Name: Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 11952
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit

Blue Fluorescence

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence* is a fluorimetric assay kit for quantification of alkaline phosphatase activity in solutions, cell extracts, and on solid surfaces.

Detection Principle: Uses MUP as a fluorogenic phosphatase substrate.
Sensitivity: Detects alkaline phosphatase down to 0.01 mU/well.
Workflow: Optimized mix-and-read assay compatible with HTS liquid handling.
Instrument Compatibility: Fluorescence detection at Ex/Em 360/450 nm with a microplate reader.

Alkaline phosphatase dose response was measured with Amplite® Fluorimetric Alkaline Phosphatase Assay Kit in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices). As low as 0.01 mU/well of alkaline phosphatase can be detected with 30 minutes incubation (n=3). RFU read at Ex/Em = 360/450 nm (Cutoff = 435 nm).

Protocol

SDS

Catalog	Size	Price	Quantity
11952	500 Tests	Price

Overview

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite® Alkaline Phosphatase Assay Kit uses MUP, a fluorogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts as well as on solid surfaces (such as PVDF membranes). The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

Citations

View all 9 citations: Citation Explorer

The impact of various scaffold components on vascularized bone constructs

Authors:

Eweida, Ahmad and Schulte, Matthias and Frisch, Oliver and Kneser, Ulrich and Harhaus, Leila

Journal:

Journal of Cranio-Maxillofacial Surgery (2017)

A Mineralized High Strength and Tough Hydrogel for Skull Bone Regeneration

Authors:

Xu, Bing and Zheng, Pengbin and Gao, Fei and Wang, Wei and Zhang, Hongtao and Zhang, Xuran and Feng, Xuequan and Liu, Wenguang

Journal:

Advanced Functional Materials (2016)

DRG axon elongation and growth cone collapse rate induced by Sema3A are differently dependent on NGF concentration

Authors:

Kaselis, Andrius and Treinys, Rimantas and Vosyliute, Ruta and Satkauskas, Saulius

Journal:

Cellular and molecular neurobiology (2014): 289--296

Acute oral toxicity and kinetic behaviors of inorganic layered nanoparticles

Authors:

Yu, Jin and Chung, Hea-Eun and Choi, Soo-Jin

Journal:

Journal of Nanomaterials (2013): 12

Effect of Some Antihypertensive Drugs on Alkaline Phosphatase and DNA of Mice

Authors:

El-Khawaga, OY and El-Waseef, A and Ellazec, YO and El-Naggar, MM and Alla, Abd M

Journal:

International Journal of Genomics and Proteomics (2013): 60

References

View all 109 references: Citation Explorer

8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline phosphatase

Authors:

Zhu X, Jiang C.

Journal:

Clin Chim Acta. (2006)

Effects of hydrogen peroxide (H(2)O(2)) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines

Authors:

Lee DH, Lim BS, Lee YK, Yang HC.

Journal:

Cell Biol Toxicol (2006): 39

The effect of alkaline phosphatase inhibitors on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue

Authors:

Ali AT, Penny CB, Paiker JE, Psaras G, Ikram F, Crowther NJ.

Journal:

Ann Clin Biochem (2006): 207

Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1

Authors:

Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ.

Journal:

Clin Chim Acta (2005): 101

Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study

Authors:

Rieu JP, Ronzon F, Place C, Dekkiche F, Cross B, Roux B.

Journal:

Acta Biochim Pol (2004): 189

Spectral properties

Excitation (nm)	360
Emission (nm)	448

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	360 nm
Emission	450 nm
Cutoff	435 nm
Recommended plate	Solid black

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Email	sales@aatbio.com
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Page updated on October 6, 2026

Component A: MUP Plus™ (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Alkaline Phosphatase Standard	1 vial (lyophilized powder, 10 units)