Name: Amplite® Colorimetric Total NADP and NADPH Assay Kit
Brand: AAT Bioquest
SKU: 15260
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Total NADP and NADPH Assay Kit

Amplite® Colorimetric Total NADP and NADPH Assay Kit is a colorimetric assay kit for quantifying total NADP and NADPH in biological samples.

Detection Principle: Uses an enzyme cycling reaction for NADP/NADPH detection.
Signal Advantage: Provides greater sensitivity than direct UV absorbance at 340 nm.
Specificity: Enzymes specifically recognize NADP/NADPH.
Workflow: No NADP/NADPH purification is required.
Instrument Compatibility: Absorbance detection at 575 ± 5 nm using a microplate reader.

NADPH dose response was measured with Amplite® Colorimetric Total NADP and NADPH Assay Kit in a white/clear bottom 96-well plate using a NOVOStar microplate reader (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
15260	400 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite® NADP/NADPH Assay Kit provides a convenient method for sensitive detection of NADP and NADPH. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction. There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity.

Citations

View all 64 citations: Citation Explorer

Collateral lethality of NAD kinase in 1p36 deleted tumors drives vulnerability to NAD kinase 2 depletion

Authors:

Suyama-Banjo, Saki and Watanabe, Kengo and Ishikawa, Hirokazu and Yoshihama, Yohei and Hirota, Yasuhide and Tsutsumi, Shinji

Journal:

BBA Advances (2026): 100192

PGRN protects against serum deprivation-induced cell death by promoting the ROS scavenger system in cervical cancer

Authors:

Feng, Tingting and Xu, Xiaoying and Wang, Xiao and Tang, Wei and Lu, Yi

Journal:

Cell Death \& Disease (2024): 889

Synergistic and stepwise treatment of resveratrol and catechol in Haematococcus pluvialis for the overproduction of biomass and astaxanthin

Authors:

Qiu, Jia-Fan and Yang, Yu-Cheng and Li, Ruo-Yu and Jiao, Yu-Hu and Mou, Jin-Hua and Yang, Wei-Dong and Lin, Carol Sze Ki and Li, Hong-Ye and Wang, Xiang

Journal:

Biotechnology for Biofuels and Bioproducts (2024): 80

Enhancing the biotransformation efficiency of human CYP17A1 in Pichia pastoris by co-expressing CPR and glucose-6-phosphate dehydrogenase simultaneously

Authors:

Chen, Kexin and Liu, Chao and Shao, Minglong and Xu, Zhenghong and Yang, Taowei and Rao, Zhiming

Journal:

Systems Microbiology and Biomanufacturing (2021): 1--10

Effects of salt stress on low molecular antioxidants and redox state of plastoquinone and P700 in Arabidopsis thaliana (glycophyte) and Eutrema salsugineum (halophyte)

Authors:

Wiciarz, Monika and Niewiadomska, E and Kruk, Jerzy

Journal:

Photosynthetica (2018): 811--819

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	575 ± 5 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: NADP/NADPH Recycling Enzyme Mix	2 bottles (lyophilized powder)
Component B: NADPH Sensor Buffer	1 bottle (20 mL)
Component C: NADPH Standard	1 vial (167 µg)