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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit
Near Infrared Fluorescence
Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications. It is widely used in various biological assays (in particular, immunoassays) and ELISA-based diagnostics. This Amplite® Alkaline Phosphatase Assay Kit uses a proprietary flurogenic phosphatase substrate, to quantify alkaline phosphatase activity in solutions, in cell extracts as well as on solid surfaces (such as PVDF membranes). This proprietary flurogenic phosphatase substrate generates a fluorescent product that has strongly red fluorescence upon interaction with phosphatase. The kit provides all the essential components with our optimized 'mix and read' assay protocol that is compatible with HTS liquid handling instruments.
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 11954 | 500 Tests | Price |
Spectral properties
| Correction factor (280 nm) | 0.366 |
| Excitation (nm) | 592 |
| Emission (nm) | 609 |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| UNSPSC | 12171501 |
Instrument settings
| Fluorescence microplate reader | |
| Excitation | 620 nm |
| Emission | 660 nm |
| Cutoff | 630 nm |
| Recommended plate | Solid black |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 13, 2025