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Amplite® Colorimetric Oxaloacetate Assay Kit
Red Color
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate. It is also involved in gluconeogenesis, urea cycle, glyoxylate cycle, amino acid synthesis, and fatty acid synthesis. The lack of oxaloacetate limits gluconeogenesis and urea cycle function, and can lead to decreased production of energy. Oxaloacetate can be also used as blood glutamate scavengers to provide neuroprotection after traumatic brain injury, expressed both by reduced neuronal loss in the hippocampus and improved neurologic outcomes. Amplite® Colorimetric Oxaloacetate Assay Kit offers a sensitive assay for quantifying oxaloacetate in biological samples. Oxaloacetate is converted to pyruvate that generates hydrogen peroxide through an enzyme coupled reaction. The production of hydrogen peroxide is monitored with Amplite® Red by an absorbance microplate reader at 575 nm.
Oxaloacetate dose response was measured with the Amplite® Colorimetric Oxaloacetate Assay Kit on a clear bottom 96-well plate using a SpectraMax microplate reader (Molecular Devices).
Oxaloacetate dose response was measured with the Amplite® Colorimetric Oxaloacetate Assay Kit on a clear bottom 96-well plate using a SpectraMax microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
13840200 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance575 nm
Recommended plateClear bottom
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Page updated on October 13, 2025