Amplite® Colorimetric NADP/NADPH Ratio Assay Kit

Amplite® Colorimetric NADP/NADPH Ratio Assay Kit is a colorimetric assay kit for measuring NADP, NADPH, and the NADP/NADPH ratio in biological samples.

Colorimetric detection: Uses a chromogenic NADPH probe with absorbance at approximately 460 nm.
High sensitivity: Detects as little as 3 µM NADPH in a 100 µL assay volume.
Ratio measurement: Uses extraction and neutralization reagents to enable separate measurement of NADP and NADPH.
Microplate compatible: Supports 96-well or 384-well formats with absorbance detection at approximately 460 nm.
Applications: Suitable for NADP/NADPH quantitation and redox-status measurements in biological samples.

Amplite® Colorimetric NADP/NADPH Ratio Assay Kit is used to measure total NADP/NADPH amount in a white/clear 96-well microplate using a SpectraMax microplate reader (Molecular devices).

Protocol

SDS

Catalog	Size	Price	Quantity
15274	250 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. Our Amplite® NADP/NADPH Ratio Assay Kit provides a convenient method for sensitive detection of NADP, NADPH and their ratio. The NADPH probe is a chromogenic sensor that has its maximum absorbance at ~460 nm upon NADH reduction. The absorbance increase at ~460 nm is directly proportional to the concentration of NADPH in the solution. The NADPH probe can recognize NADPH in an enzyme-free reaction, and the signal can be easily read by an absorbance microplate reader at ~460 nm. The Amplite® Colorimetric NADPH Assay Kit provides a sensitive assay to detect as little as 3 µM NADPH in a 100 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Citations

View all 75 citations: Citation Explorer

Acetylation-modified phosphoglycerate dehydrogenase is associated with enhanced serine biosynthesis and one-carbon metabolism, supporting heat resistance in Pleurotus ostreatus

Authors:

Hu, Yanru and Zheng, Xiukun and Du, Rui and Liu, Qing and Chen, Zhaoyang and Chen, Yujie and Qi, Yuan Cheng and Wang, Fengqin and Wen, Qing and Shen, Jinwen

Journal:

International Journal of Biological Macromolecules (2026): 153200

Systematic Engineering To Enhance Citronellol Production in Yeast

Authors:

Gao, Qi and Wang, Herong and Shan, Mengying and Wu, Fangyuan and Jiang, Guozhen and Yao, Mingdong and Wang, Ying and Xiao, Wenhai and Yuan, Ying-Jin

Journal:

Journal of Agricultural and Food Chemistry (2025)

Systematic metabolic engineering enables highly efficient production of vitamin A in Saccharomyces cerevisiae

Authors:

Shi, Yi and Lu, Shuhuan and Zhou, Xiao and Wang, Xinhui and Zhang, Chenglong and Wu, Nan and Dong, Tianyu and Xing, Shilong and Wang, Ying and Xiao, Wenhai and others,

Journal:

Synthetic and Systems Biotechnology (2024)

Synergistic and stepwise treatment of resveratrol and catechol in Haematococcus pluvialis for the overproduction of biomass and astaxanthin

Authors:

Qiu, Jia-Fan and Yang, Yu-Cheng and Li, Ruo-Yu and Jiao, Yu-Hu and Mou, Jin-Hua and Yang, Wei-Dong and Lin, Carol Sze Ki and Li, Hong-Ye and Wang, Xiang

Journal:

Biotechnology for Biofuels and Bioproducts (2024): 80

The Function of HDAC6 Mediated Prx2 Acetylation in Neuronal Apoptosis Related Alzheimer's disease

Authors:

Zhang, Lijie and Hu, Jinxia and Zhang, Tao and Gao, Huimin and Wu, Xingrui and Zhang, Conghui and Zhang, Cheng and Chen, Hao and Yang, Minggang and Cao, Xichuan and others,

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	460 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: NADP/NADPH Recycling Enzyme Mix	2 bottles (lyophilized powder)
Component B-I: NADPH Probe	1 bottle (4 mL)
Component B-II: NADPH Probe Buffer	1 bottle (16 mL)
Component C: NADPH Standard	1 vial (167 µg)
Component D: NADP Extraction Solution	1 bottle (10 mL)
Component E: Neutralization Solution	1 bottle (10 mL)
Component F: Extraction Control Solution	1 bottle (10 mL)
Component G: Lysis Buffer	1 bottle (10 mL)