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Amplite® Fluorimetric Beta-Galactosidase Assay Kit
Green Fluorescence
E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies in this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of ?-galactosidase per cell. This kit uses a fluorogenic galactosidase substrate that can sensitively distinguish LacZ+ vs. LacZ-cells. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. The galactosidase-cleaved product has an emission spectra that can be detected with most of fluorescence instruments equipped with a FITC filter set.
β-galactosidase dose response was measured with Amplite™ Fluorimetric beta-Galactosidase Assay Kit in a Costar solid black 96-well plate using Gemini fluorescence microplate reader (Molecular Devices). 
β-galactosidase dose response was measured with Amplite™ Fluorimetric beta-Galactosidase Assay Kit in a Costar solid black 96-well plate using Gemini fluorescence microplate reader (Molecular Devices). 
CatalogSize
Price
Quantity
12601500 Tests
Price
 
Spectral properties

Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
0.95
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation490 nm
Emission525 nm
Cutoff515 nm
Recommended plateSolid black
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Page updated on September 25, 2025