Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Catalase Assay Kit

Red Fluorescence

Amplite® Fluorimetric Catalase Assay Kit *Red Fluorescence* is a fluorimetric assay kit for quantification of catalase activity in a microplate format.

Detection Principle: Measures catalase-mediated depletion of hydrogen peroxide.
Sensitivity: Detects catalase down to 30 mU/mL.
Detection Chemistry: Uses Amplite® Red and horseradish peroxidase for H₂O₂ detection.
Workflow/Instrument Compatibility: Supports 96- and 384-well plates with fluorescence or absorbance readout.

Catalase dose response was measured with Amplite® Fluorimetric Catalase Assay Kit in a 96-well black solid plate using a Gemini fluorescence microplate reader (Molecular Devices. As low as 30 mU/mL catalase can be detected with 30 minutes incubation time (n=3).

Protocol

SDS

Catalog	Size	Price	Quantity
11306	200 Tests	Price

Overview

Catalase is a common antioxidant heme-containing redox enzyme found in nearly all living organisms that are exposed to oxygen. The enzyme is concentrated in the peroxisome subcellular organelles. Hydrogen peroxide is an ROS that is a toxic product of normal aerobic metabolism and pathogenic ROS production involving oxidase and superoxide dismutase reactions. By preventing the excessive buildup of H2O2, catalase allows important cellular processes which produce H2O2 as a by-product to take place safely. The Amplite® Fluorimetric Catalase Assay Kit provides a quick and sensitive method for the measurement of catalase activity. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. Catalase reacts with H2O2 to produce water and oxygen (O2). Amplite® Red also reacts with H2O2 to generate a red fluorescent product. Therefore the reduction in fluorescence intensity is proportional to catalase activity. The Amplite® Red substrate used in the assay enables a dual recordable mode. The fluorescent signal can be easily read by either a fluorescence microplate reader or an absorbance microplate reader. With the Amplite® Fluorimetric Catalase Assay Kit, we have detected as little as 30 mU/mL catalase in a 100 µL reaction volume.

Citations

View all 7 citations: Citation Explorer

Erinacine A-Enriched Hericium erinaceus Mycelium Ethanol Extract Lessens Cellular Damage in Cell and Drosophila Models of Spinocerebellar Ataxia Type 3 by Improvement of Nrf2 Activation

Authors:

Wu, Yu-Ling and Sun, Hai-Lun and Chang, Jui-Chih and Lin, Wei-Yong and Chen, Pei-Yin and Chen, Chin-Chu and Lee, Li-Ya and Li, Chien-Chun and Hsieh, Mingli and Chen, Haw-Wen and others,

Journal:

Antioxidants (2024): 1495

Stabilizing and Prolonging the Antioxidant Activity of Catalase Using Nondegradable Nanoparticles.

Authors:

Murayama, Shuhei and Sasaki, Rikuto and Sakaguchi, Soma and Takaki, Takashi and Kato, Masaru

Journal:

Sensors \& Materials (2023)

Glutathione limits RUNX2 oxidation and degradation to regulate bone formation

Authors:

Hu, Guoli and Yu, Yilin and Sharma, Deepika and Pruett-Miller, Shondra M and Ren, Yinshi and Zhang, Guo-Fang and Karner, Courtney M

Journal:

JCI insight (2023)

A novel approach for the prevention of ionizing radiation-induced bone loss using a designer multifunctional cerium oxide nanozyme

Authors:

Wei, Fei and Neal, Craig J and Sakthivel, Tamil Selvan and Fu, Yifei and Omer, Mahmoud and Adhikary, Amitava and Ward, Samuel and Ta, Khoa Minh and Moxon, Samuel and Molinari, Marco and others,

Journal:

Bioactive materials (2023): 547--565

In Vitro Efficacy and Molecular Mechanism of Curcumin Analog in Pathological Regulation of Spinocerebellar Ataxia Type 3

Authors:

Wu, Yu-Ling and Chang, Jui-Chih and Chao, Yi-Chun and Chan, Hardy and Hsieh, Mingli and Liu, Chin-San

Journal:

Antioxidants (2022): 1389

References

View all 94 references: Citation Explorer

Screening for cytochrome p450 reactivity by harnessing catalase as reporter enzyme

Authors:

Rabe KS, Spengler M, Erkelenz M, Muller J, G and ubert VJ, Hayen H, Niemeyer CM.

Journal:

Chembiochem (2009): 751

New conjugates of superoxide dismutase/catalase mimetics with cyclodestrins

Authors:

Lanza V, Vecchio G.

Journal:

J Inorg Biochem (2009): 381

Overexpression of catalase delays G0/G1- to S-phase transition during cell cycle progression in mouse aortic endothelial cells

Authors:

Onumah OE, Jules GE, Zhao Y, Zhou L, Yang H, Guo Z.

Journal:

Free Radic Biol Med (2009): 1658

Novel homo- and hetero-nuclear copper(II) complexes of tetradentate Schiff bases: synthesis, characterization, solvent-extraction and catalase-like activity studies

Authors:

Dede B, Karipcin F, Cengiz M.

Journal:

J Hazard Mater (2009): 1148

Covalent conjugation of tetrameric bovine liver catalase to liposome membranes for stabilization of the enzyme tertiary and quaternary structures

Authors:

Yoshimoto M, Sakamoto H, Shirakami H.

Journal:

Colloids Surf B Biointerfaces (2009): 281

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Fax
Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on October 20, 2026

Component A: Amplite® Red	1 vial
Component B: H2O2	1 vial (3% stabilized solution, 200 μL)
Component C: Assay Buffer	1 bottle (50 mL)
Component D: Horseradish Peroxidase	1 vial (20 units)
Component E: Catalase Standard	1 vial (1000 U/mL, 50 μL)
Component F: DMSO	1 vial (200 μL)