Name: Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit
Brand: AAT Bioquest
SKU: 13813
Price: 355 USD
Availability: InStock

Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit is a colorimetric assay kit for detecting L-LDH activity in biological and cell culture samples.

Colorimetric detection: Uses a chromogenic NADH sensor to measure L-LDH activity through an enzyme-coupled reaction.
High sensitivity: Detects L-LDH activity as low as 3 mU/mL in a 100 µL reaction volume.
L-LDH specific: Measures L-lactate dehydrogenase activity through proportional NADH generation.
Microplate compatible: Read the absorbance ratio at 575/605 nm using a clear-bottom microplate.
Applications: Suitable for L-LDH measurements in serum, plasma, urine, and cell culture samples.

L-LDH dose response was measured with Amplite® Colorimetric L-Lactate Dehydrogenase Assay Kit in a 96-well white wall/clear bottom plate using a SpectraMax Plus (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13813	200 Tests	Price

Overview

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyzes the interconversion of pyruvate and lactate. LDH is present in cytosol of a wide variety of organisms, including animals and plants. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. LDH is also elevated in certain pathological conditions such as cancer. This Amplite® Lactate Dehydrogenase Assay Kit provides a absoption-based method for detecting L-lactate dehydrogenase (L-LDH) in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the concentration of NADH that is specifically monitored by a chromogenic NADH sensor. This assays is specific for L-LDH. The absorption signal can be read by an absorption microplate reader an absorbance ratio of A575nm/A605nm. With this colorimetric Amplite® L-lactate Dehydrogenase Assay Kit, we were able to detect as little as 3 mU/mL L-lactate dehydrogenase in a 100 µL reaction volume.

Citations

View all 13 citations: Citation Explorer

The Role of the ArcA Metabolic Regulator in Gram-Negative Bacteremia

Authors:

Brown, Aric

METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression

Authors:

Xia, Peng and Zhang, Hao and Lu, Haofeng and Xu, Kequan and Jiang, Xiang and Jiang, Yuke and Gongye, Xiangdong and Chen, Zhang and Liu, Jie and Chen, Xi and others,

Journal:

Cancer Communications (2023)

Biophysical and Biochemical Characterization of TP0037, a d-Lactate Dehydrogenase, Supports an Acetogenic Energy Conservation Pathway in Treponema pallidum

Authors:

Deka, Ranjit K and Liu, Wei Z and Norgard, Michael V and Brautigam, Chad A

Journal:

Mbio (2020): e02249--20

Activation of Melatonin Receptor 2 But Not Melatonin Receptor 1 Mediates Melatonin-conferred Cardio-protection Against Myocardial Ischemia/Reperfusion Injury

Authors:

Han, Dong and Wang, Yong-Jun and Chen, Jiangwei and Zhang, Jibin and Yu, Peng and Zhang, Ran and Li, Shuang and Tao, Bo and Wang, Yabin and Qiu, Ya and others, undefined

Journal:

Journal of pineal research (2019): e12571

Mechanisms behind resistance to PI3K inhibitor treatment induced by the PIM kinase

Authors:

Song, Jin H and Singh, Neha and Luevano, Libia A and Padi, Sathish KR and Okumura, Koichi and Olive, Virginie and Black, Stephen M and Warfel, Noel A and Goodrich, David W and Kraft, Andrew S

Journal:

Molecular cancer therapeutics (2018): 2710--2721

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Serum lactate dehydrogenase predicts for overall survival benefit in patients with metastatic renal cell carcinoma treated with inhibition of mammalian target of rapamycin

Authors:

Armstrong AJ, George DJ, Halabi S.

Journal:

J Clin Oncol (2012): 3402

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575/605 nm
Recommended plate	Clear bottom

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Page updated on October 3, 2026

Component A: Enzyme Mix	1 bottle (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: L-Lactate Dehydrogenase	10 U/vial