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Amplite® Colorimetric Peroxidase (HRP) Assay Kit
Blue Color
Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. This kit uses Amplite® Blue, our ultrasensitive chromogenic HRP substrate. Our Amplite® Blue is a chromogenic peroxidase substrate that is much more sensitive to both H2O2 and peroxidase than other chromogenic peroxidase substrates such as TMB, ABTS, OPD and K-Blue. Amplite® Blue generates a highly absorptive material that has maximum absorption of 664 nm. This near infrared absorption minimizes the background absorption that is often caused by the autoabsorption of biological samples that rarely absorb light beyond 600 nm. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. The kit provides an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
HRP dose response was measured with Amplite® Colorimetric Peroxidase Assay Kit in a white wall/clear bottom 96-well plate using a NovoStar absorbance microplate reader (BMG Labtech).
HRP dose response was measured with Amplite® Colorimetric Peroxidase Assay Kit in a white wall/clear bottom 96-well plate using a NovoStar absorbance microplate reader (BMG Labtech).
CatalogSize
Price
Quantity
11551500 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Absorbance microplate reader
Absorbance664 ± 5 nm
Recommended plateClear bottom
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Page updated on October 13, 2025