Amplite® Ethanol Quantitation Kit

Amplite® Ethanol Quantitation Kit enables rapid and sensitive measurement of ethanol using a dual-mode readout.

Dual detection formats: Compatible with both fluorescence and absorbance plate readers for flexible assay design
Fast and simple workflow: Mix-and-read format allows completion within 30 minutes
High sensitivity and robustness: Detects low levels of ethanol with reliable performance in various sample types
Comparable alternative: Offers a reliable, non-radioactive solution similar to Abcam's ethanol assay kits

Ethanol dose response was measured with Amplite® Ethanol Quantitation Kit on a solid black 96-well plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
40001	200 Tests	Price

Overview

Amplite® Ethanol Quantitation Kit is a fast, non-radioactive assay designed for the sensitive detection of ethanol in biological and industrial samples. Based on the enzymatic oxidation of ethanol by alcohol oxidase, which is then detected using Amplite® Red, a proprietary fluorogenic and chromogenic substrate.

The kit supports both fluorimetric and colorimetric detection modes, allowing flexible integration into diverse assay platforms. With a simple mix-and-read protocol, the assay is completed in under 30 minutes and is easily scalable for high-throughput applications. It is ideal for life science research, clinical evaluations, and ethanol quantitation in food and beverage testing.

Citations

View all 9 citations: Citation Explorer

SeeThrough: a rationally designed skull clearing technique for in vivo brain imaging

Authors:

Liu, Xinyi and Uchigashima, Motokazu and Oomoto, Ikumi and Saito, Yoshihito and Uchida, Hitoshi and Oginezawa, Shinya and Masuda, Keiko and Satoh, Daisuke and Abe, Manabu and Sakimura, Kenji and others,

Journal:

Nature Communications (2025): 7584

Light-activatable minimally invasive ethyl cellulose ethanol ablation: Biodistribution and potential applications

Authors:

Yang, Jeffrey and Ma, Chen-Hua and Quinlan, John A and McNaughton, Kathryn and Lee, Taya and Shin, Peter and Hauser, Tessa and Kaluzienski, Michele L and Vig, Shruti and Quang, Tri T and others,

Journal:

Bioengineering \& Translational Medicine (2024): e10696

TAT-surface modified acyclovir-loaded albumin nanoparticles as a novel ocular drug delivery system

Authors:

Suwannoi, Panita and Chomnawang, Mullika and Tunsirikongkon, Amolnat and Phongphisutthinan, Angsuma and Müller-Goymann, Christel C and Sarisuta, Narong

Journal:

Journal of Drug Delivery Science and Technology (2019)

Novel Role of Ethanol in Delaying Postharvest Physiological Deterioration and Keeping Quality in Cassava

Authors:

Liu, Guoyin and Li, Bing and Wang, Yuqi and Wei, Bo and He, Chaozu and Liu, Debing and Shi, Haitao

Journal:

Food and Bioprocess Technology (2019): 1--10

Development of acyclovir-loaded albumin nanoparticles and improvement of acyclovir permeation across human corneal epithelial T cells

Authors:

Suwannoi, Panita and Chomnawang, Mullika and Sarisuta, Narong and Reichl, Stephan and M{\"u}ller-Goymann, Christel C

Journal:

Journal of Ocular Pharmacology and Therapeutics (2017): 743--752

References

View all 3 references: Citation Explorer

Prevalence of ethanol consumption may be higher in women than men in a university health service population as determined by a biochemical marker: whole blood-associated acetaldehyde above the 99th percentile for teetotalers

Authors:

Peterson KP, Bowers C, Peterson CM.

Journal:

J Addict Dis (1998): 13

Quantifying ethanol by high performance liquid chromatography with precolumn enzymatic conversion and derivatization with fluorimetric detection

Authors:

Chen HM, Peterson CM.

Journal:

Alcohol (1994): 577

Enzymatic determination of ethanol in saliva by flow injection analysis

Authors:

Linares P, Ruz J, De Castro MD, Valcarcel M.

Journal:

J Pharm Biomed Anal (1987): 701

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	576 ± 5 nm
Recommended plate	Clear bottom

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 27, 2025

Component A: Amplite® Ethanol Reagent (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (10 mL)
Component C: Ethanol Enzyme Mix (lyophilized)	1 vial
Component D: DMSO	1 vial (200 µL)
Component E: Ethanol Standard	(100%, 0.5 mL)