Amplite® Colorimetric L-Lactate Assay Kit

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid. Lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in the process of metabolism and exercise. Monitoring lactate levels is a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is not metabolized by mammals and its elimination from the body depends mainly on renal excretion. D- and L-lactic acid are found in many fermented milk products such as yoghurt and cheese, and also in pickled vegetables, and cured meats and fish. The D- and L-lactic acid (generated by bacteria) is a quality indicator of foods, such as egg, milk, fruit juice and wine. Abnormal high concentration of D-lactate in the blood is usually a reflection of bacterial overgrowth in the gastrointestinal tract. AAT Bioquest's Amplite® Lactate Assay Kits (Cat# 13814 and 13815 for L-lactate assay, and Cat# 13810 and 13811 for D-lactate assay) provide both fluorescence and absorbance-based method for detecting either L-lactate or D-lactate in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, lactate is proportionally related to NADH, which is specifically monitored by a chromogenic NADH sensor. The signal can easily read by an absorbance microplate reader at the absorbance ratio of ~A575nm/A605nm to increase assay sensitivity. With this Colorimetric Amplite® L-Lactate Assay Kit, we were able to detect as little as 4 µM L-lactate in a 100 µL reaction volume.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13815	200 Tests	Price

Citations

View all 38 citations: Citation Explorer

Effects of esketamine on electrophysiology and metabolic reprogramming in brain organoids: insights into antidepressant mechanisms

Authors:

Li, Mengjie and Duan, Wen and Hao, Xiaoyan and Li, Shuangjie and Wang, Chen and Liang, Yuanyuan and Hu, Zhengwei and Ma, Dongrui and Guo, Mengnan and Zuo, Chunyan and others,

Journal:

Molecular Psychiatry (2025): 1--12

Platelet-derived exosomal LINC00183 facilitate colorectal cancer malignant progression driven by histone lactylation through stabilizing ENO1

Authors:

Su, Guoqing and Qian, Jinghang and Wang, Yi and Zhu, Yu and Chen, Yanyan and Shen, Jingru and Jiang, Jiayuan and Cao, Yuepeng and Wang, Nannan and Huang, Xing and others,

Journal:

Cell Death \& Disease (2025): 593

TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth

Authors:

Xia, Tian and Meng, Lian and Xu, Guixuan and Sun, Hao and Chen, Hao

Journal:

Cell Death \& Disease (2024): 740

Diamond-Like Carbon Depositing on the Surface of Polylactide Membrane for Prevention of Adhesion Formation During Tendon Repair

Authors:

Xiao, Yao and Tao, Zaijin and Ju, Yufeng and Huang, Xiaolu and Zhang, Xinshu and Liu, Xiaonan and Volotovski, Pavel A and Huang, Chao and Chen, Hongqi and Zhang, Yaozhong and others,

Journal:

Nano-Micro Letters (2024): 1--22

Mitochondrial Common Deletion Level in Adipose Tissue Is Not Associated with Obesity but Is Associated with a Structural Change in Triglycerides as Revealed by FTIR Spectroscopy

Authors:

Bahtiyarb, Ayda Y{\i}lmaza Nurten and Zengind, Ay{\c{c}}a Do{\u{g}}an Mollao{\u{g}}luc Kagan and Onur, Halit Eren Taskind Ayla Karimovae and Ulutine, Baykarae Turgut and Onarane, Ilhan

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs

Authors:

Orjuela-Sanchez P, Duggan E, Nolan J, Frangos JA, Carvalho LJ.

Journal:

Malar J (2012): 366

Association of degree and type of edema in posterior reversible encephalopathy syndrome with serum lactate dehydrogenase level: initial experience

Authors:

Gao B, Liu FL, Zhao B.

Journal:

Eur J Radiol (2012): 2844

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

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Page updated on September 25, 2025

Component A: Enzyme Probe	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: L-Lactate Standard	2.25 mg/vial