Name: Amplite® Colorimetric Lipase Activity Assay Kit
Brand: AAT Bioquest
SKU: 11315
Price: 516 USD
Availability: InStock

Amplite® Colorimetric Lipase Activity Assay Kit

Amplite® Colorimetric Lipase Activity Assay Kit is a colorimetric assay kit for quantifying lipase activity in biological samples through a coupled enzymatic readout.

Colorimetric detection: Uses a coupled enzymatic reaction to generate a colored product proportional to lipase activity.
Standard range: Supports lipase standards from 0.625 to 40 µg/mL.
Rapid workflow: Measures lipase activity after a 10–30 minute incubation at 37 °C.
Microplate compatible: Read absorbance at 586 nm using a black or white clear-bottom microplate.
Applications: Suitable for lipase activity measurements in cells, supernatants, tissue extracts, and serum.

Lipase dose response was measured with the Amplite® Colorimetric Lipase Activity Assay Kit in a 96-well white/clear bottom plate using a SpectraMax microplate reader (Molecular devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11315	100 Tests	Price

Overview

The Amplite® Colorimetric Lipase Activity Assay Kit offers a straightforward and efficient protocol for quantifying lipase activity. This kit utilizes a coupled enzymatic reaction, which converts the substrate into a colored product (586 nm) proportional to the lipase activity present. It is suitable for detecting lipase activity in a variety of biological samples, including cells, supernatant, tissue extracts, and serum. Lipases belong to a class of enzymes that catalyze the hydrolysis of ester bonds in lipids, such as triglycerides, phospholipids, and cholesterol esters. These enzymes are essential for the digestion, absorption, and metabolism of dietary fats in the body, thereby playing a crucial role in lipid metabolism and cell signaling. Lipases are produced by various organs and tissues, including the pancreas, liver, intestine, and adipose tissue. Monitoring lipase activity is critical in screening and diagnosis of diseases like Pancreatitis, Cystic fibrosis, Celiac disease, Crohn's disease, and Hyperlipidemia.

References

View all 50 references: Citation Explorer

Nanodiamonds and natural deep eutectic solvents as potential carriers for lipase.

Authors:

Putra, Sharifah Shahira Syed and Chew, Chia Yong and Hayyan, Adeeb and Elgharbawy, Amal A M and Taskin-Tok, Tugba and Hayyan, Maan and Ngoh, Gek Cheng and Saleh, Jehad and Al Abdulmonem, Waleed and Alghsham, Ruqaih S and Nor, Mohd Roslan Mohd and Aldaihani, Ahmad G H and Basirun, Wan Jefrey

Journal:

International journal of biological macromolecules (2024): 132245

A nutrient responsive lipase mediates gut-brain communication to regulate insulin secretion in Drosophila.

Authors:

Singh, Alka and Abhilasha, Kandahalli Venkataranganayaka and Acharya, Kathya R and Liu, Haibo and Nirala, Niraj K and Parthibane, Velayoudame and Kunduri, Govind and Abimannan, Thiruvaimozhi and Tantalla, Jacob and Zhu, Lihua Julie and Acharya, Jairaj K and Acharya, Usha R

Journal:

Nature communications (2024): 4410

Adipose Triglyceride Lipase-Mediated Adipocyte Lipolysis Exacerbates Acute Pancreatitis Severity in Mouse Models and Patients.

Authors:

Xie, Xiaochun and Liu, Yang and Yang, Qi and Ma, Xiaojie and Lu, Yingying and Hu, Yuepeng and Zhang, Guofu and Ke, Lu and Tong, Zhihui and Liu, Yuxiu and Xue, Jing and Lu, Guotao and Li, Weiqin

Journal:

The American journal of pathology (2024)

A comparative analysis of canine pancreatic lipase tests for diagnosing pancreatitis in dogs.

Authors:

Kim, Jin-Kyung and Hwang, Sun Young and Kim, Se Eun and Lee, Gahyun and Ji, Soungjin and Kim, Jungho and Kim, Yongbaek

Journal:

Journal of veterinary science (2024): e48

Assessment of the efficiency and stability of enzymatic membrane reaction utilizing lipase covalently immobilized on a functionalized hybrid membrane.

Authors:

Rezaie, Hosna and Kajani, Abolghasem Abbasi and Jafarian, Faranak and Asgari, Sina and Taheri-Kafrani, Asghar and Bordbar, Abdol-Khalegh

Journal:

Journal of biotechnology (2024): 23-31

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	586 nm
Recommended plate	Black or white plate with clear bottom

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Page updated on October 25, 2026

Component A: Lipase Assay Buffer	25 mL
Component B: Lipase Substrate Buffer	10 mL
Component C: Lipase Substrate	0.25 mL
Component D: Lipase Standard	1 Vial