Name: Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit
Brand: AAT Bioquest
SKU: 10070
Price: 415 USD
Availability: InStock

Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit

Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit is a colorimetric assay kit for quantifying MDA as a marker of lipid peroxidation and oxidative stress.

Colorimetric detection: Uses MDA Blue™ to generate a blue product proportional to MDA concentration.
High specificity: Measures MDA with little interference from other aldehydes.
Rapid workflow: Quantifies MDA without the heating steps required for traditional TBARS assays.
Microplate compatible: Read absorbance at 695 nm using a clear-bottom plate.
Applications: Suitable for assessing lipid peroxidation and oxidative stress in biological samples.

Protocol

SDS

Catalog	Size	Price	Quantity
10070	200 Tests	Price

Overview

Malondialdehyde (MDA) is natural byproduct of lipid peroxidation and is widely used as a key indicator to determine the oxidative stress and free radical formation. Measurement of MDA has historically relied on the reaction with thiobarbituric acid (TBA) to results in a compound that can be measured colorimetrically at or fluorimetrically. But this assay is not specific to MDA and also takes place under acidic conditions at 90-100 °C. There have been a number of commercial ELISA kits, which makes it more expensive and tedious. The Amplite® Colorimetric Malondialdehyde (MDA) Quantitation Kit offers the most rapid and convenient method to measure MDA without the TBARS heating steps. MDA Blue™ reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate reader. This assay is very fast and specific to MDA with little interference from other aldehydes.

Citations

View all 2 citations: Citation Explorer

Hydrogen-generating Si-based agent protects against skin flap ischemia--reperfusion injury in rats

Authors:

Otani, Naoya and Tomita, Koichi and Kobayashi, Yuki and Kuroda, Kazuya and Koyama, Yoshihisa and Kobayashi, Hikaru and Kubo, Tateki

Journal:

Scientific reports (2022): 1--8

Pre-treatment with curcumin ameliorates cisplatin-induced kidney damage by suppressing kidney inflammation and apoptosis in rats

Authors:

Soetikno, Vivian and Sari, Shinta Dewi Permata and Maknun, Lulu Ul and Sumbung, Nielda Kezia and Rahmi, Deliana Nur Ihsani and Pandhita, Bashar Adi Wahyu and Louisa, Melva and Estuningtyas, Ari

Journal:

Drug research (2019): 75--82

References

View all 21 references: Citation Explorer

Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges.

Authors:

Tsikas D., undefined

Journal:

Anal Biochem (2016)

Formation of Malondialdehyde, 4-Hydroxynonenal, and 4-Hydroxyhexenal during in Vitro Digestion of Cooked Beef, Pork, Chicken, and Salmon

Authors:

Steppeler C, Haugen JE, Rodbotten R, Kirkhus B.

Journal:

J Agric Food Chem (2016): 487

Development, validation and biomedical applications of stable-isotope dilution GC-MS and GC-MS/MS techniques for circulating malondialdehyde (MDA) after pentafluorobenzyl bromide derivatization: MDA as a biomarker of oxidative stress and its relation to 15

Authors:

Tsikas D, Rothmann S, Schneider JY, Suchy MT, Trettin A, Modun D, Stuke N, Maassen N, Frolich JC.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2016): 95

Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise

Authors:

Spirl, undefined and eli AL, Deminice R, Jordao AA.

Journal:

Int J Sports Med (2014): 14

A specific, accurate, and sensitive measure of total plasma malondialdehyde by HPLC

Authors:

Moselhy HF, Reid RG, Yousef S, Boyle SP.

Journal:

J Lipid Res (2013): 852

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	695 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: MDA Blue™	1 vial
Component B: Dilution Buffer	1 bottle (10 mL)
Component C: MDA Standard	1 vial (lyophilized powder)
Component D: Reaction Solution	1 bottle (10 mL)