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Amplite® Fluorimetric cADP-Ribose Assay Kit
cADP-ribose (cADPR) is a novel Ca2+ messenger derived from NAD+. ADP-riboxyl cyclase (ADPRC) catalyzes the synthesis of cADPR from NAD+, but the reaction can be reversed in the presence of high concentration of nicotinamide, producing NAD+ from cADPR stoichiometrically. The resultant NAD+ can be detected using our newly developed NAD sensor Quest Fluor™ NAD reagent. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range. The NAD+ detection using Quest Fluor™ NAD reagent is specific to NAD+ and has no reaction to NADH. The fluorescent signal can be readily detected. This assay can be performed in a convenient 96-well or 384-well microtiter plate.
The concentration of cADPR was detected using Amplite® Fluorimetric cADP-Ribose Assay Kit. Different concentrations of cADPR were incubated with ADPRC reaction mix for 20 min at RT before NAD detection reagent was added. The lowest detected concentration of cADPR is 100 nM.
The concentration of cADPR was detected using Amplite® Fluorimetric cADP-Ribose Assay Kit. Different concentrations of cADPR were incubated with ADPRC reaction mix for 20 min at RT before NAD detection reagent was added. The lowest detected concentration of cADPR is 100 nM.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
20305100 tests
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation420 nm
Emission480 nm
Cutoff435 nm
Recommended plateSolid black
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Page updated on September 25, 2025