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Amplite® Colorimetric Tyrosine Assay Kit
Product key features
- High Sensitivity: Accurate detection of low tyrosine levels.
- Rapid Protocol: Results in under 60 minutes.
- Broad Compatibility: Works with plasma, serum, and tissue lysates.
- Reproducible: Minimizes interference for consistent outcomes.
- Versatile Applications: Ideal for metabolism, enzymology, and nutritional research.
Product description
The Amplite™ Colorimetric Tyrosine Assay Kit provides a sensitive and efficient method for quantifying tyrosine levels in biological and biochemical samples. The assay utilizes a colorimetric reaction to produce a chromogenic product proportional to tyrosine concentration, enabling reproducible measurements for applications in amino acid metabolism, enzymology, and nutritional science.
The assay features a straightforward mix-and-read protocol with a total assay time of less than 60 minutes, making it suitable for high-throughput applications. It is compatible with sample types such as plasma, serum, and tissue lysates and includes a tyrosine calibration standard to support accurate quantification. The assay is optimized to minimize interference from endogenous components, ensuring reliable performance.
Tyrosine is a non-essential aromatic amino acid with roles in neurotransmitter synthesis, thyroid hormone production, and melanin biosynthesis. Abnormal tyrosine levels are associated with metabolic disorders such as phenylketonuria, tyrosinemia, and albinism, making its accurate quantification critical for research in metabolic and physiological processes.
Example protocol
AT A GLANCE
Prepare and add standards and samples (50 µL)
Prepare and add Tyrosine working solution to the standards and samples wells (50 µL)
Incubate the plate at room temperature for 30 to 60 minutes
Monitor the absorbance at 510 nm
Important: Bring all kit components to room temperature before starting the experiment.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 100 µL Assay Buffer (Component B) to Tyrosine Standard (Component C) and mix well to prepare a 100 mM tyrosine standard solution.
Note: Store any unused Tyrosine stock solution at -20 °C.
Add 500 µL ddH2O to Tyrosine Enzyme (Component A) and mix well to prepare a 50X Tyrosine Enzyme stock solutions
Note: Store any unused Tyrosine Enzyme stock solution at -20 °C.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/11331
PREPARATION OF WORKING SOLUTION
Make a 1:50 dilution by adding 20 µL Tyrosine Enzyme Stock Solution (50X) to 1 mL of Tyrosine Assay Buffer (Component B) and mix well.
Note: Tyrosine working solution should be prepared immediately before use. We recommend using the working solution within 2 hours.
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. Layout of Tyrosine standards and test samples in a clear bottom 96- wells microplate. Tyrosine standards (Tyr7-Tyr1 = 1000 µM to 15.6 µM), TS = Test Samples, BL = 0 µM Tyr.
Tyr7 | Tyr7 | TS | TS | ||||
Tyr6 | Tyr6 | ||||||
Tyr5 | Tyr5 | ||||||
Tyr4 | Tyr4 | ||||||
Tyr3 | Tyr3 | ||||||
Tyr2 | Tyr2 | ||||||
Tyr1 | Tyr1 | ||||||
BL | BL |
The following protocol can be used as a guideline and should be optimized accordingly.
Prepare the standards and test samples according to the recommended protocol. Add 50 µL of each into the wells of a microplate.
Add 50 µL Tyrosine working solution to each well containing standards and samples.
Incubate the reaction at room temperature for 30 to 60 minutes.
Monitor the absorbance with an absorbance plate reader at 510 nm.
References
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Safety data sheet