Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*

Amplite® Colorimetric Oxaloacetate Assay Kit

Red Color

Amplite® Colorimetric Oxaloacetate Assay Kit Red Color is a colorimetric assay kit for quantifying oxaloacetate in biological samples through an enzyme-coupled hydrogen peroxide readout.

Colorimetric detection: Uses Amplite® Red to monitor hydrogen peroxide generated from oxaloacetate at 575 nm.
Standard range: Supports oxaloacetate standards from 5 to 320 µM.
Enzyme-coupled chemistry: Converts oxaloacetate to pyruvate and generates a proportional hydrogen peroxide signal.
Microplate compatible: Supports 96-well or 384-well formats.
Applications: Suitable for oxaloacetate quantitation in metabolic and biochemical research samples.

Oxaloacetate dose response was measured with the Amplite® Colorimetric Oxaloacetate Assay Kit on a clear bottom 96-well plate using a SpectraMax microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13840	200 Tests	Price

Overview

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate. It is also involved in gluconeogenesis, urea cycle, glyoxylate cycle, amino acid synthesis, and fatty acid synthesis. The lack of oxaloacetate limits gluconeogenesis and urea cycle function, and can lead to decreased production of energy. Oxaloacetate can be also used as blood glutamate scavengers to provide neuroprotection after traumatic brain injury, expressed both by reduced neuronal loss in the hippocampus and improved neurologic outcomes. Amplite® Colorimetric Oxaloacetate Assay Kit offers a sensitive assay for quantifying oxaloacetate in biological samples. Oxaloacetate is converted to pyruvate that generates hydrogen peroxide through an enzyme coupled reaction. The production of hydrogen peroxide is monitored with Amplite® Red by an absorbance microplate reader at 575 nm.

References

View all 17 references: Citation Explorer

Enzymatic assay for D-aspartic acid using D-aspartate oxidase and oxaloacetate decarboxylase

Authors:

Kato S, Ikuta T, Hemmi H, Takahashi S, Kera Y, Yoshimura T.

Journal:

Biosci Biotechnol Biochem (2012): 2150

Assay of blood and tissue oxaloacetate and alpha-ketoglutarate by isotope dilution gas chromatography-mass spectrometry

Authors:

Laplante A, Comte B, Des Rosiers C.

Journal:

Anal Biochem (1995): 580

Effect of oxaloacetate and phosphorylation on ATP-citrate lyase activity

Authors:

Pentyala SN, Benjamin WB.

Journal:

Biochemistry (1995): 10961

Novel oxaloacetate effect on mitochondrial Ca2+ movement

Authors:

Leikin YN, Zharova TV, Tjulina OV.

Journal:

FEBS Lett (1993): 35

A sensitive multienzymatic assay for the measurement of pyruvate, dihydroxyacetone phosphate, oxaloacetate, and acetoacetate in clear extracts from biological samples

Authors:

Arias-Mendoza F, Pina E.

Journal:

Prep Biochem (1991): 211

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (20 mL)
Component D: Oxaloacetate Decarboxcylase (OAC)	2 vials (lyophilized powder)
Component E: Oxaloacetate Standard	1 vial (lyophilized powder)
Component F: DMSO	1 vial (100 µL)