Name: Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
Brand: AAT Bioquest
SKU: 11551
Price: 237 USD
Availability: InStock

Amplite® Colorimetric Peroxidase (HRP) Assay Kit

Blue Color

Amplite® Colorimetric Peroxidase (HRP) Assay Kit Blue Color is a colorimetric assay kit for detecting and quantifying horseradish peroxidase activity in a microplate readout.

Colorimetric detection: Uses Amplite® Blue to generate a highly absorptive product.
High sensitivity: Amplite® Blue provides greater sensitivity to HRP and hydrogen peroxide than common chromogenic substrates.
Reduced background: Near-infrared absorbance minimizes interference from biological sample autoabsorption.
Microplate compatible: Read absorbance at 664 ± 5 nm using a clear-bottom microplate.
Applications: Suitable for ELISAs, enzyme kinetics, and high-throughput screening of oxidase inhibitors.

HRP dose response was measured with Amplite® Colorimetric Peroxidase Assay Kit in a white wall/clear bottom 96-well plate using a NovoStar absorbance microplate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
11551	500 Tests	Price

Overview

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. Peroxidase is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques and Northern, Southern and Western blot analyses. We offer this quick (10 min) HRP assay in a one-step, homogeneous, no wash assay system. This kit uses Amplite® Blue, our ultrasensitive chromogenic HRP substrate. Our Amplite® Blue is a chromogenic peroxidase substrate that is much more sensitive to both H2O2 and peroxidase than other chromogenic peroxidase substrates such as TMB, ABTS, OPD and K-Blue. Amplite® Blue generates a highly absorptive material that has maximum absorption of 664 nm. This near infrared absorption minimizes the background absorption that is often caused by the autoabsorption of biological samples that rarely absorb light beyond 600 nm. The kit can be used for ELISAs, characterizing kinetics of enzyme reaction and high throughput screening of oxidase inhibitors, etc. The kit provides an optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

References

View all 49 references: Citation Explorer

Horseradish peroxidase-driven fluorescent labeling of nanotubes with quantum dots

Authors:

Didenko VV, Baskin DS.

Journal:

Biotechniques (2006): 295

Enzymatic oxidation of dipyridamole in homogeneous and micellar solutions in the horseradish peroxidase-hydrogen peroxide system

Authors:

Almeida LE, Imasato H, Tabak M.

Journal:

Biochim Biophys Acta (2006): 216

Recent advances in catalytic peroxidase histochemistry

Authors:

Krieg R, Halbhuber KJ.

Journal:

Cell Mol Biol (Noisy-le-grand) (2003): 547

Vesicular transport route of horseradish C1a peroxidase is regulated by N- and C-terminal propeptides in tobacco cells

Authors:

Matsui T, Nakayama H, Yoshida K, Shinmyo A.

Journal:

Appl Microbiol Biotechnol (2003): 517

Synthesis and purification of horseradish peroxidase-labeled oligonucleotides for tyramide-based fluorescence in situ hybridization

Authors:

van Gijlswijk RP, van de Corput MP, Bezrookove V, Wiegant J, Tanke HJ, Raap AK.

Journal:

Histochem Cell Biol (2000): 175

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	664 ± 5 nm
Recommended plate	Clear bottom

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Page updated on October 2, 2026

Component A: Amplite® Blue Peroxidase Substrate	1 vial
Component B: H2O2	1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer	1 bottle (100 mL)
Component D: Horseradish Peroxidase	1 vial (20 units)
Component E: DMSO	1 vial (0.5 mL)