Name: Amplite® Fluorimetric Caspase 3/7 Assay Kit *Red Fluorescence*
Brand: AAT Bioquest
SKU: 13504
Price: 415 USD
Availability: InStock

Amplite® Fluorimetric Caspase 3/7 Assay Kit

Red Fluorescence

Amplite® Fluorimetric Caspase 3/7 Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of caspase 3/7 activity in cell extracts and purified enzyme preparations.

Detection Principle: Detects caspase cleavage of a DEVD-based fluorogenic substrate.
Signal Advantage: Produces strong red fluorescence for sensitive caspase 3/7 detection.
Instrument Compatibility: Fluorescence detection at Ex/Em 535/620 nm.
Applications: Supports apoptosis analysis by microplate reader, fluorometer, or flow cytometry.

Detection of Caspase 3/7 Activities in Jurkat cells. Jurkat cells were seeded on the same day at 200,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with staurosporine at the final concentration of 1 uM for 5 hours while the untreated cells were used as control. The caspase 3/7 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm with FlexStation fluorescence microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13504	100 tests	Price

Overview

Caspases play important roles in apoptosis and cell signaling. The activation of caspase-3 (CPP32/apopain) is important for the initiation of apoptosis. Caspase 3 is also identified as a drug-screening target. Caspase 3 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). This Amplite® Caspase-3 Assay Kit uses Z-DEVD-ProRed™ as the fluorogenic indicator for assaying caspase-3 activity. Cleavage of R110 peptides by caspases generates strongly red fluorescent ProRed™ that can be monitored fluorimetrically at ~620 nm with excitation of ~530 nm. Z-DEVD-ProRed™ is recognized as the most sensitive red fluorogenic caspase 3/7 substrate. This kit can be used to continuously measure the activities of caspase-3 in cell extracts and purified enzyme preparations using a fluorescence microplate reader or fluorometer. It can also be used with flow cytometry for analyzing cell apoptosis and the activities of caspases 3 and 7.

Citations

View all 18 citations: Citation Explorer

BRPF1 inhibition reduces migration and invasion of metastatic ovarian cancer cells, representing a potential therapeutic target

Authors:

Alexandrova, Elena and Smal, Marharyta and Di Rosa, Domenico and Brancaccio, Rosario Nicola and Parisi, Roberto and Russo, Fabio and Tarallo, Roberta and Nassa, Giovanni and Giurato, Giorgio and Weisz, Alessandro and others,

Journal:

Scientific Reports (2025): 7602

An engineered Palivizumab IgG2 subclass for synthetic gp130 and fas mediated signaling

Authors:

Wittich, Christoph and Ettich, Julia and Hertell, Marcel and Roy, Biswadeep G and Xu, Haifeng C and Floss, Doreen M and Lang, Philipp A and Scheller, J{\"u}rgen

Journal:

Journal of Biological Chemistry (2025): 108205

Goji Berry Juice Prevents Tumor Necrosis Factor Alpha-Induced Xerostomia in Human Salivary Gland Cells

Authors:

Takakura, Masatoshi and Mizutani, Ayano and Kudo, Mizuki and Ishikawa, Airi and Okamoto, Takuya and Fu, Tong Xuan and Kurimoto, Shin-ichiro and Koike, Yuka and Mishima, Kenji and Tanaka, Junichi and others,

Journal:

Biological and Pharmaceutical Bulletin (2024): 138--144

Respiratory syncytial virus--approved mAb Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors

Authors:

Ettich, Julia and Wittich, Christoph and Moll, Jens M and Behnke, Kristina and Floss, Doreen M and Reiners, Jens and Christmann, Andreas and Lang, Philipp A and Smits, Sander HJ and Kolmar, Harald and others,

Journal:

The Journal of Biological Chemistry (2023)

Respiratory syncytial virus (RSV)-approved monoclonal antibody Palivizumab as ligand for anti-idiotype nanobody-based synthetic cytokine receptors

Authors:

Journal:

Journal of Biological Chemistry (2023): 105270

References

View all 67 references: Citation Explorer

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Serofendic acid, a neuroprotective substance derived from fetal calf serum, inhibits mitochondrial membrane depolarization and caspase-3 activation

Authors:

Kume T, Taguchi R, Katsuki H, Akao M, Sugimoto H, Kaneko S, Akaike A.

Journal:

Eur J Pharmacol (2006): 69

Multiparameter measurement of caspase 3 activation and apoptotic cell death in NT2 neuronal precursor cells using high-content analysis

Authors:

Fennell M, Chan H, Wood A.

Journal:

J Biomol Screen (2006): 296

Asymmetric dimethylarginine induces apoptosis via p38 MAPK/caspase-3-dependent signaling pathway in endothelial cells

Authors:

Jiang DJ, Jia SJ, Dai Z, Li YJ.

Journal:

J Mol Cell Cardiol (2006): 529

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Spectral properties

Excitation (nm)	532
Emission (nm)	619

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	535 nm
Emission	620 nm
Cutoff	610 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
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Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 8, 2026

Component A: Z-DEVD-ProRed™	1 vial
Component B: Assay Buffer	1 bottle (10 mL)
Component C: DTT	1 vial (200 µL, 1M)
Component D: Ac-DEVD-CHO (Caspase 3/7 Inhibitor)	1 vial