Name: Amplite® Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 13503
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric Caspase 3/7 Assay Kit

Green Fluorescence

Amplite® Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence* is a fluorimetric assay kit for quantification of caspase 3/7 activity in cell extracts and purified enzyme preparations.

Detection Principle: Detects caspase cleavage of a DEVD-based fluorogenic substrate.
Signal Advantage: R110-based substrate provides sensitive caspase activity detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 490/525 nm.
Applications: Supports apoptosis analysis and caspase 3/7 activity studies.

Detection of caspase 3/7 Activity in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/well/90 µL in a black wall/clear bottom 96-well costar plate. The cells were treated with or without 20 µM ofcamptothecin for 5 hours, and/or 5 µM caspase 3/7 inhibitor AC-DEVD-CHO for 10 minutes. The caspase 3/7 assaysolution (100 µL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity wasmeasured at Ex/Em = 490/525 nm using the NOVOstar instrument (BMG Labtech).

Protocol

SDS

Catalog	Size	Price	Quantity
13503	500 Tests	Price

Overview

Caspases play important roles in apoptosis and cell signaling. The activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 is also identified as a drug-screening target. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This Amplite® Caspase-3 Assay Kit uses (Z-DEVD)2R110 as fluorogenic indicator for assaying caspase-3 activity. Cleavage of R110 peptides by caspases generates strongly fluorescent R110 that can be monitored fluorimetrically at 510-530 nm with excitation of 488 nm, the most common excitation light source used in fluorescence instruments. R110-derived caspase substrates are probably the most sensitive indicators widely used for the fluorimetric detection of various caspase activities. This kit can be used to continuously measure the activities of caspase-3 in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It can also be used with flow cytometry for analyzing cell apoptosis and the activities of caspases 3 and 7.

Citations

View all 25 citations: Citation Explorer

Multifunctional and Scalable Nanoparticles for Bimodal Image-Guided Phototherapy in Bladder Cancer Treatment

Authors:

Tang, Menghuan and Mahri, Sohaib and Shiau, Ya-Ping and Mukarrama, Tasneem and Villa, Rodolfo and Zong, Qiufang and Racacho, Kelsey Jane and Li, Yangxiong and Lee, Yunyoung and Huang, Yanyu and others,

Journal:

Nano-Micro Letters (2025): 222

BRPF1 inhibition reduces migration and invasion of metastatic ovarian cancer cells, representing a potential therapeutic target

Authors:

Alexandrova, Elena and Smal, Marharyta and Di Rosa, Domenico and Brancaccio, Rosario Nicola and Parisi, Roberto and Russo, Fabio and Tarallo, Roberta and Nassa, Giovanni and Giurato, Giorgio and Weisz, Alessandro and others,

Journal:

Scientific Reports (2025): 7602

An engineered Palivizumab IgG2 subclass for synthetic gp130 and fas mediated signaling

Authors:

Wittich, Christoph and Ettich, Julia and Hertell, Marcel and Roy, Biswadeep G and Xu, Haifeng C and Floss, Doreen M and Lang, Philipp A and Scheller, J{\"u}rgen

Journal:

Journal of Biological Chemistry (2025): 108205

Goji Berry Juice Prevents Tumor Necrosis Factor Alpha-Induced Xerostomia in Human Salivary Gland Cells

Authors:

Takakura, Masatoshi and Mizutani, Ayano and Kudo, Mizuki and Ishikawa, Airi and Okamoto, Takuya and Fu, Tong Xuan and Kurimoto, Shin-ichiro and Koike, Yuka and Mishima, Kenji and Tanaka, Junichi and others,

Journal:

Biological and Pharmaceutical Bulletin (2024): 138--144

Respiratory syncytial virus--approved mAb Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors

Authors:

Ettich, Julia and Wittich, Christoph and Moll, Jens M and Behnke, Kristina and Floss, Doreen M and Reiners, Jens and Christmann, Andreas and Lang, Philipp A and Smits, Sander HJ and Kolmar, Harald and others,

Journal:

The Journal of Biological Chemistry (2023)

References

View all 67 references: Citation Explorer

Multiparameter measurement of caspase 3 activation and apoptotic cell death in NT2 neuronal precursor cells using high-content analysis

Authors:

Fennell M, Chan H, Wood A.

Journal:

J Biomol Screen (2006): 296

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Asymmetric dimethylarginine induces apoptosis via p38 MAPK/caspase-3-dependent signaling pathway in endothelial cells

Authors:

Jiang DJ, Jia SJ, Dai Z, Li YJ.

Journal:

J Mol Cell Cardiol (2006): 529

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	80000
Excitation (nm)	500
Emission (nm)	522

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Fax
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Page updated on October 8, 2026

Component A: Caspase 3/7 Substrate (200X Stock Solution)	1 vial (250 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: DTT	1 vial (600 µL, 1M)
Component D: Ac-DEVD-CHO (Caspase 3/7 Inhibitor)	1 vial
Component E: R110 Standard	1 vial (50 µL, 5 mM)