Name: Amplite® Fluorimetric D-Lactate Assay Kit
Brand: AAT Bioquest
SKU: 13810
Price: 355 USD
Availability: InStock

Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit is a fluorimetric assay kit for detecting D-lactate in serum, plasma, urine, and cell culture samples.

Detection Principle: Uses an enzyme-coupled reaction to generate NADH.
Sensitivity: Detects D-lactate down to 1.4 µM in a 100 µL reaction.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.
Plate Format: Recommended for solid black microplates.

D-lactate dose response was measured with Amplite® Fluorimetric D-Lactate Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13810	200 Tests	Price

Overview

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid. Lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in the process of metabolism and exercise. Monitoring lactate levels is a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is not metabolized by mammals and its elimination from the body depends mainly on renal excretion. D- and L-lactic acid are found in many fermented milk products such as yogurt and cheese, and also in pickled vegetables, and cured meats and fish. The D- and L-lactic acid (generated by bacteria) is a quality indicator of foods, such as egg, milk, fruit juice and wine. Abnormal high concentration of D-lactate in the blood is usually a reflection of bacterial overgrowth in the gastrointestinal tract. AAT Bioquest's Amplite® Lactate Assay Kits (Cat# 13814 and 13815 for L-lactate assay, and Cat# 13810 and 13811 for D-lactate assay) provide both fluorescence and absorbance-based method for detecting either L-lactate or D-lactate in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, lactate is proportionally related to NADH, which is specifically monitored by a fluorogenic NADH sensor. The signal can be read by a fluorescence microplate reader. With this Fluorimetric Amplite® D-Lactate Assay Kit, we were able to detect as little as 1.4 µM D-lactate in a 100 µL reaction volume.

Citations

View all 10 citations: Citation Explorer

ROS-driven mitochondrial and mtDNA release as a novel pathway of metabolic injury in diabetes

Authors:

Chen, Dandan and Jia, Yuxin and Duan, Xiaohong and Huang, Jianhua and Deng, Xiaohong

Journal:

Journal of Molecular Endocrinology (2026): JME--25

Different sources and dosages of zinc oxide and copper influence growth performance and intestinal health of post-weaning piglets

Authors:

Negrini, Clara and Baccouri, Hadhemi and Monteiro, Alessandra and Lo Verso, Luca and Trevisi, Paolo and L{\'e}tourneau-Montminy, Marie-Pierre and Guay, Fr{\'e}d{\'e}ric

Journal:

Journal of Animal Science (2025): skaf314

A Functional Food Inhibits Azoxymethane/Dextran Sulfate Sodium-Induced Inflammatory Colorectal Cancer in Mice

Authors:

Zhang, Jie and Chen, Zhewen and Lu, Yanwen and Tu, Daoyuan and Zou, Fengqian and Lin, Shouwen and Yu, Weinan and Miao, Mingyong and Shi, Hanping

Journal:

OncoTargets and therapy (2021): 1465

NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism

Authors:

Zhang, Rufeng and Hou, Tingting and Cheng, Heping and Wang, Xianhua

Journal:

The FASEB Journal (2019): fj--201901117RR

Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating β-catenin

Authors:

Chen, Liping and Mai, Weiqian and Chen, Minfeng and Hu, Jianyang and Zhuo, Zhenjian and Lei, Xueping and Deng, Lijuan and Liu, Junshan and Yao, Nan and Huang, Maohua and others, undefined

Journal:

Pharmacological Research (2017)

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Serum lactate dehydrogenase predicts for overall survival benefit in patients with metastatic renal cell carcinoma treated with inhibition of mammalian target of rapamycin

Authors:

Armstrong AJ, George DJ, Halabi S.

Journal:

J Clin Oncol (2012): 3402

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

Adult-onset multiple acyl CoA dehydrogenation deficiency associated with an abnormal isoenzyme pattern of serum lactate dehydrogenase

Authors:

Sugai F, Baba K, Toyooka K, Liang WC, Nishino I, Yamadera M, Sumi H, Fujimura H, Nishikawa Y.

Journal:

Neuromuscul Disord (2012): 159

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Fax
Email	sales@aatbio.com
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Page updated on October 8, 2026

Component A: Enzyme Mix	2 bottles (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: D-Lactate Standard	2.25 mg/vial