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Amplite® Colorimetric Lipase Activity Assay Kit
The Amplite® Colorimetric Lipase Activity Assay Kit offers a straightforward and efficient protocol for quantifying lipase activity. This kit utilizes a coupled enzymatic reaction, which converts the substrate into a colored product (586 nm) proportional to the lipase activity present. It is suitable for detecting lipase activity in a variety of biological samples, including cells, supernatant, tissue extracts, and serum. Lipases belong to a class of enzymes that catalyze the hydrolysis of ester bonds in lipids, such as triglycerides, phospholipids, and cholesterol esters. These enzymes are essential for the digestion, absorption, and metabolism of dietary fats in the body, thereby playing a crucial role in lipid metabolism and cell signaling. Lipases are produced by various organs and tissues, including the pancreas, liver, intestine, and adipose tissue. Monitoring lipase activity is critical in screening and diagnosis of diseases like Pancreatitis, Cystic fibrosis, Celiac disease, Crohn's disease, and Hyperlipidemia.
Lipase dose response was measured with the Amplite® Colorimetric Lipase Activity Assay Kit in a 96-well white/clear bottom plate using a SpectraMax microplate reader (Molecular devices).
Lipase dose response was measured with the Amplite® Colorimetric Lipase Activity Assay Kit in a 96-well white/clear bottom plate using a SpectraMax microplate reader (Molecular devices).
CatalogSize
Price
Quantity
11315100 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
UNSPSC12352200
Instrument settings

Absorbance microplate reader
Absorbance586 nm
Recommended plateBlack or white plate with clear bottom
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Page updated on September 23, 2025