypoxia-Activated Chemotherapy

Authors:

Zhang, Xiaoge and Cheng, Lili and Lu, Yao and Tang, Junjie and Lv, Qijun and Chen, Xiaomei and Chen, You and Liu, Jie

Journal:

Nano-Micro Letters (2022): 1--21

Non-invasive Control of Bacterial Biofilms by Wireless Electrostimulation

Authors:

Wang, Hao and Tampio, Alex JF and Xu, Yikang and Nicholas, Brian D and Ren, Dacheng

Journal:

ACS Biomaterials Science & Engineering (2019)

References

View all 60 references: Citation Explorer

Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation

Authors:

Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.

Journal:

J Endod (2014): 51

Effect of hypochlorite oxidation on cholinesterase-inhibition assay of acetonitrile extracts from fruits and vegetables for monitoring traces of organophosphate pesticides

Authors:

Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.

Journal:

J Toxicol Sci (2014): 71

A simple yet effective chromogenic reagent for the rapid estimation of bromate and hypochlorite in drinking water

Authors:

Zhang J, Yang X.

Journal:

Analyst (2013): 434

Use of pyrogallol red and pyranine as probes to evaluate antioxidant capacities towards hypochlorite

Authors:

Perez-Cruz F, Cortes C, Atala E, Bohle P, Valenzuela F, Olea-Azar C, Speisky H, Aspee A, Lissi E, Lopez-Alarcon C, Bridi R.

Journal:

Molecules (2013): 1638

Effect of hypochlorite-based disinfectants on inactivation of murine norovirus and attempt to eliminate or prevent infection in mice by addition to drinking water

Authors:

Takimoto K, Taharaguchi M, Sakai K, Takagi H, Tohya Y, Yamada YK.

Journal:

Exp Anim (2013): 237

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	555 ± 5 nm
Recommended plate	Clear bottom

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Fax
Email	sales@aatbio.com
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Custom size	Inquire
Technical Support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025

Component A: Oxirite™ Hypochlorite Sensor (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Hypochlorite Standard	1 vial (300 µL)
Component D: DMSO	1 vial (600 µL)