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Amplite® Fluorimetric Ascorbic Acid Assay Kit
L-Ascorbic Acid (also called Vitamin C) is a critical metabolite for both plant and animals in cell division, growth and defense. Ascorbate is produced from glucose in the liver of most mammalian species. For humans ascorbate has to be obtained from food to survive, and a lack of sufficient Vitamin C can result in scurvy, and may eventually lead to death. As an antioxidant ascorbate can reduce the risk of developing chronic disease such as cancer and cardiovascular disease. In food industry, ascorbic acid and its sodium, potassium, and calcium salts are commonly used as antioxidant food additives to prevent undesired color and taste. AAT Bioquest's Amplite® Fluorimetric Ascorbic Acid Assay Kit offers a sensitive fluorescent assay for quantifying total ascorbic acid and the ratio of dehydroascorbic acid (DHA) to ascorbic acid in biological samples. It utilizes an enzyme reaction that oxidize ascorbic acid to DHA, which can be detected by Ascorbrite™ Blue with a fluorescence microplate reader. The assay can detect 1uM of total ascorbate in a sample.
Ascorbic acid dose response was measured with Amplite® Fluorimetric Ascorbic Acid Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).
Ascorbic acid dose response was measured with Amplite® Fluorimetric Ascorbic Acid Assay Kit on a solid black 96-well plate using a Gemini microplate reader (Molecular Devices).
CatalogSize
Price
Quantity
13835200 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation340 nm
Emission430 nm
Cutoff420 nm
Recommended plateSolid black
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Page updated on October 12, 2025