Name: Amplite® Fluorimetric Aldehyde Quantitation Kit
Brand: AAT Bioquest
SKU: 10052
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Aldehyde Quantitation Kit

Amplite® Fluorimetric Aldehyde Quantitation Kit is a fluorimetric assay kit for quantification of aldehydes in a microplate format.

Detection Principle: Forms a fluorescent product upon reaction with aldehydes.
Sensitivity: Detects aldehydes down to 1 µM.
Workflow/Instrument Compatibility: Mix-and-read assay for 96- or 384-well plates.
Applications: Supports aldehyde quantification in biological, chemical, food, and environmental research.

Aldehyde dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Aldehyde Quantitation Kit using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
10052	200 Tests	Price

Overview

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years. Rapid and accurate measurement of aldehydes is an important task for biological research, chemical research, food industry and environmental pollution surveillance. There are a few reagents or assay kits available for quantifying the number of aldehydes. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. Both our Amplite® Colorimetric Aldehyde Quantitation Kit (10051) and Amplite® Fluorimetric Aldehyde Quantitation kit (10052) are used for quantifying aldehydes at higher pH. Kit 10052 uses a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with an aldehyde. Kit 10052 is much more sensitive than Kit 10051. This fluorimetric kit provides a sensitive mix-and-read method to detect as little as 0.1 nanomole of aldehyde in a 100 µL assay volume (1 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader.

Citations

View all 8 citations: Citation Explorer

Excessive linoleic acid induces muscle oxidative stress through 5-lipoxygenase-dependent peroxidation

Authors:

Cao, Xiufei and Guo, Huixing and Dai, Yongjun and Jiang, Guangzhen and Liu, Wenbin and Li, Xiangfei and Zhang, Dingdong and Huang, Yangyang and Wang, Xi and Hua, Haokun and others,

Journal:

Redox Biology (2024): 103096

Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage

Authors:

Zhou, Junmei and Sun, Chunbao and Yang, Lu and Wang, Jinhui and Jn-Simon, Natacha and Zhou, Chen and Bryant, Andrew and Cao, Qi and Li, Chenglong and Petersen, Bryon and others,

Journal:

The FASEB Journal (2022): e22224

Inhibition of ALDH2 by O-GlcNAcylation contributes to the hyperglycemic exacerbation of myocardial ischemia/reperfusion injury

Authors:

Liu, Baoshan and Wang, Jiali and Li, Minghua and Yuan, Qiuhuan and Xue, Mengyang and Xu, Feng and Chen, Yuguo

Journal:

Oncotarget (2017): 19413

Hepatic Deficiency of Augmenter of Liver Regeneration Exacerbates Alcohol-Induced Liver Injury and Promotes Fibrosis in Mice

Authors:

Kumar, Sudhir and Wang, Jiang and Rani, Richa and G, undefined and hi, Ch and rashekhar R, undefined

Journal:

PloS one (2016): e0147864

Integrated self-assembling drug delivery system possessing dual responsive and active targeting for orthotopic ovarian cancer theranostics

Authors:

Lin, Chun-Jui and Kuan, Chen-Hsiang and Wang, Li-Wen and Wu, Hsi-Chin and Chen, Yunching and Chang, Chien-Wen and Huang, Rih-Yang and Wang, Tzu-Wei

Journal:

Biomaterials (2016): 12--26

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	365 nm
Emission	435 nm
Cutoff	420 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: AldeLight™ Blue	1 vial
Component B: Assay Buffer	1 bottle (30 mL)
Component C: Reaction Buffer	1 vial (6 mL)
Component D: Aldehyde Standard	1 vial
Component E: DMSO	1 vial (100 µL)