Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit

Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit is a colorimetric assay kit for quantifying SOD activity in solutions.

Detection Principle: Measures SOD-mediated inhibition of superoxide detection.
Sensitivity: Detects SOD activity down to 0.1 U/mL.
Reaction Chemistry: Uses xanthine oxidase-generated superoxide and ReadiView™ SOD560.
Instrument Compatibility: Absorbance detection at 560 nm using a microplate reader.
Plate Format: Compatible with 96- and 384-well microplates.

SOD dose response was measured with Amplite® Colorimetric Superoxide Dismutase Assay Kit in a 96-well white wall/clear bottom plate with a Spectrum Max microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11305	200 Tests	Price

Overview

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. Superoxide is one of the main reactive oxygen species in cells. It is a substantial contributor of pathology associated with neurodegenerative diseases, ischemia reperfusion injury, atherosclerosis and aging. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. In fact, mice lacking SOD1 develop a wide range of pathologies, including hepatocellular carcinoma, an acceleration of age-related muscle mass loss, an earlier incidence of cataracts and a reduced lifespan. Overexpression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation. The Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit provides a quick and sensitive method for the measurement of SOD activity in solutions. In the assay, xanthine is converted to superoxide radical ions, uric acid and hydrogen peroxide by xanthine oxidase (XO). Superoxide reacts with ReadiView™ SOD560 to generate a product that absorbs around 560 nm. SOD inhibits the reaction of ReadiView™ SOD560 with superoxide, thus reduces the absorption at 560 nm. The reduction in the absorption of ReadiView™ SOD560 at 560 nm is proportional to SOD activity. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format.

Citations

View all 11 citations: Citation Explorer

Antioxidant Properties of a Rhodiola rosea Constituent, Rosavin

Authors:

Brink, Drew F and Sapp, Tiara L and Ghafoor, Trwska S and Boyland, Paige A and Tamazawa, Yukako C and Kaur, Guneet and Shults, Nataliia V and Sullivan, Robert D and Suzuki, Yuichiro J

Journal:

Life (2026): 1510

Exploring Nutritional Evaluation of Cauliflower (Brassica oleracea var. Botrytis) Waste on the Thyroid Hormone, Antioxidant Status, Immunity and Expression of Interleukin 6 and HSP 70 Genes in Broilers

Authors:

Mahesh, Theerthesh and Biswas, Avishek and Kolluri, Gautham and Saha, Subodh Kumar and Bansod, Ashvini Pundalik and Khandare, Rajeshwar

Journal:

Waste and Biomass Valorization (2026): 1--15

Erinacine A-Enriched Hericium erinaceus Mycelium Ethanol Extract Lessens Cellular Damage in Cell and Drosophila Models of Spinocerebellar Ataxia Type 3 by Improvement of Nrf2 Activation

Authors:

Wu, Yu-Ling and Sun, Hai-Lun and Chang, Jui-Chih and Lin, Wei-Yong and Chen, Pei-Yin and Chen, Chin-Chu and Lee, Li-Ya and Li, Chien-Chun and Hsieh, Mingli and Chen, Haw-Wen and others,

Journal:

Antioxidants (2024): 1495

Intracellular pyruvate as one of the major bioactive substances of lactic acid bacteria isolated from kimchi

Authors:

Kang, Jin Yong and Lee, Moeun and Song, Jung Hee and Choi, Eun Ji and Mun, So Yeong and Kim, Daun and Lim, Seul Ki and Kim, Namhee and Park, Bo Yeon and Chang, Ji Yoon

Journal:

Journal of Food Science (2024)

Astragaloside IV Reduces Mutant Ataxin-3 Levels and Supports Mitochondrial Function in Spinocerebellar Ataxia Type 3

Authors:

Lin, Yongshiou and Cheng, Wenling and Chang, Juichih and Wu, Yuling and Hsieh, Mingli and Liu, Chin-San

References

View all 111 references: Citation Explorer

Functional polymorphism in manganese superoxide dismutase and antioxidant status: their interactions on the risk of cervical intraepithelial neoplasia and cervical cancer

Authors:

Tong SY, Lee JM, Song ES, Lee KB, Kim MK, Lee JK, Son SK, Lee JP, Kim JH, Kwon YI.

Journal:

Gynecol Oncol (2009): 272

Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses

Authors:

Sanchez-Venegas JR, Dinamarca J, Moraga AG, Gidekel M.

Journal:

BMC Res Notes (2009): 198

Imidazolate-bridged dicopper(II) and copper(II)-zinc(II) complexes of macrocyclic ligand with methylimidazol pendants: Model study of copper(II)-zinc(II) superoxide dismutase

Authors:

Yuan Q, Cai K, Qi ZP, Bai ZS, Su Z, Sun WY.

Journal:

J Inorg Biochem (2009): 1156

Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase

Authors:

Lin CT, Tseng WC, Hsiao NW, Chang HH, Ken CF.

Journal:

Fish Shellfish Immunol (2009): 318

Superoxide dismutase activity of corrole metal complexes

Authors:

Eckshtain M, Zilbermann I, Mahammed A, Saltsman I, Okun Z, Maimon E, Cohen H, Meyerstein D, Gross Z.

Journal:

Dalton Trans (2009): 7879

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	560 nm
Recommended plate	Clear bottom

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Fax
Email	sales@aatbio.com
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Page updated on October 8, 2026

Component A: ReadiView™ SOD560	2 Bottles
Component B: 50X Xanthine	1 Vial (100 µL)
Component C: Xanthine Oxidase	2 Vials
Component D: SOD Standard	1 Vial (500 Units)
Component E: Assay Buffer	1 Bottle (20 mL)