Name: Amplite® Fluorimetric Ascorbic Acid Assay Kit
Brand: AAT Bioquest
SKU: 13835
Price: 415 USD
Availability: InStock

Amplite® Fluorimetric Ascorbic Acid Assay Kit

Amplite® Fluorimetric Ascorbic Acid Assay Kit is a fluorimetric assay kit for quantification of total ascorbic acid and the DHA-to-ascorbic acid ratio in biological samples.

Detection Principle: Oxidizes ascorbic acid to DHA for fluorescent detection.
Sensitivity: Detects total ascorbate down to 1 µM.
Detection Chemistry: Uses Ascorbrite™ Blue for DHA detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 340/430 nm with a microplate reader.

Ascorbic acid dose response was measured with Amplite® Fluorimetric Ascorbic Acid Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13835	200 Tests	Price

Overview

L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense. Ascorbate is produced from glucose in the liver of most mammalian species. For humans ascorbate has to be obtained from food to survive, and a lack of sufficient Vitamin C can result in scurvy, and may eventually lead to death. As an antioxidant ascorbate can reduce the risk of developing chronic disease such as cancer and cardiovascular disease. In food industry, ascorbic acid and its sodium, potassium, and calcium salts are commonly used as antioxidant food additives to prevent undesired color and taste. AAT Bioquest's Amplite® Fluorimetric Ascorbic Acid Assay Kit offers a sensitive fluorescent assay for quantifying total ascorbic acid and the ratio of dehydroascorbic acid (DHA) to ascorbic acid in biological samples. It utilizes an enzyme reaction that oxidize ascorbic acid to DHA, which can be detected by Ascorbrite™ Blue with a fluorescence microplate reader. The assay can detect 1uM of total ascorbate in a sample.

References

View all 47 references: Citation Explorer

Rapid monitoring of glycerol in fermentation growth media: Facilitating crude glycerol bioprocess development

Authors:

Abad S, Perez X, Planas A, Turon X.

Journal:

Talanta (2014): 210

Synthesis of substituted 1,3-diesters of glycerol using wittig chemistry

Authors:

Lowe HI, Toyang NJ, Watson CT, Bryant J.

Journal:

Nat Prod Commun (2014): 687

The glycoglycerolipid 1,2-dipalmitoyl-3-(N-palmitoyl-6'-amino-6'-deoxy-alpha-d-glucosyl)-sn-glycerol is no inhibitor of the human Myt1 kinase

Authors:

Rohe A, Gollner C, Erdmann F, Sippl W, Schmidt M.

Journal:

J Enzyme Inhib Med Chem (2014): 514

Functional characterization of Yersinia pestis aerobic glycerol metabolism

Authors:

Willias SP, Chauhan S, Motin VL.

Journal:

Microb Pathog (2014): 33

Quantitative analysis of glycerol levels in human urine by liquid chromatography-tandem mass spectrometry

Authors:

Dong Y, Ma Y, Yan K, Shen L, Wang X, Xu Y, He G, Wu Y, Lu J, Yang Z, Feng F.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2014): 30

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	340 nm
Emission	430 nm
Cutoff	420 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Ascorbrite™ Blue	1 vial
Component B: Enzyme Mix	2 bottles (lyophilized powder)
Component C: Assay Buffer	1 bottle (20 mL)
Component D: Ascorbic Acid Standard (MW=176.1)	1 vial (3.52 mg)
Component E: DMSO	1 vial (100 µL)