Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit

Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit is a fluorimetric assay kit for quantification of AST activity in various biological samples.

Detection Principle: Measures L-glutamate generated by the AST-catalyzed reaction.
Sensitivity: Detects AST activity down to 2 mU/mL.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.
Workflow: Compatible with 96- and 384-well microplates.

AST dose response was measured with Amplite® Fluorimetric Aspartate Aminotransferase Assay Kit in a 96-well black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13800	200 Tests	Price

Overview

Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family. It catalyzes the reversible transfer of an alpha-amino group between aspartate and glutamate, and is an important enzyme in amino acid metabolism. AST is found in many body tissues such as liver, heart, muscle, kidneys, brain. In healthy subjects, serum AST levels are low. However, when cells are damaged, such as acute and chronic hepatitis, obstructive jaundice, carcinoma of liver, myocardial infarction, AST may leak into the blood stream and the AST levels are significantly elevated. Therefore, determination of serum AST level has great clinical and diagnostic significance. Amplite® Fluorimetric Aspartate Aminotransferase (AST) assay kit provides a quick and sensitive method for the measurement of AST in various biological samples. Aspartate transaminase catalyzes the reaction of aspartate and α-ketoglutarate to oxaloacetate and glutamate. The product L-glutamate is measured by the generation of a red fluorescent product through an enzyme coupled reaction cycle. The signal can be read by a fluorescence microplate reader. With the Amplite® Fluorimetric Aspartate Aminotransferase Assay Kit, we have detected as little as 2 mU/mL AST in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.

Citations

View all 5 citations: Citation Explorer

Reparative Efficacy of Liposome-Encapsulated Oleanolic Acid against Liver Inflammation Induced by Fine Ambient Particulate Matter and Alcohol in Mice

Authors:

Wei, Ching-Ting and Wang, Yu-Wen and Wu, Yu-Chiuan and Lin, Li-Wei and Chen, Chia-Chi and Chen, Chun-Yin and Kuo, Shyh-Ming

Journal:

Pharmaceutics (2022): 1108

The receptor for advanced glycation endproducts (RAGE) contributes to severe inflammatory liver injury in mice

Authors:

Weinhage, Toni and Wirth, Timo and Sch{\"u}tz, Paula and Becker, Philipp and Lueken, Aloys and Skryabin, Boris V and Wittkowski, Helmut and Foell, Dirk

Journal:

Frontiers in immunology (2020): 1157

Reparative effects of astaxanthin-hyaluronan nanoaggregates against retrorsine-CCl4-induced liver fibrosis and necrosis

Authors:

Wu, Yi Jhen and Wu, Yu Chiuan and Chen, I-Fen and Wu, Yi-Lung and Chuang, Chin Wen and Huang, Han Hsiang and Kuo, Shyh Ming

Journal:

Molecules (2018): 726

Regulation of HGF-induced hepatocyte proliferation by the small GTPase Arf6 through the PIP 2-producing enzyme PIP5K1A

Authors:

Tsai, Meng-Tsz and Katagiri, Naohiro and Ohbayashi, Norihiko and Iwasaki, Kenichi and Ohkohchi, Nobuhiro and Ding, Shih-Torng and Kanaho, Yasunori and Funakoshi, Yuji

Journal:

Scientific reports (2017): 1--12

In vitro and in vivo study of the application of volvox spheres to co-culture vehicles in liver tissue engineering

Authors:

Chang, Siou Han and Huang, Han Hsiang and Kang, Pei Leun and Wu, Yu Chian and Chang, Ming-Huang and Kuo, Shyh Ming

Journal:

Acta Biomaterialia (2017)

References

View all 27 references: Citation Explorer

Fundus fluorescein angiography of choroidal tubercles: Case reports and review of literature

Authors:

Mehta S., undefined

Journal:

Indian J Ophthalmol (2006): 273

Safety of sodium fluorescein for in vivo study of skin

Authors:

O'Goshi K, Serup J.

Journal:

Skin Res Technol (2006): 155

Delayed absence seizure: A complication of intrathecal fluorescein injection A case report and literature review

Authors:

Anari S, Waldron M, Carrie S.

Journal:

Auris Nasus Larynx. (2006)

Fluorescein angiography and adverse drug reactions revisited: the Lions Eye experience

Authors:

Kwan AS, Barry C, McAllister IL, Constable I.

Journal:

Clin Experiment Ophthalmol (2006): 33

Methods of digital differential fluorescein study of the ocular drainage tract

Authors:

Shmyreva VF, Petrov S, Novikov IA, Zueva Iu S.

Journal:

Vestn Oftalmol (2005): 3

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: AST Enzyme Mixture	1 bottle (lyophilized powder)
Component B: AST Enzyme Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: AST Positive Control	1 vial (10 U, lyophilized powder)