Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit is a colorimetric assay kit for quantifying xanthine in biological samples.

Detection Principle: Detects hydrogen peroxide generated during xanthine oxidation.
Sensitivity: Detects xanthine down to 1.2 µM in a 100 µL reaction volume.
Reaction Chemistry: Uses xanthine oxidase and Amplite® Red.
Instrument Compatibility: Absorbance detection at 570/610 nm with a microplate reader.
Plate Format: Compatible with 96- and 384-well microplates.

Xanthine dose response was measured with Amplite® Colorimetric Xanthine Assay Kit in a 96-well clear bottom plate using a SpectraMax reader (Molecular Devices).

Catalog	Size	Price	Quantity
13842	200 Tests	Price

Overview

Xanthine is a purine base found in most human body tissues and fluids. A number of stimulants are derived from xanthine, including caffeine, aminophylline, IBMX, paraxanthine, pentoxifylline, theobromine, and theophylline, which can stimulate heart rate, force of contraction, cardiac arrhythmias at high concentrations. Therefore, detection of Xanthine alteration in biological samples is important for disease diagnosis and therapy monitoring. Amplite® Colorimetric Xanthine Assay Kit provides a quick and ultrasensitive method for the measurement of xanthine. It can be performed in a convenient 96-well or 384-well microtiter-plate format. Xanthine is oxidized to uric acid in the presence of xanthine oxidase to release hydrogen peroxide, which can be specifically measured with Amplite® Red by an absorbance microplate reader at 576 nm. With Amplite® Colorimetric Xanthine Assay Kit, as low as 1.2 µM xanthine was detected in a 100 µL reaction volume.

References

View all 60 references: Citation Explorer

Concentration-dependent effect of sodium hypochlorite on stem cells of apical papilla survival and differentiation

Authors:

Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, Diogenes A.

Journal:

J Endod (2014): 51

Effect of hypochlorite oxidation on cholinesterase-inhibition assay of acetonitrile extracts from fruits and vegetables for monitoring traces of organophosphate pesticides

Authors:

Kitamura K, Maruyama K, Hamano S, Kishi T, Kawakami T, Takahashi Y, Onodera S.

Journal:

J Toxicol Sci (2014): 71

Green synthesis of carbon dots with down- and up-conversion fluorescent properties for sensitive detection of hypochlorite with a dual-readout assay

Authors:

Yin B, Deng J, Peng X, Long Q, Zhao J, Lu Q, Chen Q, Li H, Tang H, Zhang Y, Yao S.

Journal:

Analyst (2013): 6551

Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite

Authors:

Setlow B, Yu J, Li YQ, Setlow P.

Journal:

Lett Appl Microbiol (2013): 259

A simple yet effective chromogenic reagent for the rapid estimation of bromate and hypochlorite in drinking water

Authors:

Zhang J, Yang X.

Journal:

Analyst (2013): 434

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570/610 nm
Recommended plate	Clear bottom

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Page updated on October 8, 2026

Component A: Amplite® Red Substrate (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase (lyophilized)	1 vial
Component D: Xanthine Standard	1 vial (100 µL, 20 mM)
Component E: Xanthine Oxidase (lyophilized)	1 vial
Component F: DMSO	1 vial (100 µL)