Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit is a fluorimetric assay kit for detecting D-LDH activity in serum, plasma, urine, and cell culture samples.

Detection Principle: Measures NADH generated by the D-LDH-coupled reaction.
Sensitivity: Detects D-LDH activity down to 1 mU/mL.
Specificity: Selective for D-lactate dehydrogenase.
Instrument Compatibility: Fluorescence detection at Ex/Em 540/590 nm.

D-LDH dose response was measured with Amplite® Fluorimetric D-LDH Assay Kit in a 96-well solid black plate using a Gemini (Molecular Devices) microplate reader.

Protocol

SDS

Catalog	Size	Price	Quantity
13808	200 Tests	Price

Overview

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyzes the interconversion of pyruvate and lactate. LDH is present in cytosol of a wide variety of organisms, including animals and plants. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. Quantification of LDH has a broad range of applications. LDH is also elevated in certain pathological conditions such as cancer. This Amplite® Lactate Dehydrogenase Assay Kit provides a fluorescence-based method for detecting D-lactate dehydrogenase (D-LDH) in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the concentration of NADH that is specifically monitored by a fluorogenic NADH sensor. This assay is specific for D-LDH. The fluorescence signal can be read by a fluorescence microplate reader. With this fluorimetric Amplite® D-lactate Dehydrogenase Assay Kit, we were able to detect as little as 1 mU/mL D-lactate dehydrogenase in a 100 µL reaction volume.

Citations

View all 7 citations: Citation Explorer

The Role of the ArcA Metabolic Regulator in Gram-Negative Bacteremia

Authors:

Brown, Aric

Arenobufagin inhibits prostate cancer epithelial-mesenchymal transition and metastasis by down-regulating β-catenin

Authors:

Chen, Liping and Mai, Weiqian and Chen, Minfeng and Hu, Jianyang and Zhuo, Zhenjian and Lei, Xueping and Deng, Lijuan and Liu, Junshan and Yao, Nan and Huang, Maohua and others, undefined

Journal:

Pharmacological Research (2017)

Fibroblast Activation Protein Alpha-Activated Tripeptide Bufadienolide Anti-tumor Prodrug with Reduced Cardiotoxicity

Authors:

Deng, Li-Juan and Wang, Long-Hai and Peng, Cheng-Kang and Li, Yi-Bin and Huang, Mao-Hua and Chen, Min-Feng and Lei, Xue-Ping and Qi, Ming and Cen, Yun and Ye, Wen-Cai and others, undefined

Journal:

Journal of Medicinal Chemistry (2017)

The use of KnockOut serum replacement (KSR) in three dimentional rat testicular cells co-culture model: An improved male reproductive toxicity testing system

Authors:

Zhang, Xiaofang and Wang, Lei and Zhang, Xiaodong and Ren, Lijun and Shi, Wenjing and Tian, Yijun and Zhu, Jiangbo and Zhang, Tianbao

Journal:

Food and Chemical Toxicology (2017)

Neuronal Culture Microenvironments Determine Preferences in Bioenergetic Pathway Use

Authors:

Sünwoldt, Juliane and Bosche, Bert and Meisel, Andreas and Mergenthaler, Philipp

Journal:

Frontiers in Molecular Neuroscience (2017): 305

References

View all 72 references: Citation Explorer

DNA binding, antioxidant, cytotoxicity (MTT, lactate dehydrogenase, NO), and cellular uptake studies of structurally different nickel(II) thiosemicarbazone complexes: synthesis, spectroscopy, electrochemistry, and X-ray crystallography

Authors:

Prabhakaran R, Kalaivani P, Huang R, Poornima P, Vijaya Padma V, Dallemer F, Natarajan K.

Journal:

J Biol Inorg Chem (2013): 233

Production of monoclonal antibodies for Plasmodium vivax lactate dehydrogenase and patient sera screening using sandwich ELISA

Authors:

Kim JH, Lee J, Sohn HJ, Song HO, Kim JY, Lee WJ, Park H, Shin HJ.

Journal:

Parasitol Res (2012): 1645

A lactate dehydrogenase ELISA-based assay for the in vitro determination of Plasmodium berghei sensitivity to anti-malarial drugs

Authors:

Orjuela-Sanchez P, Duggan E, Nolan J, Frangos JA, Carvalho LJ.

Journal:

Malar J (2012): 366

Association of degree and type of edema in posterior reversible encephalopathy syndrome with serum lactate dehydrogenase level: initial experience

Authors:

Gao B, Liu FL, Zhao B.

Journal:

Eur J Radiol (2012): 2844

Towards improved prognostic scores predicting survival in patients with brain metastases: a pilot study of serum lactate dehydrogenase levels

Authors:

Nieder C, Marienhagen K, Dalhaug A, Norum J.

Journal:

ScientificWorldJournal (2012): 609323

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	570 nm
Recommended plate	Solid black

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Page updated on October 8, 2026

Component A: Enzyme Probe	1 bottle (lyophilized powder)
Component B: Assay Buffer	1 bottle (10 mL)
Component C: NAD	1 vial
Component D: LDH	10 U/vial