Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Beta-Galactosidase Assay Kit

Green Fluorescence

Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence* is a fluorimetric assay kit for detection of beta-galactosidase activity in cells and ELISA-type assay systems.

Detection Principle: Detects beta-galactosidase cleavage of a fluorogenic galactoside substrate.
Signal Advantage: Distinguishes LacZ+ from LacZ− cells.
Detection Chemistry: Uses fluorescein di-β-D-galactopyranoside (FDG).
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm with a microplate reader.
Applications: Supports LacZ gene expression monitoring and galactosidase-conjugate detection.

&beta;-galactosidase dose response was measured with Amplite&trade; Fluorimetric beta-Galactosidase Assay Kit in a Costar solid black 96-well plate using Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
12601	500 Tests	Price

Overview

E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies in this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of β-galactosidase per cell. This kit uses a fluorogenic galactosidase substrate that can sensitively distinguish LacZ+ vs. LacZ-cells. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. The galactosidase-cleaved product has an emission spectra that can be detected with most of fluorescence instruments equipped with a FITC filter set.

References

View all 104 references: Citation Explorer

One-step high-throughput assay for quantitative detection of beta-galactosidase activity in intact gram-negative bacteria, yeast, and mammalian cells

Authors:

Vidal-Aroca F, Giannattasio M, Brunelli E, Vezzoli A, Plevani P, Muzi-Falconi M, Bertoni G.

Journal:

Biotechniques (2006): 433

A homogeneous cell-based assay to measure nuclear translocation using beta-galactosidase enzyme fragment complementation

Authors:

Fung P, Peng K, Kobel P, Dotimas H, Kauffman L, Olson K, Eglen RM.

Journal:

Assay Drug Dev Technol (2006): 263

Microfluidic sequential injection analysis in a short capillary

Authors:

Du WB, Fang Q, Fang ZL.

Journal:

Anal Chem (2006): 6404

Use of monoclonal antibodies to quantify the dynamics of alpha-galactosidase and endo-1,4-beta-glucanase production by Trichoderma hamatum during saprotrophic growth and sporulation in peat

Authors:

Thornton CR., undefined

Journal:

Environ Microbiol (2005): 737

Identification of bacteria with beta-galactosidase activity in faeces from lactase non-persistent subjects

Authors:

He T, Priebe MG, Vonk RJ, Welling GW.

Journal:

FEMS Microbiol Ecol (2005): 463

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Fluorescein di-β-D-Galactopyranoside (FDG)	1 vial
Component B: Reaction Buffer	1 bottle (50 mL)
Component C: Stop Buffer	1 vial (25 mL)
Component D: Lysis Buffer	1 vial (25 mL)
Component E: DMSO	1 vial (500 µL)
Component F: β-Mercaptoethanol	1 vial (500 µL)