The Amplite® Fluorimetric Cyclooxygenase-2 (COX-2) Activity Assay kit provides a one-stop solution for measuring cyclooxygenase-2 (COX-2) activity and screening inhibitors of COX-2. Cyclooxygenase-2 is one of the two isoenzymes of cyclooxygenase, with COX-1 being constitutively expressed in many tissues. COX-2 is an inducible enzyme that converts arachidonic acid into prostaglandins, which are lipid mediators involved in inflammation, pain, and cancer. COX-2 is a key target in anti-inflammatory and anti-cancer drug research. This kit uses a fluorescence detection system to measure COX-2 activity. The enzyme catalyzes the conversion of arachidonic acid into prostaglandins, generating a fluorogenic product that results in a fluorescence signal (Ex/Em = 540/590 nm). The signal generated is directly proportional to the enzymatic activity, enabling accurate quantification. This kit provides a simple and reliable solution for detecting COX-2 activity in a wide range of biological samples, including cell lysates, serum, purified enzymes, and tissue homogenates. It is compatible with fluorescence microplate readers and is ideal for studying enzyme kinetics or disease-related pathways involving COX-2 and for screening COX-2 inhibitors.