Name: Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit
Brand: AAT Bioquest
SKU: 13828
Price: 415 USD
Availability: InStock

Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit is a colorimetric assay kit for quantifying aspartate in biological samples through an enzyme-coupled hydrogen peroxide readout.

Colorimetric detection: Uses Amplite® Red to measure hydrogen peroxide generated from aspartate through an enzyme-coupled reaction.
Standard range: Supports aspartate standards from 12.5 to 100 µM.
Enzyme-coupled chemistry: Converts aspartate to pyruvate and generates a proportional hydrogen peroxide signal.
Microplate compatible: Read absorbance at 575 ± 5 nm using a clear-bottom microplate with path check.
Applications: Suitable for aspartate quantitation in metabolic and biochemical research samples.

Aspartate dose response was measured with Amplite® Colorimetric Aspartate Assay Kit on a white clear bottom 96-well plate using a SpectraMax microplate reader with Path check ON (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13828	200 Tests	Price

Overview

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid. Aspartate is involved in the control point of pyrimidine biosynthesis, in transamination reactions, interconversions with asparagine, in the metabolic pathway leading to AMP, in the urea cycle, and is a precursor to homoserine, threonine, isoleucine, and methionine. It is also involved in the malate aspartate shuttle. Amplite® Colorimetric Aspartate Assay Kit offers a sensitive colorimetric assay for quantifying aspartate in biological samples. Aspartate is converted to pyruvate that generates hydrogen peroxide through an enzyme coupled reaction. The amount of hydrogen peroxide generated by aspartate is monitored with Amplite® Red substrate for quantifying aspartate by in an absorbance microplate reader at 575 nm.

References

View all 20 references: Citation Explorer

A systematic and mechanistic evaluation of aspartic acid as filler for directly compressed tablets containing trimethoprim and trimethoprim aspartate

Authors:

ElShaer A, Hanson P, Mohammed AR.

Journal:

Eur J Pharm Biopharm (2013): 468

Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase

Authors:

Kato S, Ikuta T, Hemmi H, Takahashi S, Kera Y, Yoshimura T.

Journal:

Biosci Biotechnol Biochem (2012): 2150

Distribution of free D-aspartic acid and D-aspartate oxidase in frog Rana esculenta tissues

Authors:

Di Giovanni M, Burrone L, Chieffi Baccari G, Topo E, Santillo A.

Journal:

J Exp Zool A Ecol Genet Physiol (2010): 137

Thyroid hormones and D-aspartic acid, D-aspartate oxidase, D-aspartate racemase, H2O2, and ROS in rats and mice

Authors:

Topo E, Fisher G, Sorricelli A, Errico F, Usiello A, D'Aniello A.

Journal:

Chem Biodivers (2010): 1467

Aslfm, the D-aspartate ligase responsible for the addition of D-aspartic acid onto the peptidoglycan precursor of Enterococcus faecium

Authors:

Bellais S, Arthur M, Dubost L, Hugonnet JE, Gutmann L, van Heijenoort J, Legr and R, Brouard JP, Rice L, Mainardi JL.

Journal:

J Biol Chem (2006): 11586

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 ± 5 nm
Recommended plate	Clear bottom
Instrument specification(s)	Path check

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Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: Conversion Mix	2 vials (lyophilized powder)
Component E: Aspartate Standard	1 vial (lyophilized powder)
Component F: DMSO	1 vial (100 μL)