Name: Amplite® Colorimetric Zinc Ion Quantitation Kit
Brand: AAT Bioquest
SKU: 19001
Price: 237 USD
Availability: InStock

Amplite® Colorimetric Zinc Ion Quantitation Kit

Amplite® Colorimetric Zinc Ion Quantitation Kit is a colorimetric assay kit for quantifying zinc ions in biological samples such as serum, plasma, and urine.

Detection Principle: Uses Zn-620™ for zinc-dependent colorimetric detection.
Sensitivity: Detects zinc ions as low as 0.1 uM.
Specificity: Shows minimal response to Ca²⁺ and Mg²⁺.
Signal Advantage: Produces a >100-fold increase in absorption with Zn²⁺.
Instrument Compatibility: Absorbance detection at 610/470 nm with a microplate reader.

Zinc Chloride dose response was measured on a 96-well clear bottom plate with the Amplite® Colorimetric Zinc Ion Quantitation Kit.

Protocol

SDS

Catalog	Size	Price	Quantity
19001	200 Tests	Price

Overview

Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring are highly desirable in research and drug discovery. AAT Bioquest's Amplite® Colorimetric Zinc Quantitation Kit provides a simple method for detecting zinc concentration in biological samples using our proprietary Zn-620™, in which Zinc binds to the probe with the enhanced absorption around 620 nm. The Zinc probe exhibits a large increase in 620 nm absorption in response to Zn2+ (>100 folds). Our kit formulation has enhanced Zn2+-specificity with little responses to other metals, e.g., Ca2+ and Mg2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 1 µM. Our Amplite® Fluorimetric Zinc Ion Quantitation Kit (#19000) is even more sensitive, and can be used for detecting as low as 0.1 uM zinc ion.

Citations

View all 4 citations: Citation Explorer

Growth Inhibition of Gram-Positive and Gram-Negative Bacteria by Zinc Oxide Hedgehog Particles

Authors:

Rutherford, David and J{\'\i}ra, Jaroslav and Kol{\'a}{\v{r}}ov{\'a}, Kate{\v{r}}ina and Matol{\'\i}nov{\'a}, Iva and Mi{\v{c}}ov{\'a}, J{\'u}lia and Reme{\v{s}}, Zdenek and Rezek, Bohuslav

Journal:

International Journal of Nanomedicine (2021): 3541

Kinetic Effects of Heat Stress on Olfaction: A Thermodynamic Evaluation of Electrical Responses to Odorants in Olfactory Epithelia

Authors:

Hagerty, Samantha

The role of zinc nanoparticles in the initial events of olfaction, their characterization, preservation, and microenvironmental influence.

Authors:

Singletary, Melissa

After oxidation, zinc nanoparticles lose their ability to enhance responses to odorants

Authors:

Hagerty, Samantha and Daniels, Yasmine and Singletary, Melissa and Pustovyy, Oleg and Globa, Ludmila and MacCrehan, William A and Muramoto, Shin and Stan, Gheorghe and Lau, June W and Morrison, Edward E and others, undefined

Journal:

BioMetals (2016): 1--14

References

View all 46 references: Citation Explorer

Reaction of metal-binding ligands with the zinc proteome: zinc sensors and N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine

Authors:

Meeusen JW, Nowakowski A, Petering DH.

Journal:

Inorg Chem (2012): 3625

EAAC1 gene deletion alters zinc homeostasis and enhances cortical neuronal injury after transient cerebral ischemia in mice

Authors:

Jang BG, Won SJ, Kim JH, Choi BY, Lee MW, Sohn M, Song HK, Suh SW.

Journal:

J Trace Elem Med Biol (2012): 85

TSQ (6-methoxy-8-p-toluenesulfonamido-quinoline), a common fluorescent sensor for cellular zinc, images zinc proteins

Authors:

Meeusen JW, Tomasiewicz H, Nowakowski A, Petering DH.

Journal:

Inorg Chem (2011): 7563

Dependence of the histofluorescently reactive zinc pool on zinc transporter-3 in the normal brain

Authors:

Lee JY, Kim JS, Byun HR, Palmiter RD, Koh JY.

Journal:

Brain Res (2011): 12

Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution

Authors:

Nowakowski AB, Petering DH.

Journal:

Inorg Chem (2011): 10124

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	610/470 nm
Recommended plate	Clear bottom

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Fax
Email	sales@aatbio.com
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Page updated on October 8, 2026

Component A: Zn-620™	1 vial (50 µL)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: ZnCl2 Standard	1 vial (100 mM, 100 µL)