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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyzes the interconversion of pyruvate and lactate. LDH is present in cytosol of a wide variety of organisms, including animals and plants. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. LDH is also elevated in certain pathological conditions such as cancer. This Amplite® Lactate Dehydrogenase Assay Kit provides a fluorescence-based method for detecting D-lactate dehydrogenase (D-LDH) in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the concentration of NADH that is specifically monitored by a fluorogenic NADH sensor. This assay is specific for D-LDH. The fluorescence signal can be read by a fluorescence microplate reader. With this fluorimetric Amplite® D-lactate Dehydrogenase Assay Kit, we were able to detect as little as 1 mU/mL D-lactate dehydrogenase in a 100 µL reaction volume.
D-LDH dose response was measured with Amplite® Fluorimetric D-LDH Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader.
D-LDH dose response was measured with Amplite® Fluorimetric D-LDH Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader.
CatalogSize
Price
Quantity
13808200 Tests
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings

Fluorescence microplate reader
Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black
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Page updated on September 25, 2025