Name: Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 36360
Price: 237 USD
Availability: InStock

Amplite® Fluorimetric Calcium Quantitation Kit

Red Fluorescence

Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence* is a fluorimetric assay kit for detecting calcium in physiological solutions.

Detection Principle: Uses a proprietary red fluorescent calcium probe.
Sensitivity: Detects calcium down to 0.03 mM.
Detection Range: Measures calcium from 30 µM to 10 mM.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.
Workflow: Compatible with 96- and 384-well microplates, or can be adapted to automation without purification.

Calcium dose response was measured on a solid black 96-well plate with Amplite® Fluorimetric Calcium Quantitation Kit.

Protocol

SDS

Catalog	Size	Price	Quantity
36360	200 Tests	Price

Overview

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca2+ into and out of the cytoplasm functions as a signal for many cellular processes. Calcium is the fifth most abundant element by mass in the human body, where it is a common cellular ionic messenger with many functions, and serves also as a structural element in bone. Calcium plays an important role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. The serum level of calcium is closely regulated within a fairly limited range (9 to 10.5 mg/dL) in the human body. Both hypocalcaemia and hypercal caemia are serious medical disorders. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. Amplite® Calcium Detection Kit using our proprietary red fluorescence probe provides a simple method for detecting calcium in physiology solutions. The fluorescent signal can be easily read by fluorescence microplate reader. The Amplite® Calcium Detection Kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required. The assay can be completed within 30 minutes. With the Amplite® Calcium Detection Kit, we have detected as little as 0.03 mM calcium.

Citations

View all 6 citations: Citation Explorer

Ca2+ transients on the T cell surface trigger rapid integrin activation in a timescale of seconds

Authors:

Li, Yue and Wang, ShiHui and Zhang, YouHua and Liu, ZhaoYuan and Zheng, YunZhe and Zhang, Kun and Chen, ShiYang and Lv, XiaoYing and Huang, MengWen and Pan, XingChao and others,

Journal:

Nature Communications (2024): 1--16

The CLOCK protein regulates insulin secretion related with L-type calcium channels in rat pancreatic beta cells

Authors:

Tian, Linlin and Li, Xiaodong and Ding, Yi and Li, Minli and Tang, Yunzhao and Li, Daiqing

Journal:

Biochemical and biophysical research communications (2022): 116--122

Effects of Eggshell Membrane on Keratinocyte Differentiation and Skin Aging In Vitro and In Vivo

Authors:

Furukawa, Kyohei and Kono, Masaya and Kataoka, Tetsuro and Hasebe, Yukio and Jia, Huijuan and Kato, Hisanori

Journal:

Nutrients (2021): 2144

Realgar transforming solution as a novel arsenic agent with a lower risk of cardiotoxicity

Authors:

Hai, Yang and Song, Peng and Wang, Xin and Zhao, Longhe and Xie, Qinjian and Li, Jianyin and Li, Yang and Li, Hongyu

Journal:

Journal of pharmacological sciences (2019): 162--170

Microcystin-LR causes sexual hormone disturbance in male rat by targeting gonadotropin-releasing hormone neurons

Authors:

Wang, Xueting and Ding, Jie and Xiang, Zou and Jiang, Peipei and Du, Jing and Han, Xiaodong

Journal:

Toxicon (2016): 45--55

References

View all 105 references: Citation Explorer

Calcium, magnesium, iron, zinc and copper concentration in the hair of tobacco smokers

Authors:

Unkiewicz-Winiarczyk A, Bagniuk A, Gromysz-Kalkowska K, Szubartowska E.

Journal:

Biol Trace Elem Res (2009): 152

Genetic and nongenetic variation in concentration of selenium, calcium, potassium, zinc, magnesium, and phosphorus in milk of Dutch Holstein-Friesian cows

Authors:

van Hulzen KJ, Sprong RC, van der Meer R, van Arendonk JA.

Journal:

J Dairy Sci (2009): 5754

Comparison of calcium, magnesium, sodium, potassium, zinc, and creatinine concentration in 24-h and spot urine samples in women

Authors:

Ilich JZ, Blanusa M, Orlic ZC, Orct T, Kostial K.

Journal:

Clin Chem Lab Med (2009): 216

Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators

Authors:

Spano JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pecora JD.

Journal:

J Endod (2009): 727

3,4-Dichloropropionanilide (DCPA) inhibits T-cell activation by altering the intracellular calcium concentration following store depletion

Authors:

Lewis TL, Brundage KM, Brundage RA, Barnett JB.

Journal:

Toxicol Sci (2008): 97

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 4, 2026

Component A: Rhod Red™ Indicator (light sensitive)	2 vials
Component B: Assay Buffer	1 bottle (10 mL)
Component C: 300 mM Calcium Standard	1 vial (0.5 mL)