Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Coenzyme A Quantitation Kit

Green Fluorescence

Coenzyme A (CoA) is a universal and essential cofactor in all forms of cellular life acting as a principal acyl carrier in numerous biosynthetic, energy-yielding, and degradative pathways. It plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation and the citric acid cycle. Measurement of CoA is one of the essential tasks for investigating biological processes and events in many biological systems. There are a few reagents or assay kits available for quantitating CoA content in biological systems. However, the existing commercial kits either lack sensitivity or have tedious procedures. Our Amplite® Fluorimetric CoA Qutitation Assay Kit provides an ultrasensitive fluorimetric assay to quantitate CoA content by detection of -SH group in CoA. Our proprietary fluorogenic CoA Green™ dye used in the kit becomes strongly fluorescent upon reacting with -SH. The assay kit can detect as little as 4 picomole of CoA in a 100 µL assay volume (40 nM). It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

CoA dose response was measured in a 96-well solid black plate with Amplite® Fluorimetric Coenzyme A Quantitation Assay Kit using a NOVOstar microplate reader (BMG Labtech).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15270	200 Tests	Price

Citations

View all 3 citations: Citation Explorer

The polyamine-regulating enzyme SSAT1 impairs tissue regulatory T cell function in chronic cutaneous inflammation

Authors:

Neuwirth, Teresa and Malzl, Daniel and Knapp, Katja and Tsokkou, Panagiota and Kleissl, Lisa and Gabriel, Anna and Reininger, Baerbel and Freyst{\"a}tter, Christian and Marella, Nara and Kutschat, Ana P and others,

Journal:

Immunity (2025)

Structural basis of the acyl-transfer mechanism of human GPAT1

Authors:

Johnson, Zachary Lee and Ammirati, Mark and Wasilko, David Jonathan and Chang, Jeanne S and Noell, Stephen and Foley, Timothy L and Yoon, Hyejin and Smith, Kathleen and Asano, Shoh and Hales, Katherine and others,

Journal:

Nature Structural \& Molecular Biology (2022): 1--9

Methods for measuring CoA and CoA derivatives in biological samples

Authors:

Tsuchiya, Yugo and Pham, Uyen and Gout, Ivan

Journal:

Biochemical Society Transactions (2014): 1107--1111

References

View all 87 references: Citation Explorer

A spectrophotometric assay for measuring acetyl-coenzyme A carboxylase

Authors:

Kroeger JK, Zarzycki J, Fuchs G.

Journal:

Anal Biochem (2011): 100

A continuous assay for alpha-methylacyl-coenzyme A racemase using circular dichroism

Authors:

Ouazia D, Bearne SL.

Journal:

Anal Biochem (2010): 45

An optimized luciferase bioluminescent assay for coenzyme A

Authors:

Marques SM, da Silva JC.

Journal:

Anal Bioanal Chem (2008): 2161

A simple homogeneous scintillation proximity assay for acyl-coenzyme A:diacylglycerol acyltransferase

Authors:

Seethala R, Peterson T, Dong J, Chu CH, Chen L, Golla R, Ma Z, Panemangalore R, Lawrence RM, Cheng D.

Journal:

Anal Biochem (2008): 144

A fluorescence-based thiol quantification assay for ultra-high-throughput screening for inhibitors of coenzyme A production

Authors:

Chung CC, Ohwaki K, Schneeweis JE, Stec E, Varnerin JP, Goudreau PN, Chang A, Cassaday J, Yang L, Yamakawa T, Kornienko O, Hodder P, Inglese J, Ferrer M, Strulovici B, Kusunoki J, Tota MR, Takagi T.

Journal:

Assay Drug Dev Technol (2008): 361

Spectral properties

Excitation (nm)	510
Emission (nm)	525

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	520 nm
Recommended plate	Solid black

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 25, 2025

Component A: CoA Green™	1 vial
Component B: Assay Buffer	1 bottle (25 mL)
Component C: Coenzyme A (CoA) Standard (FW=767.53)	1 vial (154 µg)
Component D: DMSO	1 vial (200 µL)