Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. Superoxide is one of the main reactive oxygen species in cells. It is a substantial contributor of pathology associated with neurodegenerative diseases, ischemia reperfusion injury, atherosclerosis and aging. SODs are an important antioxidant defense in nearly all cells exposed to superoxide radicals. In fact, mice lacking SOD1 develop a wide range of pathologies, including hepatocellular carcinoma, an acceleration of age-related muscle mass loss, an earlier incidence of cataracts and a reduced lifespan. Overexpression of SOD protects murine fibrosarcoma cells from apoptosis and promotes cell differentiation. The Amplite® Colorimetric Superoxide Dismutase (SOD) Assay Kit provides a quick and sensitive method for the measurement of SOD activity in solutions. In the assay, xanthine is converted to superoxide radical ions, uric acid and hydrogen peroxide by xanthine oxidase (XO). Superoxide reacts with ReadiView™ SOD560 to generate a product that absorbs around 560 nm. SOD inhibits the reaction of ReadiView™ SOD560 with superoxide, thus reduces the absorption at 560 nm. The reduction in the absorption of ReadiView™ SOD560 at 560 nm is proportional to SOD activity. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format.

SOD dose response was measured with Amplite® Colorimetric Superoxide Dismutase Assay Kit in a 96-well white wall/clear bottom plate with a Spectrum Max microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11305	200 Tests	Price

Citations

View all 9 citations: Citation Explorer

Erinacine A-Enriched Hericium erinaceus Mycelium Ethanol Extract Lessens Cellular Damage in Cell and Drosophila Models of Spinocerebellar Ataxia Type 3 by Improvement of Nrf2 Activation

Authors:

Wu, Yu-Ling and Sun, Hai-Lun and Chang, Jui-Chih and Lin, Wei-Yong and Chen, Pei-Yin and Chen, Chin-Chu and Lee, Li-Ya and Li, Chien-Chun and Hsieh, Mingli and Chen, Haw-Wen and others,

Journal:

Antioxidants (2024): 1495

Intracellular pyruvate as one of the major bioactive substances of lactic acid bacteria isolated from kimchi

Authors:

Kang, Jin Yong and Lee, Moeun and Song, Jung Hee and Choi, Eun Ji and Mun, So Yeong and Kim, Daun and Lim, Seul Ki and Kim, Namhee and Park, Bo Yeon and Chang, Ji Yoon

Journal:

Journal of Food Science (2024)

Astragaloside IV Reduces Mutant Ataxin-3 Levels and Supports Mitochondrial Function in Spinocerebellar Ataxia Type 3

Authors:

Lin, Yongshiou and Cheng, Wenling and Chang, Juichih and Wu, Yuling and Hsieh, Mingli and Liu, Chin-San

Amelioration of AlCl3-induced Memory Loss in the Rats by an Aqueous Extract of Guduchi, a Medhya Rasayana

Authors:

Jamadagni, Shrirang B and Ghadge, Pooja M and Tambe, Mukul S and Srinivasan, Marimuthu and Prasad, Goli Penchala and Jamadagni, Pallavi S and Prasad, Shyam Baboo and Pawar, Sharad D and Gurav, Arun M and Gaidhani, Sudesh N and others,

Journal:

Pharmacognosy Magazine (2023): 09731296221145063

Comparison of the Probiotic Potential between Lactiplantibacillus plantarum Isolated from Kimchi and Standard Probiotic Strains Isolated from Different Sources

Authors:

Jeong, Chang-Hee and Sohn, Hyejin and Hwang, Hyelyeon and Lee, Ho-Jae and Kim, Tae-Woon and Kim, Dong-Sub and Kim, Chun-Sung and Han, Sung-Gu and Hong, Sung-Wook

Journal:

Foods (2021): 2125

References

View all 111 references: Citation Explorer

Functional polymorphism in manganese superoxide dismutase and antioxidant status: their interactions on the risk of cervical intraepithelial neoplasia and cervical cancer

Authors:

Tong SY, Lee JM, Song ES, Lee KB, Kim MK, Lee JK, Son SK, Lee JP, Kim JH, Kwon YI.

Journal:

Gynecol Oncol (2009): 272

Molecular characterization of a cDNA encoding Cu/Zn superoxide dismutase from Deschampsia antarctica and its expression regulated by cold and UV stresses

Authors:

Sanchez-Venegas JR, Dinamarca J, Moraga AG, Gidekel M.

Journal:

BMC Res Notes (2009): 198

Imidazolate-bridged dicopper(II) and copper(II)-zinc(II) complexes of macrocyclic ligand with methylimidazol pendants: Model study of copper(II)-zinc(II) superoxide dismutase

Authors:

Yuan Q, Cai K, Qi ZP, Bai ZS, Su Z, Sun WY.

Journal:

J Inorg Biochem (2009): 1156

Characterization, molecular modelling and developmental expression of zebrafish manganese superoxide dismutase

Authors:

Lin CT, Tseng WC, Hsiao NW, Chang HH, Ken CF.

Journal:

Fish Shellfish Immunol (2009): 318

Superoxide dismutase activity of corrole metal complexes

Authors:

Eckshtain M, Zilbermann I, Mahammed A, Saltsman I, Okun Z, Maimon E, Cohen H, Meyerstein D, Gross Z.

Journal:

Dalton Trans (2009): 7879

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Absorbance microplate reader
Absorbance	560 nm
Recommended plate	Clear bottom

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Page updated on September 25, 2025

Component A: ReadiView™ SOD560	2 Bottles
Component B: 50X Xanthine	1 Vial (100 µL)
Component C: Xanthine Oxidase	2 Vials
Component D: SOD Standard	1 Vial (500 Units)
Component E: Assay Buffer	1 Bottle (20 mL)