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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit
Green Fluorescence
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite® Alkaline Phosphatase Assay Kit uses FDP, a sensitive fluorogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts as well as on solid surfaces (such as PVDF membranes). The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
Alkaline phosphatase dose response was measured with the Amplite® Fluorimetric Alkaline Phosphatase Assay Kit in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).
Alkaline phosphatase dose response was measured with the Amplite® Fluorimetric Alkaline Phosphatase Assay Kit in a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
11953500 Tests
Price
 
Spectral properties

Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
0.95
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings

Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on October 9, 2025