Amplite® Colorimetric Tyrosine Assay Kit

Product key features

Amplite® Colorimetric Tyrosine Assay Kit offers rapid and accurate quantification of tyrosine in complex biological samples.

Sensitive detection: Enables accurate measurement of low tyrosine concentrations
Fast workflow: One step, mix and read protocol with total assay time under 60 minutes
Broad applications: Suitable for studies in amino acid metabolism, enzymology, and nutritional biochemistry
Comparable alternative: Offers a reliable solution similar to Abcam’s tyrosine detection kits

Product description

Amplite® Colorimetric Tyrosine Assay Kit provides a sensitive and efficient method for quantifying tyrosine in biological and biochemical samples. The assay relies on a colorimetric reaction that generates a measurable chromogenic product proportional to tyrosine concentration, enabling reproducible analysis in research areas such as amino acid metabolism, enzymology, and nutritional science.

This assay follows a mix and read format and delivers results in under 60 minutes making it higly convienient for researchers. It supports high-throughput screening and is validated for sample types including plasma, serum, and tissue lysates. The kit includes a tyrosine calibration standard for precise quantification and minimizes interference from common biological substances, ensuring consistent performance across experimental conditions.

Example protocol

AT A GLANCE

Protocol Summary

Prepare and add standards and samples (50 µL)
Prepare and add Tyrosine working solutionto the standards and samples wells (50 µL)
Incubate the plate at room temperature for 30 to 60 minutes
Monitor the absorbance at 510 nm

Important: Bring all kit components to room temperature before starting the experiment.

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

Tyrosine standard solution (100 mM)

Add 100 µL Assay Buffer (Component B) to Tyrosine Standard (Component C) and mix well to prepare a 100 mM tyrosine standard solution.

Note: Store any unused Tyrosine stock solution at -20 °C.

Tyrosine Enzyme Stock Solution (25X)

Add 500 µL ddH₂O to Tyrosine Enzyme (Component A) and mix well to prepare a 50X Tyrosine Enzyme stock solutions

Note: Store any unused Tyrosine Enzyme stock solution at -20 °C.

PREPARATION OF STANDARD SOLUTIONS

For convenience, use the Serial Dilution Planner:
https://www.aatbio.com/tools/serial-dilution/11331

Preparation of Standard Curve

Add 10 µL of 100 mM Tyrosine Standard solution to 990 µL Tyrosine Assay Buffer (Component B) to prepare a 1 mM Tyrosine solution (Tyr7). Take 300 µL of the Tyr7 solution and perform 1:2 serial dilutions in the Tyrosine Assay Buffer (Component B) to prepare the remaining Tyrosine Standards (Tyr6 to Tyr1), resulting in a concentration range from 500 µM to 15.6 µM.

PREPARATION OF WORKING SOLUTION

Tyrosine working solution

Make a 1:50 dilution by adding 20 µL Tyrosine Enzyme Stock Solution (50X) to 1 mL of Tyrosine Assay Buffer (Component B) and mix well.

Note: Tyrosine working solution should be prepared immediately before use. We recommend using the working solution within 2 hours.

SAMPLE EXPERIMENTAL PROTOCOL

Table 1. Layout of Tyrosine standards and test samples in a clear bottom 96- wells microplate. Tyrosine standards (Tyr7-Tyr1 = 1000 µM to 15.6 µM), TS = Test Samples, BL = 0 µM Tyr.

Tyr7	Tyr7	TS	TS
Tyr6	Tyr6
Tyr5	Tyr5
Tyr4	Tyr4
Tyr3	Tyr3
Tyr2	Tyr2
Tyr1	Tyr1
BL	BL

The following protocol can be used as a guideline and should be optimized accordingly.

Prepare the standards and test samples according to the recommended protocol. Add 50 µL of each into the wells of a microplate.
Add 50 µL Tyrosine working solutionto each well containing standards and samples.
Incubate the reaction at room temperature for 30 to 60 minutes.
Monitor the absorbance with an absorbance plate reader at 510 nm.

References

View all 7 references: Citation Explorer

A robust high-throughput screening system to assess bacterial tyrosine ammonia lyase activity in the context of tyrosine inherited metabolic disorders.
Authors: Nulmans, Ine and Laga, Camille Annie and Salvi, Nina Stefanie and Desmet, Liesbeth and Lequeue, Sien and Neuckermans, Jessie and Schwaneberg, Ulrich and De Kock, Joery
Journal: Scientific reports (2024): 22175

Development of a clinical assay to measure chlorinated tyrosine in hair and tissue samples using a mouse chlorine inhalation exposure model.
Authors: Pantazides, Brooke G and Crow, Brian S and Quiñones-González, Jennifer and Perez, Jonas W and Harvilchuck, Jill A and Wallery, Jeffrey J and Hu, Tom C and Thomas, Jerry D and Johnson, Rudolph C and Blake, Thomas A
Journal: Analytical and bioanalytical chemistry (2021): 1765-1776

Evidence for the ectopic synthesis of melanin in human adipose tissue.
Authors: Randhawa, Manpreet and Huff, Tom and Valencia, Julio C and Younossi, Zobair and Chandhoke, Vikas and Hearing, Vincent J and Baranova, Ancha
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2009): 835-43

Inhibitory effects of progestogens on the estrogen stimulation of melanocytes in vitro.
Authors: Wiedemann, Christine and Nägele, Ursula and Schramm, Georg and Berking, Carola
Journal: Contraception (2009): 292-8

Tyrosinase activity in the sera of patients with malignant melanoma: method and specificity.
Authors: Nishioka, K and Romsdahl, M M and Fritsche, H A and McMurtrey, M J
Journal: International journal of cancer (1977): 689-93

Page updated on August 14, 2025

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