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Amplite® Fluorimetric Acetylcholinesterase Assay Kit
Green Fluorescence
Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity- each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite® Fluorimetric Acetylcholinesterase Assay Kit provides the most sensitive method for the detecting AChE activity. The kit uses our outstanding Thiolite Green™ to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The fluorescence intensity of Thiolite Green™ is proportional to the formation of thiolcholine, thus the AChE activity.
<p>The relationships among Vt by Logan plot analysis, SUV at 30 min, radioactivity count by well counter, and AChE activity by fluorometric assay. *The AChE activities and ACh levels were quantified using the AmpliteTM Fluorimetric Acetylcholinesterase Assay Kit (AAT Bioquest, Inc). Source: Table from <strong>Distribution of Intravenously Administered Acetylcholinesterase Inhibitor and Acetylcholinesterase Activity in the Adrenal Gland: 11C-Donepezil PET Study in the Normal Rat</strong> by Tadashi Watabe et al., <em>PLOS</em>, Sep. 2014.&nbsp;</p>
<p>The relationships among Vt by Logan plot analysis, SUV at 30 min, radioactivity count by well counter, and AChE activity by fluorometric assay. *The AChE activities and ACh levels were quantified using the AmpliteTM Fluorimetric Acetylcholinesterase Assay Kit (AAT Bioquest, Inc). Source: Table from <strong>Distribution of Intravenously Administered Acetylcholinesterase Inhibitor and Acetylcholinesterase Activity in the Adrenal Gland: 11C-Donepezil PET Study in the Normal Rat</strong> by Tadashi Watabe et al., <em>PLOS</em>, Sep. 2014.&nbsp;</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11401200 Tests
Price
 
Spectral properties
Excitation (nm)505
Emission (nm)524
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on September 21, 2025