Amplite® Colorimetric Urea Quantitation Kit *Blue Color*

Amplite® Colorimetric Urea Quantitation Kit

Blue Color

Amplite® Colorimetric Urea Quantitation Kit Blue Color is a colorimetric assay kit for quantifying urea in biological samples such as serum, plasma, and urine.

Detection Principle: Uses an enzyme-coupled reaction to produce a blue color signal.
Sensitivity: Detects urea down to 10 µM in 150 µL.
Signal Response: Color intensity is proportional to urea concentration.
Instrument Compatibility: Absorbance detection at 660–670 nm using a microplate reader.
Workflow: Compatible with 96- and 384-well plates without a separation step.

Urea dose response in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices) measured with Amplite® Colorimetric Urea Assay Kit.

Protocol

SDS

Catalog	Size	Price	Quantity
10058	200 Tests	Price

Overview

Urea is the final degradation product of protein and amino acid metabolism in animals. It is produced in liver, secreted by kidney and excreted through urine. The determination of urea is very useful test in clinical laboratory to monitor health status. The Blood Urea Nitrogen (BUN) test is a measure of the amount of nitrogen in the blood in the form of urea and is primarily used, along with the creatinine test to evaluate kidney function, helping diagnose kidney diseases. Our Amplite® Colorimetric Urea Assay Kit provides a simple and sensitive colorimetric method for the quantitation of urea concentration in biological samples such as serum, plasma and urine, etc. The assay is based on an enzyme-coupled reaction of urea in the assay buffer, and finally produces a blue colored product. The intensity of color produced is proportional to the concentration of urea in the sample, which can be measured colorimetrically at 660-670 nm. This Amplite® Colorimetric Urea Assay Kit provides a simple assay to detect as little as 10 µM urea in a 150 µL assay volume. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

References

View all 60 references: Citation Explorer

A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catalytic reaction and NH4+ associated particle reaction

Authors:

Liang A, Qin H, Zhou L, Zhang Y, Ouyang H, Wang P, Jiang Z.

Journal:

Bioprocess Biosyst Eng (2011): 639

Dimethyl formamide-free, urea-NaCl fluorescence in situ hybridization assay for Staphylococcus aureus

Authors:

Lawson TS, Connally RE, Vemulpad S, Piper JA.

Journal:

Lett Appl Microbiol. (2011)

Evaluation of diuron (3-[3,4-dichlorophenyl]-1,1-dimethyl urea) in a two-stage mouse skin carcinogenesis assay

Authors:

Ferrucio B, Franchi CA, Boldrin NF, de Oliveira ML, de Camargo JL.

Journal:

Toxicol Pathol (2010): 756

Sample prep for proteomics of breast cancer: proteomics and gene ontology reveal dramatic differences in protein solubilization preferences of radioimmunoprecipitation assay and urea lysis buffers

Authors:

Ngoka LC., undefined

Journal:

Proteome Sci (2008): 30

Improvement of the decision efficiency of the accuracy profile by means of a desirability function for analytical methods validation. Application to a diacetyl-monoxime colorimetric assay used for the determination of urea in transdermal iontophoretic extr

Authors:

Rozet E, Wascotte V, Lecouturier N, Preat V, Dewe W, Boulanger B, Hubert P.

Journal:

Anal Chim Acta (2007): 239

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	665 nm
Recommended plate	Clear bottom

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Page updated on October 4, 2026

Component A: Assay Enzyme Mix	1 vial (lyophilized powder)
Component B: Assay Buffer I	1 bottle (10 mL)
Component C: Assay Buffer II	1 bottle (10 mL)
Component D: Urea Standard	1 vial (1M, 100 µL)