Name: Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Brand: AAT Bioquest
SKU: 10087
Price: 475 USD
Availability: InStock

Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit is a fluorimetric assay kit for quantification of α-ketoglutarate in biological samples.

Detection Principle: Detects hydrogen peroxide generated by an enzyme-coupled reaction.
Sensitivity: Detects α-ketoglutarate down to 1 µM.
Detection Chemistry: Uses Amplite® Red for fluorescent detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.

Alpha-ketoglutarate dose response was measured with the Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit in a 96-well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices) at Ex/Em = 540/590 nm, cutoff = 570 nm. As low as 1 µM of α-ketoglutarate can be detected with 30 minutes incubation.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
10087	200 Tests	Price

Overview

Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism. As a precursor of glutamate and glutamine, α-ketoglutarate is a central metabolic fuel for cells of the gastrointestinal tract as well. It can decrease protein catabolism and increase protein synthesis to enhance bone tissue formation in the skeletal muscles and can be used in clinical applications. Alpha-ketoglutarate is used for kidney disease; intestinal and stomach disorders, including bacterial infections; liver problems; cataracts; and recurring yeast infections. It is also used for improving the way kidney patients receiving hemodialysis treatments process protein. Amplite®™ Fluorimetric α-Ketoglutarate Quantitation Kit offers a sensitive fluorimetric assay for quantifying α-ketoglutarate in biological samples. It utilizes an enzyme coupled reaction that releases hydrogen peroxide, which can be detected with Amplite®™ Red at Ex/Em ~540/590 nm (red fluorescence).

References

View all 40 references: Citation Explorer

Loss of Nardilysin, a Mitochondrial Co-chaperone for alpha-Ketoglutarate Dehydrogenase, Promotes mTORC1 Activation and Neurodegeneration

Authors:

Yoon WH, S and oval H, Nagarkar-Jaiswal S, Jaiswal M, Yamamoto S, Haelterman NA, Putluri N, Putluri V, Sreekumar A, Tos T, Aksoy A, Donti T, Graham BH, Ohno M, Nishi E, Hunter J, Muzny DM, Carmichael J, Shen J, Arboleda VA, Nelson SF, Wangler MF, Karaca E, Lupski JR, Bellen HJ.

Journal:

Neuron (2017): 115

Dietary alpha-ketoglutarate promotes higher protein and lower triacylglyceride levels and induces oxidative stress in larvae and young adults but not in middle-aged Drosophila melanogaster

Authors:

Bayliak MM, Lylyk MP, Shmihel HV, Sorochynska OM, Semchyshyn OI, Storey JM, Storey KB, Lushchak VI.

Journal:

Comp Biochem Physiol A Mol Integr Physiol (2017): 28

The facial triad in the alpha-ketoglutarate dependent oxygenase FIH: A role for sterics in linking substrate binding to O2 activation

Authors:

Hangasky JA, Taabazuing CY, Martin CB, Eron SJ, Knapp MJ.

Journal:

J Inorg Biochem (2017): 26

Identification of a Chemoreceptor in Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward alpha-Ketoglutarate

Authors:

Martin-Mora D, Ortega A, Reyes-Darias JA, Garcia V, Lopez-Farfan D, Matilla MA, Krell T.

Journal:

Front Microbiol (2016): 1937

Dietary Supplementation with alpha-Ketoglutarate Activates mTOR Signaling and Enhances Energy Status in Skeletal Muscle of Lipopolysaccharide-Challenged Piglets

Authors:

Wang L, Yi D, Hou Y, Ding B, Li K, Li B, Zhu H, Liu Y, Wu G.

Journal:

J Nutr (2016): 1514

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 2, 2026

Component A: Amplite® Red	1 vial
Component B1: Enzyme Mix 1	2 bottles (lyophilized powder)
Component B2: Enzyme Mix 2	2 vials (lyophilized powder)
Component C: Assay Buffer	1 bottle (10 mL)
Component D: α-Ketoglutarate Standard	1 vial (10 mM, 100 µL)
Component E: DMSO	1 vial (100 µL)