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Amplite® Colorimetric Total NADP and NADPH Assay Kit
Enhanced Sensitivity
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. It forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is then used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis. The traditional NAD/NADH and NADP/NADPH assays are done by monitoring of NADH or NADPH absorption at 340 nm. This method suffers low sensitivity and high interference since the assay is done in the UV range that requires expensive quartz microplate. This Amplite® NADP/NADPH Assay Kit provides a convenient method for sensitive detection of NADP and NADPH. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction. There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Compared to Kit #15260, this kit has higher sensitivity.
NADPH dose response was measured with the Amplite® Colorimetric Total NADP and NADPH Assay Kit *Enhanced Sensitivity* in a 96-well white/clear bottom plate using a SpectraMax microplate reader (Molecular Devices).
NADPH dose response was measured with the Amplite® Colorimetric Total NADP and NADPH Assay Kit *Enhanced Sensitivity* in a 96-well white/clear bottom plate using a SpectraMax microplate reader (Molecular Devices).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
15276400 Tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Absorbance microplate reader
Absorbance460 nm
Recommended plateClear bottom
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Page updated on October 12, 2025