Amplite® Colorimetric Xanthine Oxidase Assay Kit

Amplite® Colorimetric Xanthine Oxidase Assay Kit is a colorimetric assay kit for quantifying xanthine oxidase activity in solution.

Detection Principle: Detects hydrogen peroxide generated during xanthine oxidation.
Sensitivity: Detects xanthine oxidase down to 0.3 mU/mL in a 100 µL reaction volume.
Reaction Chemistry: Uses xanthine substrate and Amplite® Red.
Instrument Compatibility: Absorbance detection at ~570 nm with a microplate reader.
Workflow: Compatible with 96- and 384-well plates without a separation step.

Xanthine oxidase dose response was measured with Amplite® Colorimetric Xanthine Oxidase Assay Kit in a white or black wall/clear bottom 96-well microplate using a SpectraMax microplate reader (Molecular Devices). As low as 0.12 mU/mL xanthine oxidase was detected with 30 minutes incubation time (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
11307	200 Tests	Price

Overview

Xanthine oxidase (XO)is an enzyme that catalyzes the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. It plays an important role in the catabolism of purines. Xanthine oxidase is normally found in liver and jejunum. During severe liver damage, xanthine oxidase is released into blood, so a blood assay for XO is a way to determine if liver damage has happened. Xanthinuria is a rare genetic disorder where the lack of xanthine oxidase leads to high concentration of xanthine in blood and can cause health problems such as renal failure. The Amplite® Colorimetric Xanthine Oxidase Assay Kit provides a quick and ultrasensitive method for the measurement of xanthine oxidase activities. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. In the assay, xanthine oxidase catalyzes the oxidation of purine bases, hypoxanthine or xanthine to uric acid and superoxide , which spontaneously degrades to hydrogen peroxide (H2O2). The kit uses our Amplite® Red substrate which enables a dual recordable mode. The color signal can be easily read at ~570 nm with an absorbance microplate reader. With the Amplite® Colorimetric Xanthine Oxidase Assay Kit, we have detected as little as 0.3 mU/mL xanthine oxidase in a 100 µL reaction volume.

Citations

View all 5 citations: Citation Explorer

Evaluation of anti-aging and antioxidant properties of a new rose variety, Ever-rose

Authors:

Han, Se Jik and Belousova, Polina and Kwon, Sangwoo and Jang, Jihui and Lee, Jun Bae and Kim, Hyunjae and You, Gayeon and Song, Jihyeon and Mok, Hyejung and Ha, Ho Su and others,

Journal:

Chemical and Biological Technologies in Agriculture (2024): 1--17

Protective effects of Cyclocarya paliurus on hyperuricemia and urate-induced inflammation

Authors:

Zhu, Li-Hua and Xu, Ying-Yin and Zhu, Li-ping and Zheng, Xian and Jiang, Cui-Hua and Liu, Jian-Jing and Zhang, Jian and Yin, Zhi-Qi

Journal:

Journal of Functional Foods (2022): 105130

Studies on Status of Oxidative Stress related Molecules and Enzymes in Obese with and without Diabetes in the Northern region of India

Authors:

Singh, Sukhpal and Mahajan, Amita and Kaur, Jaspreet

Journal:

Research Journal of Pharmacy and Technology (2020): 801--809

Xanthine oxidoreductase regulates macrophage IL1$\beta$ secretion upon NLRP3 inflammasome activation

Authors:

Ives, Annette and Nomura, Johji and Martinon, Fabio and Roger, Thierry and LeRoy, Didier and Miner, Jeffrey N and Simon, Gregoire and Busso, Nathalie and So, Alexander

Journal:

Nature communications (2015): 1--11

Xanthine oxidoreductase regulates macrophage IL1β secretion upon NLRP3 inflammasome activation

Authors:

Ives, Annette and Nomura, Johji and Martinon, Fabio and Roger, Thierry and LeRoy, Didier and Miner, Jeffrey N and Simon, Gregoire and Busso, Nathalie and So, Alex and er, undefined

Journal:

Nature communications (2015)

References

View all 52 references: Citation Explorer

Phenotyping of N-acetyltransferase type 2 and xanthine oxidase with caffeine: when should urine samples be collected

Authors:

Jetter A, Kinzig M, Rodamer M, Tomalik-Scharte D, Sorgel F, Fuhr U.

Journal:

Eur J Clin Pharmacol (2009): 411

Measurement of xanthine oxidase inhibition activity of phenolics and flavonoids with a modified cupric reducing antioxidant capacity (CUPRAC) method

Authors:

Ozyurek M, Bektasoglu B, Guclu K, Apak R.

Journal:

Anal Chim Acta (2009): 42

In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb

Authors:

Umamaheswari M, Asokkumar K, Sivashanmugam AT, Remyaraju A, Subhadradevi V, Ravi TK.

Journal:

J Ethnopharmacol (2009): 646

Increased xanthine oxidase in the skin of preeclamptic women

Authors:

Bainbridge SA, Deng JS, Roberts JM.

Journal:

Reprod Sci (2009): 468

Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice

Authors:

Chatterjee S, Ehrenshaft M, Bhattacharjee S, Deterding LJ, Bonini MG, Corbett J, Kadiiska MB, Tomer KB, Mason RP.

Journal:

Free Radic Biol Med (2009): 454

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570/610 nm
Recommended plate	Clear bottom

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Fax
Email	sales@aatbio.com
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Page updated on October 8, 2026

Component A: Amplite® Red (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (20 mL)
Component C: Horseradish Peroxidase	1 vial (lyophilized)
Component D: Xanthine	1 vial (100 µL, 100X)
Component E: Xanthine Oxidase Standard	1 vial (200 mU, lyophilized)
Component F: DMSO	1 vial (200 µL)