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Amplite® Fluorimetric Aldehyde Quantitation Kit
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years. Rapid and accurate measurement of aldehydes is an important task for biological research, chemical research, food industry and environmental pollution surveillance. There are a few reagents or assay kits available for quantifying the number of aldehydes. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS. Both our Amplite® Colorimetric Aldehyde Quantitation Kit (10051) and Amplite® Fluorimetric Aldehyde Quantitation kit (10052) are used for quantifying aldehydes at higher pH. Kit 10052 uses a proprietary fluorogenic dye that generates a strongly fluorescent product upon reacting with an aldehyde. Kit 10052 is much more sensitive than Kit 10051. This fluorimetric kit provides a sensitive mix-and-read method to detect as little as 0.1 nanomole of aldehyde in a 100 µL assay volume (1 µM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Its signal can be easily read by a fluorescence microplate reader.
Aldehyde dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Aldehyde Quantitation Kit using a Gemini fluorescence microplate reader (Molecular Devices).
Aldehyde dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Aldehyde Quantitation Kit using a Gemini fluorescence microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
10052200 Tests
Price
 
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation365 nm
Emission435 nm
Cutoff420 nm
Recommended plateSolid black
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Page updated on September 25, 2025