Name: Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
Brand: AAT Bioquest
SKU: 13620
Price: 237 USD
Availability: InStock

Amplite® Colorimetric Sphingomyelinase Assay Kit

Blue Color

Amplite® Colorimetric Sphingomyelinase Assay Kit Blue Color is a colorimetric assay kit for quantifying neutral sphingomyelinase activity in biological samples.

Detection Principle: Detects phosphocholine generated from sphingomyelin hydrolysis using Amplite™ UltraBlue.
Instrument Compatibility: Provides an absorbance readout at 655 nm using a microplate reader.
Workflow: Uses a simple mix-and-read format without separation steps.
Applications: Suitable for biological samples and sphingomyelinase inhibitor screening.

Sphingomyelinase dose response was measured on a 96-well white wall/clear bottom plate with Amplite® Colorimetric Sphingomyelinase Assay Kit using a SpectraMax microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13620	200 Tests	Price

Overview

Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide. Activation of SMases in cells plays an important role in the cellular responses. Five types of sphingomyelinase (SMase) have been identified based on their cation dependence and pH optima of action. They are lysosomal acid SMase, secreted zinc-dependent acid SMase, magnesium-dependent neutral SMase, magnesium-independent neutral SMase, and alkaline SMase. Among the five types, the lysosomal acidic SMase and the magnesium-dependent neutral SMase are considered major candidates for the production of ceramide in the cellular response to stress. Our Amplite® Colorimetric Sphingomyelinase Assay Kit provides a sensitive method for detecting neutral SMase activity or screening its inhibitors. The kit uses Amplite® UltraBlue as a colorimetric probe to indirectly quantify the phosphocholine produced from the hydrolysis of sphingomyelin (SM) by sphingomyelinase (SMase). It can be used for measuring the SMase activity in blood, cell extracts or other solutions. The absorbance of light at 655 nm is proportional to the formation of phosphocholine, therefore to the SMase activity. The kit is an optimized "mix and read" assay that is compatible with HTS liquid handling instruments.

Citations

View all 7 citations: Citation Explorer

A study on the targeting of ceramide metabolism by (-)-epicatechin gallate, catechin and quercetin in A-549 lung cancer cell line

Authors:

Mashhadi Akbar Boojar, Masoud

Journal:

Nova Biologica Reperta (2019): 275--283

Doxepin mitigates noise induced neuronal damage in primary auditory cortex of mice via suppression of acid sphingomyelinase/ceramide pathway

Authors:

Su, Yu-Ting and Meng, Xing-Xing and Zhang, Xi and Guo, Yi-Bin and Zhang, Hai-Jun and Cheng, Yao-Ping and Xie, Xiao-Ping and Chang, Yao-Ming and Bao, Jun-Xiang

Journal:

The Anatomical Record (2017)

Riccardin DN induces lysosomal membrane permeabilization by inhibiting acid sphingomyelinase and interfering with sphingomyelin metabolism in vivo

Authors:

Li, Lin and Niu, Huanmin and Sun, Bin and Xiao, Yanan and Li, Wei and Yuan, Huiqing and Lou, Hongxiang

Journal:

Toxicology and Applied Pharmacology (2016): 175--184

New Aspects of Silibinin Stereoisomers and their 3-O-galloyl Derivatives on Cytotoxicity and Ceramide Metabolism in Hep G2 hepatocarcinoma Cell Line

Authors:

Mashhadi Akbar Boojar, Mahdi and Ejtemaei Mehr, Shahram and Hassanipour, Mahsa and Mashhadi Akbar Boojar, Masoud and Dehpour, Ahmad Reza

Journal:

Iranian Journal of Pharmaceutical Research (2016): 421--433

Mitochondrial respiration controls lysosomal function during inflammatory T cell responses

Authors:

Baixauli, Francesc and Acín-Pérez, Rebeca and Villarroya-Beltrí, Carolina and Mazzeo, Carla and Nunez-Andrade, Norman and Gab, undefined and é-Rodriguez, Enrique and Ledesma, Maria Dolores and Blázquez, Alberto and Martin, Miguel Angel and Falcón-Pérez, Juan Manuel and others, undefined

Journal:

Cell metabolism (2015): 485--498

References

View all 65 references: Citation Explorer

Alterations of myelin-specific proteins and sphingolipids characterize the brains of acid sphingomyelinase-deficient mice, an animal model of Niemann-Pick disease type A

Authors:

Buccinna B, Piccinini M, Prinetti A, Sc and roglio F, Prioni S, Valsecchi M, Votta B, Grifoni S, Lupino E, Ramondetti C, Schuchman EH, Giordana MT, Sonnino S, Rinaudo MT.

Journal:

J Neurochem (2009): 105

A novel sphingomyelinase-like enzyme in Ixodes scapularis tick saliva drives host CD4 T cells to express IL-4

Authors:

Alarcon-Chaidez FJ, Boppana VD, Hagymasi AT, Adler AJ, Wikel SK.

Journal:

Parasite Immunol (2009): 210

A novel mitochondrial sphingomyelinase in zebrafish cells

Authors:

Yabu T, Shimuzu A, Yamashita M.

Journal:

J Biol Chem (2009): 20349

A complex extracellular sphingomyelinase of Pseudomonas aeruginosa inhibits angiogenesis by selective cytotoxicity to endothelial cells

Authors:

Vasil ML, Stonehouse MJ, Vasil AI, Wadsworth SJ, Goldfine H, Bolcome RE, 3rd, Chan J.

Journal:

PLoS Pathog (2009): e1000420

Expression of alkaline sphingomyelinase in yeast cells and anti-inflammatory effects of the expressed enzyme in a rat colitis model

Authors:

Andersson D, Kotarsky K, Wu J, Agace W, Duan RD.

Journal:

Dig Dis Sci (2009): 1440

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	655 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 6, 2026

Component A: Enzyme Mix	2 bottles (lyophilized powder)
Component B: Sphingomyelin	1 vial (100 µL)
Component C: Amplite® UltraBlue	1 vial (lyophilized powder)
Component D: SMase Reaction Buffer	1 bottle (10 mL)
Component E: Assay Buffer	1 bottle (20 mL)
Component F: Sphingomyelinase Standard	1 vial (0.2 unit, lyophilized powder)
Component G: DMSO	1 vial (200 µL)