Name: Amplite® Colorimetric Maleimide Quantitation Kit
Brand: AAT Bioquest
SKU: 5525
Price: 232 USD
Availability: InStock

Amplite® Colorimetric Maleimide Quantitation Kit

Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength. This colorimetric maleimide assay kit quantifies maleimide groups by first reacting a sample with a known amount of thiol present in excess and then assaying the remaining unreacted thiol using 4,4'-DTDP with a molar extinction coefficient of 19,800 M -1cm-1. The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups. This spectrophotometric assay for the determination of maleimide groups is a reverse GSH assay. It takes advantage of the high reactivity of thiols of GSH with the maleimide moiety. Maleimide of the sample is allowed to form a stable thiosuccinimidyl linkage with GSH. After the reaction of the sample is complete, the excess GSH, i.e., the remaining thiols of GSH in the reaction mixture, is estimated by using 4,4'-DTDP. The amount of GSH reacted with the sample is titrated to determine the extent of maleimide. For more sensitive maleimide quantitation, we recommend that you use our fluorimetric kit # 5523 that has higher sensitivity.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5525	100 tests	Price

Citations

View all 9 citations: Citation Explorer

Polysaccharide-conjugate vaccines for Burkholderia pseudomallei based on the O-antigens of serotypes A and B.

Authors:

Yan, Jingmin and Huang, Jinzhu and Lu, Xiaoxue and He, Xiaoyi and Rao, Chenglong and Nan, Dongqi and Lu, Haiyang and Guo, Linming and Li, Qian and Wang, Shiwei and others,

Journal:

Carbohydrate Polymer Technologies and Applications (2025): 100859

Perlecan-targeted nanoparticles for drug delivery to triple-negative breast cancer

Authors:

Khanna, Vidhi and Kalscheuer, Stephen and Kirtane, Ameya and Zhang, Wenqui and Panyam, Jayanth

Journal:

Future Drug Discovery (2019)

Conjugation with eight-arm PEG markedly improves the in vitro activity and prolongs the blood circulation of staphylokinase

Authors:

Qi, Fangbing and Hu, Chunyang and Yu, Weili and Hu, Tao

Journal:

Bioconjugate chemistry (2018)

Conjugation with an inulin-chitosan adjuvant markedly improves the immunogenicity of Mycobacterium tuberculosis CFP10-TB10. 4 fusion protein

Authors:

Yu, Weili and Hu, Tao

Journal:

Molecular Pharmaceutics (2016)

Successful acquisition of a neutralizing monoclonal antibody against a novel neutrophil-activating peptide, mitocryptide-1

Authors:

Hattori, Tatsuya and Nakashima, Kenta and Marutani, Takayuki and Kiso, Yoshiaki and Nishi, Yoshisuke and Mukai, Hidehito

Journal:

Biochemical and biophysical research communications (2015): 54--59

References

View all 38 references: Citation Explorer

Quantitation of microparticles released from coated-platelets

Authors:

Dale GL, Remenyi G, Friese P.

Journal:

J Thromb Haemost (2005): 2081

Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo3

Authors:

Chen J, Lu Z, Lawrence TS, Smith DE.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2005): 161

A new maleimide-bound acid-cleavable solid-support reagent for profiling phosphorylation

Authors:

Chowdhury SM, Munske GR, Siems WF, Bruce JE.

Journal:

Rapid Commun Mass Spectrom (2005): 899

Characterization of site-specific ScFv PEGylation for tumor-targeting pharmaceuticals

Authors:

Natarajan A, Xiong CY, Albrecht H, DeNardo GL, DeNardo SJ.

Journal:

Bioconjug Chem (2005): 113

Reactions of cysteines substituted in the amphipathic N-terminal tail of a bacterial potassium channel with hydrophilic and hydrophobic maleimides

Authors:

Li J, Xu Q, Cortes DM, Perozo E, Laskey A, Karlin A.

Journal:

Proc Natl Acad Sci U S A (2002): 11605

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	324 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
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Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 5, 2025

Component A: MEA	1 vial
Component B: 4,4'-DTDP	1 vial
Component C: Assay Buffer	1 bottle (50 mL)
Component D: DMSO	1 vial (1 mL)