Name: Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit
Brand: AAT Bioquest
SKU: 11407
Price: 594 USD
Availability: InStock

Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit is a fluorimetric assay kit for quantification of butyrylcholinesterase activity in a variety of samples.

Detection Principle: Quantifies thiocholine generated from butyrylthiocholine hydrolysis.
Sensitivity: Detects BChE activity down to 0.1 mU/mL.
Detection Chemistry: Uses Thiolite™ Green for thiol-sensitive fluorescence detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm.
Applications: Supports BChE activity measurement in diverse sample types.

Butyrylcholinesterase dose response was measured in a solid black bottom 96-well plate with Amplite® Fluorimetric Butyrylcholinesterase Assay Kit using a Gemini fluorescence microplate reader. As low as 0.1 mU/mL of Butyrylcholinesterase can be detected with 10 minutes incubation.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11407	100 Tests	Price

Overview

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood. BChE is a nonspecific cholinesterase enzyme and can hydrolyze many different choline esters, serving as the first line of defense against toxic compounds reaching the bloodstream. It has been identified as a biomarker of organophosphate poisoning. Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit uses our outstanding Thiolite™ Green to quantify the generation of thiolcholine produced from the hydrolysis of butyrylthiocholine by BChE. The fluorescence intensity of Thiolite Green™ is proportional to the formation of thiolcholine, thus the BChE activity. The assay is convenient, sensitive and can detect as low as 0.1mU/mL in variety of samples.

Citations

View all 35 citations: Citation Explorer

Thiol-ene click reaction-induced fluorescence enhancement by altering the radiative rate for assaying butyrylcholinesterase activity

Authors:

Chen, G., Feng, H., Xi, W., Xu, J., Pan, S., Qian, Z.

Journal:

Analyst (2019): 559-566

Profiling Auspicious Butyrylcholinesterase Inhibitory Activity of Two Herbal Molecules: Hyperforin and Hyuganin C

Authors:

Orhan, I. E., Senol Deniz, F. S., Traedal-Henden, S., Ceron-Carrasco, J. P., den Haan, H., Pena-Garcia, J., Perez-Sanchez, H., Emerce, E., Skalicka-Wozniak, K.

Journal:

Chem Biodivers (2019): e1900017

Haplotypes of butyrylcholinesterase K-variant and their influence on the enzyme activity

Authors:

Jasiecki, J., Zuk, M., Krawczynska, N., Jonca, J., Szczoczarz, A., Lew and owski, K., Waleron, K., Wasag, B.

Journal:

Chem Biol Interact (2019): 154-157

Discovery of New Selective Butyrylcholinesterase (BChE) Inhibitors with Anti-Abeta Aggregation Activity: Structure-Based Virtual Screening, Hit Optimization and Biological Evaluation

Authors:

Jiang, C. S., Ge, Y. X., Cheng, Z. Q., Wang, Y. Y., Tao, H. R., Zhu, K., Zhang, H.

Journal:

Molecules (2019): se name="11406.enl" path="C:\Users\aatbi\Drop

Drop of Butyrylcholinesterase Activity after Cyclophosphamide Conditioning as a Predictive Marker of Liver Transplant-Related Complications and Its Correlation with Transplant-Related Mortality in Pediatric Hematopoietic Stem Cell Recipients

Authors:

Maximova, N., Caddeo, G., Zanon, D., Maestro, A., Simeone, R.

Journal:

J Clin Med (2019): se name="11406.enl" path="C:\Users\aatbi\Drop

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 5, 2026

Component A: Thiolite™ Green	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Butyrylthiocholine	1 vial
Component D: Butyrylcholinesterase Standard	1 vial (0.25 U)
Component E: DMSO	1 vial (100 uL)