Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Caspase 3/7 Assay Kit

Blue Fluorescence

Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence* is a fluorimetric assay kit for quantification of caspase 3/7 activity in cell extracts and purified enzyme preparations.

Detection Principle: Detects caspase-mediated cleavage of a fluorogenic DEVD substrate.
Detection Chemistry: Uses Ac-DEVD-AMC as the caspase 3/7 substrate.
Instrument Compatibility: Fluorescence detection at Ex/Em 350/450 nm.
Applications: Supports apoptosis studies and caspase 3/7 activity analysis.

Detection of Caspase 3/7 activity in Jurkat cells with Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit. Jurkat cells were seeded on the same day at 80,000 cells/well/90 µL in a Costar black wall/clear bottom 96-well plate. The cells were treated with or without 1 µM of staurosporine for 4 hours, and with or without 10 µM of the caspase inhibitor AC-DEVD-CHO for 10 minutes. The caspase 3/7 assay solution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 350/450 nm (Cutoff = 420 nm).

Protocol

SDS

Catalog	Size	Price	Quantity
13502	500 Tests	Price

Overview

Caspases play important roles in apoptosis and cell signaling. The activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 is also identified as a drug-screening target. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This Amplite® Caspase-3 Assay Kit uses Ac-DEVD-AMC as fluorogenic indicator for assaying caspase-3 activity. AMC-derived caspase substrates are widely used for fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm. This kit can be used to continuously measure the activities of caspase-3 in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer.

Citations

View all 24 citations: Citation Explorer

BRPF1 inhibition reduces migration and invasion of metastatic ovarian cancer cells, representing a potential therapeutic target

Authors:

Alexandrova, Elena and Smal, Marharyta and Di Rosa, Domenico and Brancaccio, Rosario Nicola and Parisi, Roberto and Russo, Fabio and Tarallo, Roberta and Nassa, Giovanni and Giurato, Giorgio and Weisz, Alessandro and others,

Journal:

Scientific Reports (2025): 7602

An engineered Palivizumab IgG2 subclass for synthetic gp130 and fas mediated signaling

Authors:

Wittich, Christoph and Ettich, Julia and Hertell, Marcel and Roy, Biswadeep G and Xu, Haifeng C and Floss, Doreen M and Lang, Philipp A and Scheller, J{\"u}rgen

Journal:

Journal of Biological Chemistry (2025): 108205

Goji Berry Juice Prevents Tumor Necrosis Factor Alpha-Induced Xerostomia in Human Salivary Gland Cells

Authors:

Takakura, Masatoshi and Mizutani, Ayano and Kudo, Mizuki and Ishikawa, Airi and Okamoto, Takuya and Fu, Tong Xuan and Kurimoto, Shin-ichiro and Koike, Yuka and Mishima, Kenji and Tanaka, Junichi and others,

Journal:

Biological and Pharmaceutical Bulletin (2024): 138--144

Respiratory syncytial virus--approved mAb Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors

Authors:

Ettich, Julia and Wittich, Christoph and Moll, Jens M and Behnke, Kristina and Floss, Doreen M and Reiners, Jens and Christmann, Andreas and Lang, Philipp A and Smits, Sander HJ and Kolmar, Harald and others,

Journal:

The Journal of Biological Chemistry (2023)

Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors

Authors:

Journal:

Journal of Biological Chemistry (2023): 105270

References

View all 67 references: Citation Explorer

In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline. Involvement of caspase cascades and IkappaBalpha phosphorylation

Authors:

Lerma-Diaz JM, Hern and ez-Flores G, Dominguez-Rodriguez JR, Ortiz-Lazareno PC, Gomez-Contreras P, Cervantes-Munguia R, Scott-Algara D, Aguilar-Lemarroy A, Jave-Suarez LF, Bravo-Cuellar A.

Journal:

Immunol Lett (2006): 149

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	350 nm
Emission	450 nm
Cutoff	420 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Caspase 3/7 Substrate (200X Stock Solution)	1 vial (250 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: DTT (1M)	1 vial (600 µL)
Component D: Ac-DEVD-CHO (Caspase 3/7 Inhibitor)	1 vial