Name: Amplite® Fluorimetric cADP-Ribose Assay Kit
Brand: AAT Bioquest
SKU: 20305
Price: 594 USD
Availability: InStock

Amplite® Fluorimetric cADP-Ribose Assay Kit

Amplite® Fluorimetric cADP-Ribose Assay Kit is a fluorimetric assay kit for quantification of cADP-ribose in tissues and cell cultures.

Detection Principle: Converts cADPR to NAD+ for fluorescent detection.
Sensitivity: Detects cADPR down to 100 nM.
Specificity: Quest Fluor™ NAD reagent responds to NAD+ but not NADH.
Instrument Compatibility: Fluorescence detection at Ex/Em 420/480 nm.
Plate Format: Compatible with 96- and 384-well microplates.

The concentration of cADPR was detected using Amplite® Fluorimetric cADP-Ribose Assay Kit. Different concentrations of cADPR were incubated with ADPRC reaction mix for 20 min at RT before NAD detection reagent was added. The lowest detected concentration of cADPR is 100 nM.

Protocol

SDS

Catalog	Size	Price	Quantity
20305	100 tests	Price

Overview

cADP-ribose (cADPR) is a novel Ca2+ messenger derived from NAD+. ADP-riboxyl cyclase (ADPRC) catalyzes the synthesis of cADPR from NAD+, but the reaction can be reversed in the presence of high concentration of nicotinamide, producing NAD+ from cADPR stoichiometrically. The resultant NAD+ can be detected using our newly developed NAD sensor Quest Fluor™ NAD reagent. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range. The NAD+ detection using Quest Fluor™ NAD reagent is specific to NAD+ and has no reaction to NADH. The fluorescent signal can be readily detected. This assay can be performed in a convenient 96-well or 384-well microtiter plate.

Citations

View all 4 citations: Citation Explorer

Targeting the SARM1--cADPR--Ca$^2$⁺ Pathway Attenuates Mitochondrial Fragmentation and Osteoarthritis Progression

Authors:

Gu, Yu and Zhao, Xin-Hao and Xing, Bai-Zhou and Wu, Biao and Peng, Rong and Li, Chang-Qing and Lu, Hua-Ding and others,

Altered DNA methylation underlies monocyte dysregulation and immune exhaustion memory in sepsis

Authors:

Caldwell, Blake A and Wu, Yajun and Wang, Jing and Li, Liwu

Journal:

Cell Reports (2024)

Exploring the role of NAD metabolism in response to mitochondrial respiratory chain inhibition

Authors:

Sarkar, Ankita

Early loss of endogenous NAD+ following rotenone treatment leads to mitochondrial dysfunction and Sarm1 induction that is ameliorated by PARP inhibition

Authors:

Sarkar, Ankita and Dutta, Sourav and Sur, Malinki and Chakraborty, Semanti and Dey, Puja and Mukherjee, Piyali

Journal:

The FEBS Journal (2022)

References

View all 35 references: Citation Explorer

Ca 2+ release induced by cADP-ribose is mediated by FKBP12. 6 proteins in mouse bladder smooth muscle

Authors:

Zheng, Ji and Wenzhi, Bi and Miao, Lin and Hao, Yumin and Zhang, Xu and Yin, Wenxuan and Pan, Jinhong and Yuan, Zengqiang and Song, Bo and Ji, Guangju

Journal:

Cell calcium (2010): 449--457

ADP-ribose and cADP-ribose--endogenous regulators of cellular ionic balance. Cardiotropic effects of ADP-ribose

Authors:

Kuz'min, VS and Sosulina, LIu and Sukhova, GS and Ashmarin, IP

Journal:

Uspekhi fiziologicheskikh nauk (2006): 3--17

Ryanodine receptor subtype 2 encodes Ca2+ oscillations activated by acetylcholine via the M2 muscarinic receptor/cADP-ribose signalling pathway in duodenum myocytes

Authors:

Fritz, Nicolas and Macrez, Nathalie and Mironneau, Jean and Jeyakumar, Loice H and Fleischer, Sidney and Morel, Jean-Luc

Journal:

Journal of cell science (2005): 2261--2270

Modulation of spontaneous transient Ca 2+-activated K+ channel currents by cADP-ribose in vascular smooth muscle cells

Authors:

Cheung, Donald W

Journal:

European journal of pharmacology (2003): 57--59

A novel cycling assay for cellular cADP-ribose with nanomolar sensitivity

Authors:

Graeff, Richard and Lee, Hon Cheung

Journal:

Biochemical Journal (2002): 379--384

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	420 nm
Emission	480 nm
Cutoff	435 nm
Recommended plate	Solid black

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Page updated on October 6, 2026

Component A: Quest Fluor™ NAD Probe	1 bottle (5 mL)
Component B: ADPRC Enzyme mix	1 vial
Component C: Assay Solution I	1 bottle (12 mL)
Component D: Assay Solution II	1 bottle (5 mL)
Component E: Enhancer Solution	1 bottle (3.5 mL)
Component F: cADPR Standard	1 vial