Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit

Green Fluorescence

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence* is a fluorimetric assay kit for quantification of alkaline phosphatase activity in solutions, cell extracts, and on solid surfaces.

Detection Principle: Uses FDP as a fluorogenic phosphatase substrate.
Workflow: Optimized mix-and-read assay compatible with HTS liquid handling.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm with a microplate reader.
Applications: Suitable for ALP measurements in immunoassays and related biological assays.

Alkaline phosphatase dose response was measured with the Amplite® Fluorimetric Alkaline Phosphatase Assay Kit in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
11953	500 Tests	Price

Overview

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite® Alkaline Phosphatase Assay Kit uses FDP, a sensitive fluorogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts as well as on solid surfaces (such as PVDF membranes). The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.

Citations

View all 12 citations: Citation Explorer

Bacteriophage-Based Rapid Bacteria Detection

Authors:

Wisuthiphaet, Nicharee

Development and validation of bioengineered intestinal tubules for translational research aimed at safety and efficacy testing of drugs and nutrients

Authors:

Jochems, Paulus GM and van Bergenhenegouwen, Jeroen and van Genderen, Anne Metje and Eis, Sophie T and Versprille, Livia JF Wilod and Wichers, Harry J and Jeurink, Prescilla V and Garssen, Johan and Masereeuw, Rosalinde

Journal:

Toxicology in Vitro (2019)

Rapid detection of Escherichia coli in beverages using genetically engineered bacteriophage T7

Authors:

Wisuthiphaet, Nicharee and Yang, Xu and Young, Glenn M and Nitin, Nitin

Journal:

AMB Express (2019): 55

The impact of various scaffold components on vascularized bone constructs

Authors:

Eweida, Ahmad and Schulte, Matthias and Frisch, Oliver and Kneser, Ulrich and Harhaus, Leila

Journal:

Journal of Cranio-Maxillofacial Surgery (2017)

A Mineralized High Strength and Tough Hydrogel for Skull Bone Regeneration

Authors:

Xu, Bing and Zheng, Pengbin and Gao, Fei and Wang, Wei and Zhang, Hongtao and Zhang, Xuran and Feng, Xuequan and Liu, Wenguang

Journal:

Advanced Functional Materials (2016)

References

View all 109 references: Citation Explorer

The effect of alkaline phosphatase inhibitors on intracellular lipid accumulation in preadipocytes isolated from human mammary tissue

Authors:

Ali AT, Penny CB, Paiker JE, Psaras G, Ikram F, Crowther NJ.

Journal:

Ann Clin Biochem (2006): 207

Effects of hydrogen peroxide (H(2)O(2)) on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines

Authors:

Lee DH, Lim BS, Lee YK, Yang HC.

Journal:

Cell Biol Toxicol (2006): 39

8-Quinolyl phosphate as a substrate for the fluorimetric determination of alkaline phosphatase

Authors:

Zhu X, Jiang C.

Journal:

Clin Chim Acta. (2006)

Alkaline phosphatase is involved in the control of adipogenesis in the murine preadipocyte cell line, 3T3-L1

Authors:

Ali AT, Penny CB, Paiker JE, van Niekerk C, Smit A, Ferris WF, Crowther NJ.

Journal:

Clin Chim Acta (2005): 101

Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study

Authors:

Rieu JP, Ronzon F, Place C, Dekkiche F, Cross B, Roux B.

Journal:

Acta Biochim Pol (2004): 189

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Fax
Email	sales@aatbio.com
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Page updated on October 2, 2026

Component A: FDP (light sensitive)	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Alkaline Phosphatase Standard	1 vial (lyophilized powder, 10 units)
Component D: DMSO	1 vial (200 µL)
Component E: Stop Solution	1 bottle (25 mL)