Name: Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 11401
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Acetylcholinesterase Assay Kit

Green Fluorescence

Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence* is a fluorimetric assay kit for quantification of acetylcholinesterase activity in a fluorescence microplate format.

Detection Principle: Quantifies thiocholine produced from acetylthiocholine hydrolysis.
Sensitivity: Detects AChE activity down to 0.1 mU/mL in a 100 µL assay volume.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm.
Workflow: Provides a one-step fluorimetric assay format.

<p>The relationships among Vt by Logan plot analysis, SUV at 30 min, radioactivity count by well counter, and AChE activity by fluorometric assay. *The AChE activities and ACh levels were quantified using the AmpliteTM Fluorimetric Acetylcholinesterase Assay Kit (AAT Bioquest, Inc). Source: Table from <strong>Distribution of Intravenously Administered Acetylcholinesterase Inhibitor and Acetylcholinesterase Activity in the Adrenal Gland: 11C-Donepezil PET Study in the Normal Rat</strong> by Tadashi Watabe et al., <em>PLOS</em>, Sep. 2014. </p>

Protocol

SDS

Catalog	Size	Price	Quantity
11401	200 Tests	Price

Overview

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity- each molecule of AChE degrades about 5000 molecules of acetylcholine per second. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. This Amplite® Fluorimetric Acetylcholinesterase Assay Kit provides the most sensitive method for the detecting AChE activity. The kit uses our outstanding Thiolite Green™ to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The fluorescence intensity of Thiolite Green™ is proportional to the formation of thiolcholine, thus the AChE activity.

Citations

View all 40 citations: Citation Explorer

ME as a Multi-Target-Directed Ligand for Alzheimer’s Disease: A Promising Lead for Drug Design

Authors:

Aiswarya, N and Remya, Chandran and Sajitha, K and Rajan, Rakhi and Vasudevan, DM and Dileep, KV

Journal:

ASPET Discovery (2025): 100019

The complex interplay between ligand strain, coulombic interactions, and binding site dynamics of two congeneric AChE inhibitors

Authors:

Babu, Aravinda and Remya, Chandran and Sajith, AM and Dileep, KV and Kenchaiah, Sunil and Khade, Ajit and Zyryanov, Grigory V and Joy, Muthipeedika Nibin

Journal:

Journal of Molecular Structure (2025): 141479

Aluminum chloride and D-galactose induced a zebrafish model of Alzheimer's disease with cognitive deficits and aging

Authors:

Luo, Li and Yan, Tao and Yang, Le and Zhao, Minggao

Journal:

Computational and Structural Biotechnology Journal (2024)

Identification of Potent and Selective Acetylcholinesterase/Butyrylcholinesterase Inhibitors by Virtual Screening

Authors:

Xu, Tuan and Li, Shuaizhang and Li, Andrew J and Zhao, Jinghua and Sakamuru, Srilatha and Huang, Wenwei and Xia, Menghang and Huang, Ruili

Journal:

Journal of Chemical Information and Modeling (2023)

Imaging mass spectrometry to visualise increased acetylcholine in lungs of asthma model mice

Authors:

Matsuda, Takeshi and Suzuki, Yuzo and Fujisawa, Tomoyuki and Suga, Yasunori and Saito, Nobuyuki and Suda, Takafumi and Yao, Ikuko

Journal:

Analytical and Bioanalytical Chemistry (2020): 1--15

Spectral properties

Excitation (nm)	505
Emission (nm)	524

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Thiolite™ Green	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Acetylthiocholine	1 vial
Component D: Acetylcholinesterase Standard	1 vial (5 units)
Component E: DMSO	1 vial (100 µL)