Name: Amplite® Fluorimetric Asparaginase Activity Assay Kit
Brand: AAT Bioquest
SKU: 11799
Price: 516 USD
Availability: InStock

Amplite® Fluorimetric Asparaginase Activity Assay Kit

Amplite® Fluorimetric Asparaginase Activity Assay Kit is a fluorimetric assay kit for quantification of asparaginase activity in biological samples.

Detection Principle: Uses a coupled enzyme assay to detect aspartate production.
Signal Response: Fluorescence is proportional to aspartate generated by asparaginase.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm with a microplate reader.
Applications: Suitable for asparaginase activity measurements in biological samples.

Aspartate dose response was measured using the Amplite® Fluorimetric Asparaginase Activity Assay Kit on a 96-well clear bottom black solid microplate using a fluorescence microplate reader (Ex/Em = 540/590, Cutoff = 570 nm).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11799	100 Tests	Price

Overview

The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples. Asparaginase activity is determined by a coupled enzyme assay, which results in the formation of a fluorogenic product with Ex/Em=540/590nm. The amount of the fluorogenic product formed is proportional to the aspartate generated by asparaginase enzyme. 1 unit (U) is the amount of enzyme that catalyzes the reaction of 1 µmol of substrate per minute. Asparaginase is an essential enzyme that catalyzes the hydrolysis of the non-essential amino acid asparagine to aspartate and ammonia. It plays a crucial role in cellular functions, particularly in hematopoietic cells which rely on exogenous asparagine for protein synthesis. Asparaginase is found in plants, microorganisms, and certain animals, but does not occur naturally in humans, making it a valuable therapeutic agent in medicine and an essential tool in various industries. Asparaginase is used to treat acute lymphocytic leukemia (ALL) by starving tumor cells of needed nutrients and slowing tumor cell growth. Depletion of circulating asparagine by asparaginase induces cell cycle arrest and apoptosis in malignant cells, offering a targeted approach to cancer treatment. Beyond its medical applications, asparaginase is also used in the food industry to reduce the formation of acrylamide, a potentially carcinogenic compound, in starchy and fried foods. The versatility of this enzyme extends to various research fields, including biotechnology and biochemistry.

References

View all 49 references: Citation Explorer

Monitoring asparaginase activity to improve the management of patients with acute lymphoblastic leukemia: Experience in one center.

Authors:

García Morin, Marina and Melero Guardia, Paula and Bardón-Cancho, Eduardo J and Zapico Muñiz, Edgar and Cela, Elena

Journal:

Anales de pediatria (2024): 65-66

Determination of l-Asparaginase Activity and Its Therapeutic Monitoring in Children with Hematological Malignancies in a Single Croatian Center.

Authors:

Lenicek Krleza, Jasna and Katusic Bojanac, Ana and Jakovljevic, Gordana

Journal:

Diagnostics (Basel, Switzerland) (2024)

Improved HPLC method with automated pre-column sample derivatisation for serum pegylated L-asparaginase activity measurement in paediatric acute lymphoblastic leukaemia patients.

Authors:

Tan, Yan Qi and Loh, C-Khai and Mohd Saffian, Shamin and Makpol, Suzana

Journal:

Journal of pharmaceutical and biomedical analysis (2024): 116243

Allergic Reactions to E. coli Asparaginase are Associated with Decreased Asparaginase Activity in an Indonesian Pediatric Population with ALL.

Authors:

Sari, Nur Melani and Berbudi, Afiat and Susanah, Susi and Reniarti, Lelani and Supriyadi, Eddy and Kaspers, Gertjan J L and Buddington, Randal K and Howard, Scott and Idjradinata, Ponpon

Journal:

Asian Pacific journal of cancer prevention : APJCP (2023): 2773-2780

Our Experiences with Asparaginase Activity Measurements in Children with Lymphoblastic Diseases.

Authors:

Müller, Judit and Egyed, Petra and Erdelyi, Daniel and Kovacs, Krisztian and Mudra, Katalin and Szabo, Sandor and Egyed, Balint and Gabor, Kovacs

Journal:

Children (Basel, Switzerland) (2023)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 24, 2026

Component A: Amplite® Red Substrate	1 Vial
Component B1: Enzyme Mix 1	1 Bottle (lyophilized powder)
Component B2: Enzyme Mix 2	1 Vial (lyophilized powder)
Component C: Assay Buffer	1 Bottle (5 mL)
Component D: Conversion Mix	1 Vial (lyophilized powder)
Component E: Aspartate Standard	1 Vial
Component F: Asparaginase Positive Control	1 Vial (lyophilized powder)
Component G: DMSO	1 Vial (100 µL)